anti-Xenopus laevis Ephrin B1 antibody for Western Blotting

Recommended Ephrin B1 Antibody (supplied by: Log in to see )

Antigen
Ephrin B1 (EFNB1) Antibodies
  • EFNB1
  • LERK2
  • Cek5-L
  • EFL-3
  • Elk-L
  • Epl2
  • Eplg2
  • LERK-2
  • Lerk2
  • Stra1
  • CFND
  • CFNS
  • EFL3
  • EPLG2
  • cfnd
  • cfns
  • efl3
  • efnb1-A
  • ephrinB1
  • eplg2
  • lerk2
  • ephrin-B1
  • ephrin B1
  • EFNB1
  • Efnb1
  • efnb1
Epitope
pTyr298
18
11
10
8
5
5
3
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Non-Human Primate, Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
63
40
33
7
4
3
3
2
2
2
2
2
Host
Rabbit
68
2
1
1
Clonality
Polyclonal
Conjugate
This Ephrin B1 antibody is un-conjugated
2
2
2
2
2
2
Application
Western Blotting (WB)
63
36
6
6
3
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Catalog No. ABIN5551543
$ 375.38
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5551544 WB Rabbit IgG pTyr317 Log in to see Polyclonal
1 ABIN5551545 WB Rabbit IgG pTyr331 Log in to see Polyclonal

General

Antigen Ephrin B1 (EFNB1) Antibodies
Epitope pTyr298
(18), (11), (10), (8), (5), (5), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Non-Human Primate, Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
(63), (40), (33), (7), (4), (3), (3), (2), (2), (2), (2), (2)
Host Rabbit
(68), (2), (1), (1)
Clonality
Polyclonal
Conjugate This Ephrin B1 antibody is un-conjugated
(2), (2), (2), (2), (2), (2)
Application Western Blotting (WB)
(63), (36), (6), (6), (3), (1), (1), (1), (1)
Supplier Log in to see

Product Details anti-Ephrin B1 Antibody

Target Details Ephrin B1 Application Details Handling Images
Specificity Specific for the ~46k Ephrin-B protein phosphorylated at Tyr298. Immunolabeling of the Ephrin-B band is blocked by lambda-phosphatase treatment.
Cross-Reactivity (Details) Species reactivity (expected):Human, Bovine, Canine, Chicken, Mouse, Xenopus, Zebrafish and non-Human Primates.
Species reactivity (tested):Rat.
Purification Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
Immunogen Phosphopeptide corresponding to amino acid residues surrounding the phospho Tyr298 of Xenopus Ephrin-B.
Isotype IgG

Target Details Ephrin B1

Product Details anti-Ephrin B1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Ephrin-B1 (EFNB1 Antibody Abstract)
Background Ephrin-B proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development (Flanagan and Vancerhaeghen, 1998, Dufour et al., 2003, Oike et al., 2002). Ephrin-B molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cell-to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing Ephrin-B on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of Ephrin-B is thought to be a critical component of this reverse signaling event (Palmer et al., 2002). Recent work suggests that phosphorylation of a specific Ephrin-B residue (Tyr298) plays a key role in Ephrin-B signaling (Kalo, et al., 2001).Synonyms: CEK5 LIgand, CEK5-L, EFL-3, EFL3, EFNB1, ELK-L, EPH-related receptor tyrosine kinase ligand 2, EPLG2, LERK-2, LERK2
Gene ID 394292
NCBI Accession NP_001080948
UniProt O13097
Pathways RTK Signaling

Application Details

Product Details anti-Ephrin B1 Antibody Target Details Ephrin B1 Handling Images back to top
Application Notes Western Blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Ephrin B1 Antibody Target Details Ephrin B1 Application Details Images back to top
Format Liquid
Buffer 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at-20 °C.