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anti-Human HER2 antibody for Intracellular Flow Cytometry

Recommended HER2 Antibody (supplied by: Log in to see )

Antigen
V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
  • c-erbB2
  • c-neu
  • CD340
  • Erbb-2
  • HER-2
  • HER-2/neu
  • HER2
  • mKIAA3023
  • MLN 19
  • NEU
  • Neu
  • NGL
  • TKR1
Reactivity
Human
1322
418
261
24
13
4
3
3
3
3
2
2
1
1
1
Host
Mouse
969
495
25
18
5
5
Clonality
Monoclonal
Conjugate
This HER2 antibody is conjugated to FITC
74
64
60
52
45
41
17
17
10
10
9
8
8
8
8
8
8
7
7
7
7
7
7
7
7
7
7
2
2
2
Application
Functional Studies (Func), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Intracellular Flow Cytometry (ICFC), Immunoprecipitation (IP), Western Blotting (WB)
680
574
331
323
301
280
235
173
104
90
85
21
17
6
6
5
5
4
3
2
2
2
1
1
1
1
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Catalog No. ABIN2661687
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008737065 ABIN2658634 FACS ICFC APC Mouse IgG1 kappa Log in to see 24D2 6
0.0008737065 ABIN2658635 FACS ICFC APC Mouse IgG1 kappa Log in to see 24D2 6

Top referenced anti-HER2 antibody for Intracellular Flow Cytometry

Similar anti-HER2 Antibodies

Application / Reactivity Human
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 192 Antibodies
ELISA 439 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 17 Antibodies
Flow Cytometry (FACS) 275 Antibodies
Functional Studies (Func) 5 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 5 Antibodies
Immunocytochemistry (ICC) 86 Antibodies
Immunoelectron Microscopy (IEM) 6 Antibodies
Immunofluorescence (IF) 302 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 90 Antibodies
Immunohistochemistry (IHC) 329 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 5 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 2 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 104 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 235 Antibodies

General

Antigen V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
Reactivity Human
(1322), (418), (261), (24), (13), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1)
Host Mouse
(969), (495), (25), (18), (5), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HER2 antibody is conjugated to FITC
(74), (64), (60), (52), (45), (41), (17), (17), (10), (10), (9), (8), (8), (8), (8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (2), (2), (2)
Application Functional Studies (Func), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Intracellular Flow Cytometry (ICFC), Immunoprecipitation (IP), Western Blotting (WB)
(680), (574), (331), (323), (301), (280), (235), (173), (104), (90), (85), (21), (17), (6), (6), (5), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-HER2 Antibody

Target Details HER2 Application Details Handling References for anti-HER2 Antibody (ABIN2661687) Images
Purification The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Clone 24D2
Isotype IgG1 kappa

Target Details HER2

Product Details anti-HER2 Antibody Application Details Handling References for anti-HER2 Antibody (ABIN2661687) Images back to top
Antigen
Alternative Name CD340 (ERBB2 Antibody Abstract)
Background CD340 is also known as HER2/erbB2, tyrosine kinase cell surface receptor HER2, oncogene ERBB2, and oncogene NGL, neuroblastoma- or glioblastoma-derived. It is a member of the epidermal growth factor receptor family of cell membrane tyrosine kinases containing a single transmembrane domain and has an approximate molecular weight of 185 kD. CD340 contains three furin repeats and two cheY homologous receiver domains in the extracellular region (ECR). The HER2/erbB2 protein is expressed on many tumor cells, including some breast cancers, lung adenocarcinoma, gastric cancer, ovarian cancer, gliblastoma, some c-ALL blasts and bone marrow mesenchymal stem cells. Although the erbB2/HER2 protein contains no ligand binding domain, this protein interacts with other EGF receptor family members to form a heterodimer, stabilize ligand binding, and enhance kinase-mediated downstream signaling. CD340 has been shown to be involved in embryonic development and cancer progression, this protein is amplified in adenocarcinoma of the lung, some breast cancers, glioblastoma, gastric cancer, and ovarian carcinoma, where it functions as an oncogene. Overexpression of erbB2 in breast cancers has been shown to confer Taxol resistance and is a therapeutic target in a number of human cancers. The erbB2/HER-2 protein has been shown to interact with a large number of proteins, including SHC1, EGF receptorm, neuroregulin 1, c-Src, integrin β4, Grb2, Grb7, SOS1, caveolin 1, JAK2, PAK1, FAK, and β-catenin, among others. CD340 is extensively modified by tyrosine phosphoryation on multiple residues (Y1023, Y1139, Y1196, Y1221, Y1222, Y1248).
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-HER2 Antibody Target Details HER2 Handling References for anti-HER2 Antibody (ABIN2661687) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-HER2 Antibody Target Details HER2 Application Details References for anti-HER2 Antibody (ABIN2661687) Images back to top
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-HER2 Antibody (ABIN2661687)

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling Images back to top
Background publications

Leone, Perissinotto, Cavalloni, Fonsato, Bruno, Surrenti, Hong, Capaldi, Geuna, Piacibello, Aglietta: "Expression of the c-ErbB-2/HER2 proto-oncogene in normal hematopoietic cells." in: Journal of leukocyte biology, Vol. 74, Issue 4, pp. 593-601, 2003

Pegram, Finn, Arzoo, Beryt, Pietras, Slamon: "The effect of HER-2/neu overexpression on chemotherapeutic drug sensitivity in human breast and ovarian cancer cells." in: Oncogene, Vol. 15, Issue 5, pp. 537-47, 1997

Bühring, Sures, Jallal, Weiss, Busch, Ludwig, Handgretinger, Waller, Ullrich: "The receptor tyrosine kinase p185HER2 is expressed on a subset of B-lymphoid blasts from patients with acute lymphoblastic leukemia and chronic myelogenous leukemia." in: Blood, Vol. 86, Issue 5, pp. 1916-23, 1995

Slamon, Godolphin, Jones, Holt, Wong, Keith, Levin, Stuart, Udove, Ullrich: "Studies of the HER-2/neu proto-oncogene in human breast and ovarian cancer." in: Science (New York, N.Y.), Vol. 244, Issue 4905, pp. 707-12, 1989

Akiyama, Sudo, Ogawara, Toyoshima, Yamamoto: "The product of the human c-erbB-2 gene: a 185-kilodalton glycoprotein with tyrosine kinase activity." in: Science (New York, N.Y.), Vol. 232, Issue 4758, pp. 1644-6, 1986

Bargmann, Hung, Weinberg: "The neu oncogene encodes an epidermal growth factor receptor-related protein." in: Nature, Vol. 319, Issue 6050, pp. 226-30, 1986

Images

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling References for anti-HER2 Antibody (ABIN2661687) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-HER2 antibody (V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian))  (FITC) (ABIN2661687) anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) antibody (FITC)