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|Application / Reactivity||Human|
|Blocking Antibody (Inhibition)||1 Antibodies|
|Cell Culture (CC)||1 Antibodies|
|Enzyme Immunoassay (EIA)||6 Antibodies|
|Functional Studies (Func)||2 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||1 Antibodies|
|Immunohistochemistry (IHC)||15 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||5 Antibodies|
|Immunoprecipitation (IP)||2 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Western Blotting (WB)||82 Antibodies|
|Antigen||Fibroblast Growth Factor 10 (FGF10) Antibodies|
|Conjugate||This FGF10 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Functional Studies (Func), Western Blotting (WB)
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Product Details anti-FGF10 AntibodyTarget Details FGF10 Application Details Handling Images
|Specificity||Fibroblast Growth Factor 10 (FGF-10)|
|Immunogen||Recombinant human FGF-10 (hFGF-10)|
Target Details FGF10Product Details anti-FGF10 Antibody Application Details Handling Images back to top
|Alternative Name||FGF10 (FGF10 Antibody Abstract)|
|Background||FGF10 is a heparin binding growth factor that stimulates the proliferation and activation of cells that express FGF receptors. FGF10 is mostly related to FGF-7/KGF and is expressed during development and preferentially in adult lungs.Synonyms: FGF-10, Fibroblast growth factor 10, KGF2, Keratinocyte growth factor 2|
|Research Area||Cancer, Growth Factors, Angiogenesis|
|Pathways||RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Tube Formation, Positive Regulation of Response to DNA Damage Stimulus|
Application DetailsProduct Details anti-FGF10 Antibody Target Details FGF10 Handling Images back to top
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhFGF-10 (1.5 ng/mL), a concentration of 0.02-0.035 μg/mL of this antibody is required. ELISA: (Indirect): To detect hFGF-10 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hFGF-10. (Sandwich): To detect hFGF-10 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human FGF-10 (PP1119B1 or PP1119B2) as adetection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinanthFGF-10. Western Blot: To detect hFGF-10 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hFGF-10 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions. Immunohistochemistry on frozen or paraffin sections: This antibody should be used at0.5-2.0 μg/mL depending on dection system.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-FGF10 Antibody Target Details FGF10 Application Details Images back to top
|Reconstitution||Restore in sterile water to a concentration of 0.1-1.0 mg/mL.|
|Buffer||PBS, pH 7.2 without preservatives.|