anti-Mouse (Murine) FLT3 antibody for Western Blotting

Recommended FLT3 Antibody (supplied by: Log in to see )

Antigen
Fms-Related tyrosine Kinase 3 (FLT3) Antibodies
  • FLT3
  • B230315G04
  • CD135
  • Flk-2
  • Flk2
  • Flt-3
  • Ly72
  • wmfl
  • FLK-2
  • FLK2
  • STK1
  • fms-related tyrosine kinase 3
  • FMS-like tyrosine kinase 3
  • FLT3
  • Flt3
Epitope
pTyr599
36
30
29
22
20
14
14
12
12
10
8
7
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
308
158
45
4
1
1
1
1
1
1
Host
Rabbit
259
75
26
4
1
Clonality
Polyclonal
Conjugate
This FLT3 antibody is un-conjugated
21
20
17
11
10
6
6
6
6
6
6
4
4
4
4
4
4
3
2
2
2
2
1
1
Application
ELISA, Western Blotting (WB)
175
120
99
54
54
48
22
15
11
4
3
2
2
2
1
1
1
Options
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Catalog No. ABIN1531319
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.3140206 ABIN1532044 ELISA WB Rabbit IgG pTyr842 Log in to see Polyclonal 2
3.3140206 ABIN1532338 ELISA WB Rabbit IgG Log in to see Polyclonal 2
3.3140206 ABIN1077372 IF ELISA WB Rabbit IgG N-Term Log in to see Polyclonal
3.3140206 ABIN658542 WB Rabbit Ig AA 154-181, N-Term Log in to see Polyclonal
3.3140206 ABIN3184641 ELISA WB Rabbit IgG Ser387 Log in to see Polyclonal
3.3140206 ABIN3182315 ELISA WB Rabbit IgG pTyr842 Log in to see Polyclonal
3.3140206 ABIN3181448 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
3.3140206 ABIN2650914 WB Rabbit IgG Log in to see Polyclonal
3.3140206 ABIN2736759 IHC WB Rabbit IgG Log in to see Polyclonal
3.3140206 ABIN3020640 WB Rabbit IgG Log in to see Polyclonal
3.3140206 ABIN343645 WB Rabbit IgG Log in to see Polyclonal
3.3140206 ABIN3028662 ELISA WB Rabbit Ig Fraction AA 154-181 Log in to see Polyclonal
3.3140206 ABIN2429279 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
3.3140206 ABIN1847596 ELISA WB Rabbit C-Term, pTyr842, pTyr845 Log in to see Polyclonal
1 ABIN5556436 EIA IF WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN2658479 BR ICC FACS WB APC Rat IgG2a kappa Log in to see A2F10 7
1 ABIN4900092 FACS WB Goat IgG AA 28-544 Log in to see Polyclonal 1
1 ABIN684853 IF (p) IHC (p) WB Rabbit IgG pTyr589, pTyr591 Log in to see Polyclonal
1 ABIN2888661 ELISA WB Rabbit IgG AA 565-614 Log in to see Polyclonal
1 ABIN410042 IHC (p) IHC WB Rat IgG2 Log in to see 12B6

Top referenced anti-FLT3 antibody for Western Blotting

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Application / Reactivity Mouse (Murine) Human
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ELISA 53 Antibodies 104 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 4 Antibodies
Flow Cytometry (FACS) 23 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 8 Antibodies
Immunofluorescence (IF) 5 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 40 Antibodies
Immunohistochemistry (IHC) 16 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Western Blotting (WB) 92 Antibodies
Cell Culture (CC) 1 Antibodies
ELISA (Capture) 1 Antibodies

General

Antigen Fms-Related tyrosine Kinase 3 (FLT3) Antibodies
Epitope pTyr599
(36), (30), (29), (22), (20), (14), (14), (12), (12), (10), (8), (7), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(308), (158), (45), (4), (1), (1), (1), (1), (1), (1)
Host Rabbit
(259), (75), (26), (4), (1)
Clonality
Polyclonal
Conjugate This FLT3 antibody is un-conjugated
(21), (20), (17), (11), (10), (6), (6), (6), (6), (6), (6), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (1), (1)
Application ELISA, Western Blotting (WB)
(175), (120), (99), (54), (54), (48), (22), (15), (11), (4), (3), (2), (2), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-FLT3 Antibody

Target Details FLT3 Application Details Handling ProductDetails: References for anti-FLT3 antibody (ABIN1531319) Images
Specificity FLT3 (Phospho-Tyr599) Antibody detects endogenous levels of FLT3 only when phosphorylated at Tyr599.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human FLT3 around the phosphorylation site of Tyr599.
Isotype IgG

Target Details FLT3

Product Details anti-FLT3 Antibody Application Details Handling ProductDetails: References for anti-FLT3 antibody (ABIN1531319) Images back to top
Antigen
Alternative Name FLT3 (FLT3 Antibody Abstract)
Background Molecular weight of target protein: 112 kDa
Synonyms: Tyrosine-protein kinase receptor FLT3, Stem cell tyrosine kinase 1, STK-1, CD135 antigen
Molecular Weight 112 kDa
Gene ID 2322
OMIM 136351
UniProt P36888
Pathways RTK Signaling

Application Details

Product Details anti-FLT3 Antibody Target Details FLT3 Handling ProductDetails: References for anti-FLT3 antibody (ABIN1531319) Images back to top
Application Notes WB: 1:500-1:1000
ELISA: 1:1000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.507590 (NCBI Gene Symbol: FLT3)

Restrictions For Research Use only

Handling

Product Details anti-FLT3 Antibody Target Details FLT3 Application Details ProductDetails: References for anti-FLT3 antibody (ABIN1531319) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

ProductDetails: References for anti-FLT3 antibody (ABIN1531319)

Product Details anti-FLT3 Antibody Target Details FLT3 Application Details Handling Images back to top
Background publications

Greenman, Stephens, Smith, Dalgliesh, Hunter, Bignell, Davies, Teague, Butler, Stevens, Edkins, OMeara, Vastrik, Schmidt, Avis, Barthorpe, Bhamra, Buck, Choudhury, Clements, Cole, Dicks, Forbes, Gray et al.: "Patterns of somatic mutation in human cancer genomes. ..." in: Nature, Vol. 446, Issue 7132, pp. 153-8, 2007

Rosnet, Schiff, Pébusque, Marchetto, Tonnelle, Toiron, Birg, Birnbaum: "Human FLT3/FLK2 gene: cDNA cloning and expression in hematopoietic cells." in: Blood, Vol. 82, Issue 4, pp. 1110-9, 1993

Images

Product Details anti-FLT3 Antibody Target Details FLT3 Application Details Handling ProductDetails: References for anti-FLT3 antibody (ABIN1531319) back to top
Supplier Images
Western Blotting (WB) image for anti-FLT3 antibody (Fms-Related tyrosine Kinase 3) (pTyr599) (ABIN1531319) Western blot analysis of extracts from COS7 cells treated with EGF 200ng/ml 30', usin...