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|Application / Reactivity||Human|
|Enzyme Immunoassay (EIA)||7 Antibodies|
|Flow Cytometry (FACS)||18 Antibodies|
|Immunocytochemistry (ICC)||9 Antibodies|
|Immunofluorescence (IF)||22 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||2 Antibodies|
|Immunohistochemistry (IHC)||51 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||24 Antibodies|
|Immunoprecipitation (IP)||1 Antibodies|
|Western Blotting (WB)||107 Antibodies|
|Antigen||EPH Receptor B2 (EPHB2) Antibodies|
|Conjugate||This EPH Receptor B2 antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-EPH Receptor B2 AntibodyTarget Details EPH Receptor B2 Application Details Handling References for anti-EPH Receptor B2 Antibody (ABIN391921) Images
|Specificity||This EphB2 antibody is generated from rabbits immunized with a recombinant human EphB2 protein.|
|Purification||This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Immunogen||This EphB2 antibody is generated from rabbits immunized with a recombinant human EphB2 protein.|
Target Details EPH Receptor B2Product Details anti-EPH Receptor B2 Antibody Application Details Handling References for anti-EPH Receptor B2 Antibody (ABIN391921) Images back to top
|Alternative Name||EphB2 (EPHB2 Antibody Abstract)|
Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The ligand-activated form of EphB2, which belongs to the Tyr family of protein kinases, interacts with multiple proteins, including GTPase-activating protein (RASGAP) through its SH2 domain. It binds RASGAP through the juxtamembrane tyrosines residues, and also interacts with PRKCABP and GRIP1 This type I membrane protein is expressed in brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. It is preferentially expressed in fetal brain. This protein contains putatively 2 fibronectin type III domains and 1 sterile alpha motif (SAM) domain.
Synonyms: Ephrin type-B receptor 2, Tyrosine-protein kinase receptor EPH-3, DRT, Receptor protein-tyrosine kinase HEK5, ERK
|Molecular Weight||117506 DA|
|Research Area||Phospho-specific antibodies, Stem Cells, Cell Signaling, Protein Modifications, Cell Structure, Neurology|
Application DetailsProduct Details anti-EPH Receptor B2 Antibody Target Details EPH Receptor B2 Handling References for anti-EPH Receptor B2 Antibody (ABIN391921) Images back to top
|Application Notes||WB = 1:1000, IHC (p) = 1:10-50, FACS = 1:10-50|
|Restrictions||For Research Use only|
HandlingProduct Details anti-EPH Receptor B2 Antibody Target Details EPH Receptor B2 Application Details References for anti-EPH Receptor B2 Antibody (ABIN391921) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
|Storage Comment||Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.|
|Expiry Date||6 months|
References for anti-EPH Receptor B2 Antibody (ABIN391921)Product Details anti-EPH Receptor B2 Antibody Target Details EPH Receptor B2 Application Details Handling Images back to top
Thanos, Goodwill, Bowie: "Oligomeric structure of the human EphB2 receptor SAM domain." in: Science (New York, N.Y.), Vol. 283, Issue 5403, pp. 833-6, 1999 (PubMed).
Ikegaki, Tang, Liu et al.: "Molecular characterization and chromosomal localization of DRT (EPHT3): a developmentally regulated human protein-tyrosine kinase gene of the EPH family." in: Human molecular genetics, Vol. 4, Issue 11, pp. 2033-45, 1996 (PubMed).
Iwase, Tanaka, Suzuki et al.: "Identification of protein-tyrosine kinase genes preferentially expressed in embryo stomach and gastric cancer." in: Biochemical and biophysical research communications, Vol. 194, Issue 2, pp. 698-705, 1993 (PubMed).
ImagesProduct Details anti-EPH Receptor B2 Antibody Target Details EPH Receptor B2 Application Details Handling References for anti-EPH Receptor B2 Antibody (ABIN391921) back to top