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anti-Xenopus laevis Ephrin B1 antibody for Western Blotting

Recommended Ephrin B1 Antibody (supplied by: Log in to see )

Antigen
Ephrin B1 (EFNB1) Antibodies
  • Cek5-L
  • cfnd
  • CFND
  • cfns
  • CFNS
  • EFL-3
  • efl3
  • EFL3
  • EFNB1
  • efnb1-A
  • Elk-L
  • ephrinB1
  • Epl2
  • EPLG2
  • eplg2
  • Eplg2
  • LERK-2
  • lerk2
  • LERK2
  • Lerk2
  • Stra1
Epitope
pTyr331
17
11
11
9
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Chicken, Human, Mouse (Murine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
69
32
22
6
4
2
2
2
1
1
1
Host
Rabbit
74
2
2
1
Clonality
Polyclonal
Conjugate
This Ephrin B1 antibody is un-conjugated
2
2
2
2
2
2
Application
Western Blotting (WB)
65
40
10
5
4
2
2
2
1
Supplier
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Catalog No. ABIN372621
$ 412.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0011806369 ABIN372620 WB Rabbit IgG pTyr317 Log in to see Polyclonal 5
0.0011806369 ABIN372619 WB Rabbit IgG pTyr298 Log in to see Polyclonal 5

Top referenced anti-Ephrin B1 antibody for Western Blotting

Similar anti-Ephrin B1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine) Human Cow (Bovine) Chicken Xenopus laevis Zebrafish (Danio rerio)
Western Blotting (WB) 19 Antibodies 28 Antibodies 59 Antibodies 3 Antibodies 6 Antibodies 3 Antibodies 5 Antibodies
Immunoprecipitation (IP) 3 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies 4 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 9 Antibodies 4 Antibodies 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies 1 Antibodies
Immunofluorescence (IF) 1 Antibodies 1 Antibodies

General

Antigen Ephrin B1 (EFNB1) Antibodies
Epitope pTyr331
(17), (11), (11), (9), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Chicken, Human, Mouse (Murine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
(69), (32), (22), (6), (4), (2), (2), (2), (1), (1), (1)
Host Rabbit
(74), (2), (2), (1)
Clonality
Polyclonal
Conjugate This Ephrin B1 antibody is un-conjugated
(2), (2), (2), (2), (2), (2)
Application Western Blotting (WB)
(65), (40), (10), (5), (4), (2), (2), (2), (1)
Pubmed 5 references available
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Product Details anti-Ephrin B1 Antibody

Target Details Ephrin B1 Application Details Handling References for anti-Ephrin B1 Antibody (ABIN372621) Images
Specificity Specific for the ~46k Ephrin-B protein phosphorylated at Tyr331. Immunolabeling of the Ephrin-B band is blocked by lambda-phosphatase treatment.
Cross-Reactivity (Details) Species reactivity (expected):Human, Bovine, Chicken, Mouse, Xenopus and Zebrafish.
Species reactivity (tested):Rat.
Purification Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
Immunogen Phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr331 of Chicken Ephrin-B.
Isotype IgG

Target Details Ephrin B1

Product Details anti-Ephrin B1 Antibody Application Details Handling References for anti-Ephrin B1 Antibody (ABIN372621) Images back to top
Antigen
Alternative Name Ephrin-B1 (EFNB1 Antibody Abstract)
Background Ephrin-B proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development (Flanagan and Vancerhaeghen, 1998, Dufour et al., 2003, Oike et al., 2002). Ephrin-B molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cell-to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing Ephrin-B on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of Ephrin-B is thought to be a critical component of this reverse signaling event (Palmer et al., 2002). Recent work suggests that phosphorylation of a specific Ephrin-B residue (Tyr298) plays a key role in Ephrin-B signaling (Kalo, et al., 2001).Synonyms: CEK5 LIgand, CEK5-L, EFL-3, EFL3, EFNB1, ELK-L, EPH-related receptor tyrosine kinase ligand 2, EPLG2, LERK-2, LERK2
Gene ID 395896
NCBI Accession NP_990366
UniProt O73612
Pathways RTK Signaling

Application Details

Product Details anti-Ephrin B1 Antibody Target Details Ephrin B1 Handling References for anti-Ephrin B1 Antibody (ABIN372621) Images back to top
Application Notes Western Blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Ephrin B1 Antibody Target Details Ephrin B1 Application Details References for anti-Ephrin B1 Antibody (ABIN372621) Images back to top
Format Liquid
Buffer 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at-20 °C.

References for anti-Ephrin B1 Antibody (ABIN372621)

Product Details anti-Ephrin B1 Antibody Target Details Ephrin B1 Application Details Handling Images back to top
Background publications

Bong, Park, Lee, Mood, Ishimura, Daar: "Tyr-298 in ephrinB1 is critical for an interaction with the Grb4 adaptor protein." in: The Biochemical journal, Vol. 377, Issue Pt 2, pp. 499-507, 2004

Dufour, Seibt, Passante, Depaepe, Ciossek, Frisén, Kullander, Flanagan, Polleux, Vanderhaeghen: "Area specificity and topography of thalamocortical projections are controlled by ephrin/Eph genes." in: Neuron, Vol. 39, Issue 3, pp. 453-65, 2003

Palmer, Zimmer, Erdmann, Eulenburg, Porthin, Heumann, Deutsch, Klein: "EphrinB phosphorylation and reverse signaling: regulation by Src kinases and PTP-BL phosphatase." in: Molecular cell, Vol. 9, Issue 4, pp. 725-37, 2002

Oike, Ito, Hamada, Zhang, Miyata, Arai, Inada, Araki, Nakagata, Takeya, Kisanuki, Yanagisawa, Gale, Suda: "Regulation of vasculogenesis and angiogenesis by EphB/ephrin-B2 signaling between endothelial cells and surrounding mesenchymal cells." in: Blood, Vol. 100, Issue 4, pp. 1326-33, 2002

Flanagan, Vanderhaeghen: "The ephrins and Eph receptors in neural development." in: Annual review of neuroscience, Vol. 21, pp. 309-45, 1998

Images

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Supplier Images
 image for anti-Ephrin B1 antibody (EFNB1) (pTyr331) (ABIN372621) anti-Ephrin B1 (EFNB1) (pTyr331) antibody