BDNF ELISA Kit

Catalog No. ABIN411258

The Rat BDNF ELISA Kit (ABIN411258) is a Colorimetric ELISA Kit designed to quantify Rat BDNF. This ELISA Kit has been cited in 16 publications.

Quick Overview for BDNF ELISA Kit (ABIN411258)

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Binding Specificity: AA 129-247

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum

Product Details BDNF ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Purpose: Sandwich Rat BDNF ELISA Kit to quantitate Rat Bdnf in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA, citrate).

Analytical Method: Quantitative

Specificity: Expression system for standard: NS0
Immunogen sequence: H129-R247

Capture antibody and Detection antibody: monoclonal antibody from mouse, monoclonal antibody from mouse

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 15 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 5%, 5.5%, 7.8%
Inter-Assay Precision (CV%): 5%, 7%, 7.9%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for BDNF ELISA Kit (ABIN411258)

Duan, Lu, Wang, Zhang, Mao, Cao, Lin, Zhang, Shuai, Shen: "The long-term fate of mesenchymal stem cells labeled with magnetic resonance imaging-visible polymersomes in cerebral ischemia." in: International journal of nanomedicine, Vol. 12, pp. 6705-6719, (2018) (PubMed).

Sun, Zhu, Yin, Guo, Xu, Xiao, Jiang, Guo, Meng, Lu, Wang, Peng: "Differentiation of adipose-derived stem cells into Schwann cell-like cells through intermittent induction: potential advantage of cellular transient memory function." in: Stem cell research & therapy, Vol. 9, Issue 1, pp. 133, (2018) (PubMed).

Hibicke, Graham, Hayslett: "Adolescent chronic restraint stress (aCRS) elicits robust depressive-like behavior in freely cycling, adult female rats without increasing anxiety-like behaviors." in: Experimental and clinical psychopharmacology, Vol. 25, Issue 2, pp. 74-83, (2017) (PubMed).

Cai, Wu, Huang, Huang, Chen, Wang: "Effect of Zhuang Jing Decoction on Learning and Memory Ability in Aging Rats." in: Rejuvenation research, Vol. 19, Issue 4, pp. 303-8, (2017) (PubMed).

Liu, Zhang, Rong, Li, Wang: "Potassium 2-(1-hydroxypentyl)-benzoate attenuates neuronal apoptosis in neuron-astrocyte co-culture system through neurotrophy and neuroinflammation pathway." in: Acta pharmaceutica Sinica. B, Vol. 7, Issue 5, pp. 554-563, (2017) (PubMed).

Cheng, Li, Zhao, Yu, Yang, Wang, Chen, Yang: "Short-term sleep deprivation stimulates hippocampal neurogenesis in rats following global cerebral ischemia/reperfusion." in: PLoS ONE, Vol. 10, Issue 6, pp. e0125877, (2016) (PubMed).

Chung, Chung, Lee, Chung, Yang, Lee, Choi, Chang, Kim, Suh, Lee, Hwang, Do, Kim: "Expression of neurotrophic factors in injured spinal cord after transplantation of human-umbilical cord blood stem cells in rats." in: Journal of veterinary science, Vol. 17, Issue 1, pp. 97-102, (2016) (PubMed).

Azman, Zakaria, Abdul Aziz, Othman: "Tualang Honey Attenuates Noise Stress-Induced Memory Deficits in Aged Rats." in: Oxidative medicine and cellular longevity, Vol. 2016, pp. 1549158, (2016) (PubMed).

Dayi, Cetin, Sisman, Aksu, Tas, Gönenc, Uysal: "The effects of oxytocin on cognitive defect caused by chronic restraint stress applied to adolescent rats and on hippocampal VEGF and BDNF levels." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 21, pp. 69-75, (2015) (PubMed).

Sisman, Kiray, Camsari, Evren, Ates, Baykara, Aksu, Guvendi, Uysal: "Potential novel biomarkers for diabetic testicular damage in streptozotocin-induced diabetic rats: nerve growth factor Beta and vascular endothelial growth factor." in: Disease markers, Vol. 2014, pp. 108106, (2014) (PubMed).

Uysal, Kiray, Sisman, Camsari, Gencoglu, Baykara, Cetinkaya, Aksu: "Effects of voluntary and involuntary exercise on cognitive functions, and VEGF and BDNF levels in adolescent rats." in: Biotechnic & histochemistry : official publication of the Biological Stain Commission, Vol. 90, Issue 1, pp. 55-68, (2014) (PubMed).

Kranz, Warnecke, Lenarz, Durisin, Scheper: "Phosphodiesterase type 4 inhibitor rolipram improves survival of spiral ganglion neurons in vitro." in: PLoS ONE, Vol. 9, Issue 3, pp. e92157, (2014) (PubMed).

Uysal, Sisman, Dayi, Ozbal, Cetin, Baykara, Aksu, Tas, Cavus, Gonenc-Arda, Buyuk: "Acute footshock-stress increases spatial learning-memory and correlates to increased hippocampal BDNF and VEGF and cell numbers in adolescent male and female rats." in: Neuroscience letters, Vol. 514, Issue 2, pp. 141-6, (2012) (PubMed).

Aksu, Baykara, Ozbal, Cetin, Sisman, Dayi, Gencoglu, Tas, Büyük, Gonenc-Arda, Uysal: "Maternal treadmill exercise during pregnancy decreases anxiety and increases prefrontal cortex VEGF and BDNF levels of rat pups in early and late periods of life." in: Neuroscience letters, Vol. 516, Issue 2, pp. 221-5, (2012) (PubMed).

Uysal, Sisman, Dayi, Aksu, Cetin, Gencoglu, Tas, Buyuk: "Maternal exercise decreases maternal deprivation induced anxiety of pups and correlates to increased prefrontal cortex BDNF and VEGF." in: Neuroscience letters, Vol. 505, Issue 3, pp. 273-8, (2011) (PubMed).

Kobayashi, Yamagishi, Kamamoto, Yoshida, Uchino: "Effect of hyperthermia on hemopoietic stem cells increased thermal sensitivity along the stem cell differentiation pathway." in: Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society, Vol. 48, Issue 1, pp. 37-46, (1985) (PubMed).

Target Details for BDNF

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Alternative Name: BDNF

Background:

Background: Brain-derived neurotrophic factor (BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8 kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor (NGF). BDNF and neurotrophin-3 (NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. BDNF and other neurotrophins are critically involved in long-term potentiation (LTP). BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic (5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury.

Gene Full Name: brain-derived neurotrophic factor

Gene ID: 24225

UniProt: P23363

Pathways: RTK Signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity