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anti-Human CSF1R antibody for Intracellular Flow Cytometry

Recommended CSF1R Antibody (supplied by: Log in to see )

Antigen
Colony Stimulating Factor 1 Receptor (CSF1R) Antibodies
  • AI323359
  • c-fms
  • C-FMS
  • CD115
  • CSF-1-R
  • CSF-1R
  • CSF1
  • csf1r
  • Csfmr
  • CSFR
  • Fim-2
  • FIM2
  • fms
  • FMS
  • Fms
  • HDLS
  • M-CSF
  • M-CSF-R
  • M-CSFR
  • MGC89146
  • mrfms
  • panther
Reactivity
Human
283
187
59
21
12
9
7
4
4
4
2
2
2
2
1
Host
Rat
231
122
49
28
3
Clonality
Monoclonal
Conjugate
This CSF1R antibody is conjugated to PE
33
27
22
18
17
9
8
6
5
5
4
4
4
3
3
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Intracellular Flow Cytometry (ICFC)
280
205
163
123
42
39
26
20
18
15
12
11
9
7
4
3
2
2
2
2
1
Supplier
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Catalog No. ABIN2662951
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008737065 ABIN2662952 FACS ICFC PE Rat IgG1 kappa Log in to see 9-4D2-1E4 2

Top referenced anti-CSF1R antibody for Intracellular Flow Cytometry

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Application / Reactivity Human
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ELISA 158 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 81 Antibodies
Functional Studies (Func) 2 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 14 Antibodies
Immunofluorescence (IF) 37 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies
Immunohistochemistry (IHC) 84 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 41 Antibodies
Immunoprecipitation (IP) 16 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Neutralization (Neut) 2 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 210 Antibodies

General

Antigen Colony Stimulating Factor 1 Receptor (CSF1R) Antibodies
Reactivity Human
(283), (187), (59), (21), (12), (9), (7), (4), (4), (4), (2), (2), (2), (2), (1)
Host Rat
(231), (122), (49), (28), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CSF1R antibody is conjugated to PE
(33), (27), (22), (18), (17), (9), (8), (6), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Intracellular Flow Cytometry (ICFC)
(280), (205), (163), (123), (42), (39), (26), (20), (18), (15), (12), (11), (9), (7), (4), (3), (2), (2), (2), (2), (1)
Pubmed 2 references available
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Product Details anti-CSF1R Antibody

Target Details CSF1R Application Details Handling References for anti-CSF1R Antibody (ABIN2662951) Images
Purification The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Clone 9-4D2-1E4
Isotype IgG1 kappa

Target Details CSF1R

Product Details anti-CSF1R Antibody Application Details Handling References for anti-CSF1R Antibody (ABIN2662951) Images back to top
Antigen
Alternative Name CD115 (CSF1R Antibody Abstract)
Background CSF-1R, also known as CD115 and M-CSFR, is a single-pass type I membrane protein and member of the platelet-derived growth factor receptor family. Structural studies of CD115 have described an Ig-like extracellular domain, a transmembrane domain, an intracellular juxtamembrane domain, a split tyrosine kinase domain, and a C-terminal tail receptor. Receptor activation induces homodimerization in addition to phosphorylation and ubiquitinylation of intracellular residues. The natural ligands of CD115 include M-CSF and IL-34. CD115 directly influences tissue macrophage and osteoclast differentiation and proliferation. It is expressed on monocytes/macrophages, plasmacytoid and conventional dendritic cells, and osteoclasts.
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways RTK Signaling

Application Details

Product Details anti-CSF1R Antibody Target Details CSF1R Handling References for anti-CSF1R Antibody (ABIN2662951) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CSF1R Antibody Target Details CSF1R Application Details References for anti-CSF1R Antibody (ABIN2662951) Images back to top
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-CSF1R Antibody (ABIN2662951)

Product Details anti-CSF1R Antibody Target Details CSF1R Application Details Handling Images back to top
Background publications

Roussel, Sherr: "Mouse NIH 3T3 cells expressing human colony-stimulating factor 1 (CSF-1) receptors overgrow in serum-free medium containing human CSF-1 as their only growth factor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 20, pp. 7924-7, 1989

Sherr, Ashmun, Downing, Ohtsuka, Quan, Golde, Roussel: "Inhibition of colony-stimulating factor-1 activity by monoclonal antibodies to the human CSF-1 receptor." in: Blood, Vol. 73, Issue 7, pp. 1786-93, 1989

Images

Product Details anti-CSF1R Antibody Target Details CSF1R Application Details Handling References for anti-CSF1R Antibody (ABIN2662951) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CSF1R antibody (Colony Stimulating Factor 1 Receptor)  (PE) (ABIN2662951) anti-Colony Stimulating Factor 1 Receptor (CSF1R) antibody (PE)