anti-Human EPH Receptor B2 antibody for Flow Cytometry

Recommended EPH Receptor B2 Antibody (supplied by: Log in to see )

Antigen
EPH Receptor B2 (EPHB2) Antibodies
  • CAPB
  • DRT
  • EK5
  • EPHT3
  • ERK
  • Hek5
  • PCBC
  • Tyro5
  • Cek5
  • Drt
  • ETECK
  • Erk
  • Nuk
  • Prkm5
  • Qek5
  • Sek3
  • RGD1564232
  • EPH receptor B2
  • Eph receptor B2
  • EPHB2
  • Ephb2
Reactivity
Human
164
59
5
3
3
3
3
3
2
2
1
1
1
1
1
Host
Rabbit
82
44
25
20
Clonality
Polyclonal
Conjugate
This EPH Receptor B2 antibody is un-conjugated
10
7
6
6
6
6
3
2
2
2
2
2
2
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
120
92
51
43
30
29
15
7
2
2
1
1
1
1
1
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Catalog No. ABIN391921
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.588789 ABIN4897095 FACS APC Rat IgG2a AA 27-548 Log in to see 512012 1
13.588789 ABIN4897097 FACS Alexa Fluor 488 Rat IgG2a AA 27-548 Log in to see 512012 1
13.588789 ABIN4900039 CyTOF FACS ICC Rat IgG2a AA 27-548 Log in to see 512012 1
13.588789 ABIN4900040 CyTOF FACS ICC IHC SimWes WB Goat IgG AA 27-548 Log in to see Polyclonal 19
13.588789 ABIN4897096 FACS PerCP Rat IgG2a AA 27-548 Log in to see 512012
13.588789 ABIN4897098 FACS PE Rat IgG2a AA 27-548 Log in to see 512012
13.588789 ABIN1997418 FACS PE Rabbit IgG AA 1-543 Log in to see 001
13.588789 ABIN3030824 FACS IHC ELISA WB Mouse IgG1, kappa AA 127-425 Log in to see 48CT12-6-4
1 ABIN387812 FACS IHC (p) WB Mouse IgG1 kappa Log in to see 48CT12-6-4 5
1 ABIN958114 ICC FACS IHC WB Goat IgG AA 27-548 Log in to see Polyclonal
1 ABIN1830335 FACS PerCP Rat IgG2a AA 27-551 Log in to see 12K86
1 ABIN1830336 FACS PE Rat IgG2a AA 27-551 Log in to see 12K86
1 ABIN4308700 FACS Alexa Fluor 750 Rat IgG2a AA 27-548 Log in to see 512012
1 ABIN4308699 FACS Alexa Fluor 647 Rat IgG2a AA 27-548 Log in to see 512012
1 ABIN4308698 FACS Alexa Fluor 700 Rat IgG2a AA 27-548 Log in to see 512012
1 ABIN4308701 FACS Alexa Fluor 594 Rat IgG2a AA 27-548 Log in to see 512012
1 ABIN4308702 FACS Alexa Fluor 350 Rat IgG2a AA 27-548 Log in to see 512012
1 ABIN4308703 FACS Alexa Fluor 405 Rat IgG2a AA 27-548 Log in to see 512012
1 ABIN2689499 IHC (fro) FACS IHC (p) Mouse IgG1, kappa Log in to see 2H9 4
1 ABIN5536996 FACS IHC (p) WB Rabbit Ig Fraction Log in to see Polyclonal

General

Antigen EPH Receptor B2 (EPHB2) Antibodies
Reactivity Human
(164), (59), (5), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(82), (44), (25), (20)
Clonality (Clone)
Polyclonal   ( )
Conjugate This EPH Receptor B2 antibody is un-conjugated
(10), (7), (6), (6), (6), (6), (3), (2), (2), (2), (2), (2), (2), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(120), (92), (51), (43), (30), (29), (15), (7), (2), (2), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-EPH Receptor B2 Antibody

Target Details EPH Receptor B2 Application Details Handling References for anti-EPH Receptor B2 antibody (ABIN391921) Images
Specificity This EphB2 antibody is generated from rabbits immunized with a recombinant human EphB2 protein.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This EphB2 antibody is generated from rabbits immunized with a recombinant human EphB2 protein.
Clone RB15188
Isotype Ig Fraction

Target Details EPH Receptor B2

Product Details anti-EPH Receptor B2 Antibody Application Details Handling References for anti-EPH Receptor B2 antibody (ABIN391921) Images back to top
Antigen
Alternative Name EphB2 (EPHB2 Antibody Abstract)
Background Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The ligand-activated form of EphB2, which belongs to the Tyr family of protein kinases, interacts with multiple proteins, including GTPase-activating protein (RASGAP) through its SH2 domain. It binds RASGAP through the juxtamembrane tyrosines residues, and also interacts with PRKCABP and GRIP1 This type I membrane protein is expressed in brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. It is preferentially expressed in fetal brain. This protein contains putatively 2 fibronectin type III domains and 1 sterile alpha motif (SAM) domain.
Synonyms: Ephrin type-B receptor 2, Tyrosine-protein kinase receptor EPH-3, DRT, Receptor protein-tyrosine kinase HEK5, ERK
Molecular Weight 117506 DA
Gene ID 2048
UniProt P29323
Research Area Phospho-specific antibodies, Stem Cells, Cell Signaling, Protein Modifications, Cell Structure, Neurology
Pathways RTK Signaling, Regulation of long-term Neuronal Synaptic Plasticity

Application Details

Product Details anti-EPH Receptor B2 Antibody Target Details EPH Receptor B2 Handling References for anti-EPH Receptor B2 antibody (ABIN391921) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:10-50, FACS = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-EPH Receptor B2 Antibody Target Details EPH Receptor B2 Application Details References for anti-EPH Receptor B2 antibody (ABIN391921) Images back to top
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-EPH Receptor B2 antibody (ABIN391921)

Product Details anti-EPH Receptor B2 Antibody Target Details EPH Receptor B2 Application Details Handling Images back to top
Background publications

Thanos, Goodwill, Bowie: "Oligomeric structure of the human EphB2 receptor SAM domain." in: Science (New York, N.Y.), Vol. 283, Issue 5403, pp. 833-6, 1999

Ikegaki, Tang, Liu, Biegel, Allen, Yoshioka, Sulman, Brodeur, Pleasure: "Molecular characterization and chromosomal localization of DRT (EPHT3): a developmentally regulated human protein-tyrosine kinase gene of the EPH family." in: Human molecular genetics, Vol. 4, Issue 11, pp. 2033-45, 1996

Iwase, Tanaka, Suzuki, Naito, Sugimura, Kino: "Identification of protein-tyrosine kinase genes preferentially expressed in embryo stomach and gastric cancer." in: Biochemical and biophysical research communications, Vol. 194, Issue 2, pp. 698-705, 1993

Images

Product Details anti-EPH Receptor B2 Antibody Target Details EPH Receptor B2 Application Details Handling References for anti-EPH Receptor B2 antibody (ABIN391921) back to top
Supplier Images
Western Blotting (WB) image for anti-EPH Receptor B2 (EPHB2) antibody (ABIN391921) Western blot analysis of EphB2(arrow) using rabbit polyclonal EphB2 Antibody(ABIN3919...
Immunohistochemistry (IHC) image for anti-EPH Receptor B2 (EPHB2) antibody (ABIN391921) Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with EphB2 a...
Flow Cytometry (FACS) image for anti-EPH Receptor B2 (EPHB2) antibody (ABIN391921) Flow cytometric analysis of NCI-H460 cells using EphB2 Antibody (bottom histogram) co...