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Rat (Rattus) FGF1 ELISA Kit

Recommended FGF1 Product (supplied by: Log in to see )

Antigen
Fibroblast Growth Factor 1 (Acidic) (FGF1) ELISA Kits
  • AFGF
  • aFGF
  • Dffrx
  • ECGF
  • ecgf
  • ecgf-beta
  • ECGF-beta
  • ECGFA
  • ecgfa
  • ecgfb
  • ECGFB
  • Fam
  • Fgf-1
  • FGF-1
  • fgf-1
  • fgf-alpha
  • FGF-alpha
  • FGF1
  • Fgf1
  • fgfa
  • Fgfa
  • FGFA
  • GLIO703
  • glio703
  • HBGF-1
  • HBGF1
  • hbgf1
  • LOC100221393
  • zgc:136885
Epitope
AA 16-155
Reactivity
Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
15
14
14
9
9
7
6
4
3
3
3
3
1
1
Methode Type
Sandwich ELISA
Detection Range
31.2-2000 pg/mL
Minimum Detection Limit
31.2 pg/mL
Application
ELISA
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Catalog No. ABIN1889350
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32693866 ABIN416310 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see 1
0.32693866 ABIN2708640 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012117105 ABIN365584 Sandwich ELISA Serum, Plasma, Tissue Homogenate 23.44-1500 pg/mL 23.44 pg/mL Log in to see
0.0012117105 ABIN771375 Competition ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids Log in to see
0.0012117105 ABIN1569784 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
0.0012117105 ABIN1884747 Sandwich ELISA 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.0012117105 ABIN2684254 Sandwich ELISA Plasma (heparin), Plasma (EDTA), Cell Culture Supernatant, Serum 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012117105 ABIN579301 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012117105 ABIN2540985 Serum, Plasma, Biological Fluids 2.8-150 pg/mL 2.8 pg/mL Log in to see
0.0012117105 ABIN2533367 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.62-1000 pg/mL 15.62 pg/mL Log in to see
0.0012117105 ABIN2050208 Sandwich ELISA Log in to see
0.0012117105 ABIN2050226 Sandwich ELISA Log in to see

Top referenced FGF1 ELISA Kit for ELISA

Similar FGF1 ELISA Kits

Application / Reactivity Rat (Rattus)
ELISA 13 ELISA Kits

General

Antigen Fibroblast Growth Factor 1 (Acidic) (FGF1) ELISA Kits
Epitope AA 16-155
Reactivity Rat (Rattus)
Kits with alternative reactivity to:
(15), (14), (14), (9), (9), (7), (6), (4), (3), (3), (3), (3), (1), (1)
Methode Type Sandwich ELISA
Detection Range 31.2-2000 pg/mL
Minimum Detection Limit 31.2 pg/mL
Application ELISA
Pubmed 2 references available
Supplier Log in to see

Product Details FGF1 ELISA Kit

Target details Application Details Handling References for FGF1 Kit (ABIN1889350) Images
Purpose For quantitative detection of rat FGF1 in cell culture supernates, serum and plasma(heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant rat FGF1
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- rat FGF1 antibod - 1
  • Lyophilized recombinant rat FGF1 standar - 10ng/tubex2
  • Biotinylated anti- rat FGF1 antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: F16-D155

Target details

Product Details FGF1 ELISA Kit Application Details Handling References for FGF1 Kit (ABIN1889350) Images back to top
Antigen
Alternative Name FGF1 (FGF1 ELISA Kit Abstract)
Background Heparin-binding growth factor 1 is a protein that in humans is encoded by the FGF1 gene. The protein encoded by this gene is a member of the fibroblast growth factor(FGF) family. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. The FGF1 gene was mapped to chromosome 5q31.3-q33.2 by in situ hybridization.
Gene ID 25317
UniProt P61149
Research Area Cancer, Angiogenesis, Growth Factors
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details FGF1 ELISA Kit Target details Handling References for FGF1 Kit (ABIN1889350) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol rat FGF1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FGF1 has been precoated onto 96-well plates. Standards(E.coli, F16-D155) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FGF1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat FGF1 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL rat FGF1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each rat FGF1 standard solution and each sample is measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 156, Standard deviation: 7.49, CV(%): 4.8
  • Sample 2: n=16, Mean(pg/ml): 758, Standard deviation: 56.85, CV(%): 7.5
  • Sample 3: n=16, Mean(pg/ml): 1229, Standard deviation: 82.34, CV(%): 6.7,
  • Sample 1: n=24, Mean(pg/ml): 157, Standard deviation: 13.82, CV(%): 8.8
  • Sample 2: n=24, Mean(pg/ml): 749, Standard deviation: 65.16, CV(%): 8.7
  • Sample 3: n=24, Mean(pg/ml): 1226, Standard deviation: 91.95, CV(%): 7.5
Restrictions For Research Use only

Handling

Product Details FGF1 ELISA Kit Target details Application Details References for FGF1 Kit (ABIN1889350) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for FGF1 Kit (ABIN1889350)

Product Details FGF1 ELISA Kit Target details Application Details Handling Images back to top
Background publications

Dionne, Crumley, Bellot, Kaplow, Searfoss, Ruta, Burgess, Jaye, Schlessinger: "Cloning and expression of two distinct high-affinity receptors cross-reacting with acidic and basic fibroblast growth factors." in: The EMBO journal, Vol. 9, Issue 9, pp. 2685-92, 1990

Jaye, Howk, Burgess, Ricca, Chiu, Ravera, OBrien, Modi, Maciag, Drohan: "Human endothelial cell growth factor: cloning, nucleotide sequence, and chromosome localization." in: Science (New York, N.Y.), Vol. 233, Issue 4763, pp. 541-5, 1986

Images

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Supplier Images
ELISA image for FGF1 ELISA Kit (Fibroblast Growth Factor 1 (Acidic)) (ABIN1889350) Rat FGF1 PicoKine ELISA Kit standard curve