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anti-Mouse (Murine) Galectin 3 antibody for Flow Cytometry

Recommended Galectin 3 Antibody (supplied by: Log in to see )

Antigen
Lectin, Galactoside-Binding, Soluble, 3 (LGALS3) Antibodies
  • CBP 35
  • CBP35
  • gal-3
  • Gal-3
  • GAL3
  • gal3
  • galactoside-binding
  • GALBP
  • Galectin-3
  • galectin-3
  • GALIG
  • GBP
  • L-34
  • L31
  • lectin
  • leg3
  • LGALS2
  • LGALS3
  • Mac-2
  • MAC2
  • MGC52947
  • MGC75803
  • soluble
Reactivity
Mouse (Murine)
358
177
90
14
4
3
3
2
Host
Rat
214
177
50
14
Clonality
Monoclonal
Conjugate
This Galectin 3 antibody is un-conjugated
37
22
19
15
10
10
6
6
4
4
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
319
201
146
106
80
77
46
35
32
12
9
6
6
4
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN114277
$ 346.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30411333 ABIN2663838 BR FACS IP IHC PE Mouse IgG1 kappa AA 151-251 Log in to see Gal397 4
0.30411333 ABIN2657982 FACS Alexa Fluor 647 Rat IgG2a kappa Log in to see M3-38 8
0.30411333 ABIN2657562 FACS Alexa Fluor 488 Rat IgG2a kappa Log in to see M3-38 7
0.30411333 ABIN2661262 FACS Biotin Rat IgG2a kappa Log in to see M3-38 7
0.30411333 ABIN2663839 FACS PE Rat IgG2a kappa Log in to see M3-38 7
0.30411333 ABIN2665257 FACS Rat IgG2a kappa Log in to see M3-38 7
0.30411333 ABIN2679911 FACS EFluor 660 Rat IgG2a kappa Log in to see M3-38 (M3-38) 1
0.0010480314 ABIN114276 EIA FACS IHC (fro) IHC (p) Biotin Rat IgG2a Log in to see M3-38 6
0.0010480314 ABIN1804652 IHC (fro) FACS IHC (p) ELISA WB Rat IgG2a, kappa Log in to see M3-38
0.0010480314 ABIN474396 FACS Biotin Rat IgG2a, kappa Log in to see M3-38
0.0010480314 ABIN474582 FACS PE Rat IgG2a, kappa Log in to see M3-38
0.0010480314 ABIN1101661 IHC (fro) FACS IHC (p) ELISA Rat IgG2a Log in to see M3-38
0.0010480314 ABIN2661261 FACS Biotin Rat IgG2a kappa Log in to see M3-38 8
0.0010480314 ABIN2663840 FACS PE Rat IgG2a kappa Log in to see M3-38 7
0.0010480314 ABIN280795 FACS IHC (fro) IHC (p) IP ELISA WB Rat IgG2a kappa Log in to see M3-38 (M3-38)
0.0010480314 ABIN2665256 FACS Rat IgG2a kappa Log in to see M3-38 7
0.0010480314 ABIN278853 FACS PE Rat IgG2a kappa Log in to see M3-38 (M3-38)
0.0010480314 ABIN2271833 FACS IP IHC ELISA WB Rat IgG2a kappa Log in to see M3-38
0.0010480314 ABIN393010 FACS IHC (fro) IHC (p) ELISA Biotin Rat IgG2a Log in to see M3-38
0.0010480314 ABIN393012 FACS IHC (fro) IHC (p) ELISA FITC Rat IgG2a Log in to see M3-38

Top referenced anti-Galectin 3 antibody for Flow Cytometry

Similar anti-Galectin 3 Antibodies

Application / Reactivity Mouse (Murine)
Blocking Reagent (BR) 1 Antibodies
ELISA 86 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 45 Antibodies
Functional Studies (Func) 2 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 41 Antibodies
Immunofluorescence (IF) 52 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 62 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 26 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 54 Antibodies
Immunoprecipitation (IP) 24 Antibodies
Western Blotting (WB) 137 Antibodies

General

Antigen Lectin, Galactoside-Binding, Soluble, 3 (LGALS3) Antibodies
Reactivity Mouse (Murine)
(358), (177), (90), (14), (4), (3), (3), (2)
Host Rat
(214), (177), (50), (14)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Galectin 3 antibody is un-conjugated
(37), (22), (19), (15), (10), (10), (6), (6), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(319), (201), (146), (106), (80), (77), (46), (35), (32), (12), (9), (6), (6), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 12 references available
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Product Details anti-Galectin 3 Antibody

Target Details Galectin 3 Application Details Handling References for anti-Galectin 3 Antibody (ABIN114277) Images
Cross-Reactivity (Details) Species reactivity (tested):Mouse.
Purification Protein G Chromatography.
Immunogen Plasma membrane glycoproteins from C57BL/6 mouse thioglycollate-elicited peritoneal exudate
Clone M3-38
Isotype IgG2a

Target Details Galectin 3

Product Details anti-Galectin 3 Antibody Application Details Handling References for anti-Galectin 3 Antibody (ABIN114277) Images back to top
Antigen
Alternative Name Galectin-3 (LGALS3 Antibody Abstract)
Background Galectins are a new family of animal lectins which appear to exhibit a variety of biological functions. Lectins, of either plant or animal origin, are carbohydrate binding proteins that interact with glycoprotein and glycolipids on the surface of animal cells. The Galectins are lectins that recognize and interact with beta-galactoside moieties. Galectin 3 is one of the more extensively studied members of this family and is a 30 kDa protein. Due to a C-terminal carbohydrate binding site, Galectin 3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homooligomerized. Galectin 3 is normally distributed in epithelia of many organs, in various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins.Synonyms: 35 kDa lectin, CBP35, Carbohydrate-binding protein 35, GAL3, GALBP, Galactose-specific lectin 3, IgE-binding protein, L-31, L-34 galactoside-binding lectin, Laminin-binding protein, Lectin L-29, Lgals3, Mac-2
Gene ID 10090
UniProt P16110
Research Area Neurology
Pathways RTK Signaling

Application Details

Product Details anti-Galectin 3 Antibody Target Details Galectin 3 Handling References for anti-Galectin 3 Antibody (ABIN114277) Images back to top
Application Notes Suitable for use in Indirect Immunofluorescence staining, including Flow Cytometricanalysis of live cells. The addition of Propidium Iodide is optional, its use eliminatesstaining artefacts caused by dead cells (1). This antibody is also suitable for Frozen Sections (4,5), Paraffin Sections (7) and ELISA (6).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Flow Cytometry Analysis: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 0. 5-1. 0 µg of antibody per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody FITC Goat anti-rat IgG (H+L) at appropriate dilution. 9. Incubate the tubes at 4°C for 30-60 minutes. (It is recommended that the tubes are protected from light since most fluorochromes arelight sensitive). 10. Wash 2 times at 4°C in media B. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls).
Restrictions For Research Use only

Handling

Product Details anti-Galectin 3 Antibody Target Details Galectin 3 Application Details References for anti-Galectin 3 Antibody (ABIN114277) Images back to top
Concentration 1.0 mg/mL
Buffer PBS with 0.02 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Galectin 3 Antibody (ABIN114277)

Product Details anti-Galectin 3 Antibody Target Details Galectin 3 Application Details Handling Images back to top
Product cited in:

Jindra, Hsueh, Hong, Gjertson, Shen, Gao, Dang, Mischel, Baldwin, Fishbein, Kupiec-Weglinski, Reed: "Anti-MHC class I antibody activation of proliferation and survival signaling in murine cardiac allografts." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 4, pp. 2214-24, 2008

Background publications

Kowala, Recce, Beyer, Gu, Valentine: "Characterization of atherosclerosis in LDL receptor knockout mice: macrophage accumulation correlates with rapid and sustained expression of aortic MCP-1/JE." in: Atherosclerosis, Vol. 149, Issue 2, pp. 323-30, 2000

Saada, Reichert, Rotshenker: "Granulocyte macrophage colony stimulating factor produced in lesioned peripheral nerves induces the up-regulation of cell surface expression of MAC-2 by macrophages and Schwann cells." in: The Journal of cell biology, Vol. 133, Issue 1, pp. 159-67, 1996

Mey, Leffler, Hmama, Normier, Revillard: "The animal lectin galectin-3 interacts with bacterial lipopolysaccharides via two independent sites." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 4, pp. 1572-7, 1996

Cua, Hinton, Kirkman, Stohlman: "Macrophages regulate induction of delayed-type hypersensitivity and experimental allergic encephalomyelitis in SJL mice." in: European journal of immunology, Vol. 25, Issue 8, pp. 2318-24, 1995

Knisley, Weitlauf: "Compartmentalized reactivity of M3/38 (anti-Mac-2) and M3/84 (anti-Mac-3) in the uterus of pregnant mice." in: Journal of reproduction and fertility, Vol. 97, Issue 2, pp. 521-7, 1993

Weitlauf, Knisley: "Changes in surface antigens on preimplantation mouse embryos." in: Biology of reproduction, Vol. 46, Issue 5, pp. 811-6, 1992

Huang, Meyer, Kubai, Auerbach: "An immune model of beryllium-induced pulmonary granulomata in mice. Histopathology, immune reactivity, and flow-cytometric analysis of bronchoalveolar lavage-derived cells." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 67, Issue 1, pp. 138-46, 1992

Sasaki, Dumas, Engleman: "Discrimination of viable and non-viable cells using propidium iodide in two color immunofluorescence." in: Cytometry, Vol. 8, Issue 4, pp. 413-20, 1987

Leenen, Jansen, van Ewijk: "Murine macrophage cell lines can be ordered in a linear differentiation sequence." in: Differentiation; research in biological diversity, Vol. 32, Issue 2, pp. 157-64, 1987

Ho, Springer: "Preparation and use of monoclonal antimacrophage antibodies." in: Methods in enzymology, Vol. 108, pp. 313-24, 1985

Springer: "Monoclonal antibody analysis of complex biological systems. Combination of cell hybridization and immunoadsorbents in a novel cascade procedure and its application to the macrophage cell surface." in: The Journal of biological chemistry, Vol. 256, Issue 8, pp. 3833-9, 1981

Images

Product Details anti-Galectin 3 Antibody Target Details Galectin 3 Application Details Handling References for anti-Galectin 3 Antibody (ABIN114277) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Galectin 3 antibody (Lectin, Galactoside-Binding, Soluble, 3) (ABIN114277) Cell source: Thioglycollate-elicited peritonperitoneal exudate. Percentage of cells s...