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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)||Human||Cow (Bovine)||Chicken||Xenopus laevis||Zebrafish (Danio rerio)|
|Western Blotting (WB)||18 Antibodies||27 Antibodies||57 Antibodies||3 Antibodies||6 Antibodies||3 Antibodies||5 Antibodies|
|Immunoprecipitation (IP)||2 Antibodies||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||2 Antibodies||4 Antibodies||1 Antibodies|
|Immunohistochemistry (IHC)||8 Antibodies||3 Antibodies||2 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunofluorescence (IF)||1 Antibodies||1 Antibodies|
|Antigen||Ephrin B1 (EFNB1) Antibodies|
|Reactivity||Cow (Bovine), Chicken, Human, Mouse (Murine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio) Alternatives|
|Conjugate||This Ephrin B1 antibody is un-conjugated Alternatives|
Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-Ephrin B1 AntibodyTarget Details Ephrin B1 Application Details Handling References for anti-Ephrin B1 Antibody (ABIN372620) Images
|Specificity||Specific for the ~46k Ephrin-B protein phosphorylated at Tyr317. Immunolabeling of the Ephrin-B band is blocked by lambda-phosphatase treatment|
Species reactivity (expected):Human, Bovine, Chicken, Mouse, Xenopus and Zebrafish.
Species reactivity (tested):Rat.
|Purification||Sequential Chromatography on phospho- and dephosphopeptide affinity columns.|
|Immunogen||Phosphopeptide corresponding to amino acid residues surrounding the phospho Tyr317 of Chicken Ephrin-B.|
Target Details Ephrin B1Product Details anti-Ephrin B1 Antibody Application Details Handling References for anti-Ephrin B1 Antibody (ABIN372620) Images back to top
|Alternative Name||Ephrin-B1 (EFNB1 Antibody Abstract)|
|Background||Ephrin-B proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development (Flanagan and Vancerhaeghen, 1998, Dufour et al., 2003, Oike et al., 2002). Ephrin-B molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cell-to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing Ephrin-B on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of Ephrin-B is thought to be a critical component of this reverse signaling event (Palmer et al., 2002). Recent work suggests that phosphorylation of a specific Ephrin-B residue (Tyr298) plays a key role in Ephrin-B signaling (Kalo, et al., 2001).Synonyms: CEK5 LIgand, CEK5-L, EFL-3, EFL3, EFNB1, ELK-L, EPH-related receptor tyrosine kinase ligand 2, EPLG2, LERK-2, LERK2|
Application DetailsProduct Details anti-Ephrin B1 Antibody Target Details Ephrin B1 Handling References for anti-Ephrin B1 Antibody (ABIN372620) Images back to top
Western Blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Ephrin B1 Antibody Target Details Ephrin B1 Application Details References for anti-Ephrin B1 Antibody (ABIN372620) Images back to top
|Buffer||10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Store the antibody undiluted (in aliquots) at-20 °C.|
References for anti-Ephrin B1 Antibody (ABIN372620)Product Details anti-Ephrin B1 Antibody Target Details Ephrin B1 Application Details Handling Images back to top
Bong, Park, Lee, Mood, Ishimura, Daar: "Tyr-298 in ephrinB1 is critical for an interaction with the Grb4 adaptor protein." in: The Biochemical journal, Vol. 377, Issue Pt 2, pp. 499-507, 2004
Dufour, Seibt, Passante, Depaepe, Ciossek, Frisén, Kullander, Flanagan, Polleux, Vanderhaeghen: "Area specificity and topography of thalamocortical projections are controlled by ephrin/Eph genes." in: Neuron, Vol. 39, Issue 3, pp. 453-65, 2003
Palmer, Zimmer, Erdmann, Eulenburg, Porthin, Heumann, Deutsch, Klein: "EphrinB phosphorylation and reverse signaling: regulation by Src kinases and PTP-BL phosphatase." in: Molecular cell, Vol. 9, Issue 4, pp. 725-37, 2002
Oike, Ito, Hamada, Zhang, Miyata, Arai, Inada, Araki, Nakagata, Takeya, Kisanuki, Yanagisawa, Gale, Suda: "Regulation of vasculogenesis and angiogenesis by EphB/ephrin-B2 signaling between endothelial cells and surrounding mesenchymal cells." in: Blood, Vol. 100, Issue 4, pp. 1326-33, 2002
Flanagan, Vanderhaeghen: "The ephrins and Eph receptors in neural development." in: Annual review of neuroscience, Vol. 21, pp. 309-45, 1998
ImagesProduct Details anti-Ephrin B1 Antibody Target Details Ephrin B1 Application Details Handling References for anti-Ephrin B1 Antibody (ABIN372620) back to top