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Rat (Rattus) Ephrin A5 ELISA Kit

Recommended Ephrin A5 Product (supplied by: Log in to see )

Antigen
Ephrin A5 (EFNA5) ELISA Kits
  • AF1
  • af1
  • AL-1
  • AV158822
  • EFL-5
  • EFL5
  • efl5
  • EFNA5
  • Ephrin-A5
  • Epl7
  • eplg7
  • EPLG7
  • GLC1M
  • LERK-7
  • LERK7
  • Lerk7
  • lerk7
  • RAGS
  • rags
Reactivity
Human, Mouse (Murine), Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
7
1
1
1
1
Methode Type
Cell ELISA
Application
ELISA
Supplier
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Catalog No. ABIN2113794
$ 528.57
Plus shipping costs $45.00

Similar Ephrin A5 ELISA Kits

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
ELISA 6 ELISA Kits 1 ELISA Kits 1 ELISA Kits

General

Antigen Ephrin A5 (EFNA5) ELISA Kits
Reactivity Human, Mouse (Murine), Rat (Rattus)
Kits with alternative reactivity to:
(7), (1), (1), (1), (1)
Methode Type Cell ELISA
Application ELISA
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Product Details Ephrin A5 ELISA Kit

Target details Application Details Handling Images
Detection Method Colorimetric
Characteristics Assay Type: Cell-Based

Target details

Product Details Ephrin A5 ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name EFNA5 (EFNA5 ELISA Kit Abstract)
Background Synonyms: EPH-related receptor tyrosine kinase ligand 7, LERK-7, AL-1
Gene Symbol: EFNA5
Gene ID 1946
UniProt P52803
Pathways RTK Signaling

Application Details

Product Details Ephrin A5 ELISA Kit Target details Handling Images back to top
Assay Procedure
  • Remove the coating solution and wash the plate three times by filling the wells with 100 μL PBS-0.05 % Tween20. The solutions or washes are removed by flicking the plate over a sink. The remaining drops are removed by patting the plate on a paper towel.
  • Block the remaining protein-binding sites in the coated wells by adding 100 μL blocking buffer, 3 % skim milk in PBS per well. Incubate for 1 hour at RT with gentle shaking.
  • Wash the plate three times with 100 μL PBS-0.05 % Tween 20.
  • Add 50 μL of diluted antibody to each well. Incubate the plate at 37 °C for an hour with gentle shaking.
  • Wash the plate six times with 100 μL PBS-0.05 %Tween 20.
  • Add 50 μL of conjugated secondary antibody, diluted at the optimal concentration (according to the manufacturer) in blocking buffer immediately before use. Incubate at 37 °C for an hour.
  • Wash the plate six times with 100 μL PBS-0.05 %Tween20.
  • Prepare the substrate solution by mixing acetic acid, TMB and 0.03 % H2O2 with the volume ratio of 4:1:5.
  • Dispense 50 μL of the substrate solution per well with a multichannel pipe. Incubate the plate at 37 °C in dark for 15-30 mins.
  • After sufficient color development, add 100 μL of stop solution to the wells (if necessary).
  • Read the absorbance (optical density at 450nm) of each well with a plate reader.
Restrictions For Research Use only

Handling

Product Details Ephrin A5 ELISA Kit Target details Application Details Images back to top
Handling Advice Avoid multiple freeze-thaw cycles
Storage 4 °C
Storage Comment Store at 4 °C for frequent use, at -20°C for infrequent use.
Expiry Date 6 months