anti-Pig (Porcine) HER2 antibody for Western Blotting

Recommended HER2 Antibody (supplied by: Log in to see )

Antigen
V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
  • CD340
  • HER-2
  • HER-2/neu
  • HER2
  • MLN 19
  • NEU
  • NGL
  • TKR1
  • Erbb-2
  • Neu
  • c-erbB2
  • c-neu
  • mKIAA3023
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog
  • v-erb-b2 avian erythroblastic leukemia viral oncogene 2
  • v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
  • ERBB2
  • Erbb2
Epitope
AA 1243-1255
104
82
72
70
56
54
53
48
46
35
26
24
22
22
20
20
20
20
20
20
20
20
20
19
19
19
19
18
17
15
13
13
13
12
11
8
8
8
8
8
7
7
6
6
5
5
5
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus)
1348
495
329
35
33
15
12
7
5
5
4
3
3
3
2
1
1
1
1
1
Host
Rabbit
980
511
30
16
12
5
1
Clonality
Polyclonal
Conjugate
This HER2 antibody is un-conjugated
76
66
59
54
46
41
19
19
12
12
11
10
10
9
8
8
8
8
8
7
7
7
7
7
7
7
7
6
3
2
2
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
701
596
311
310
309
297
247
137
109
102
89
21
19
14
8
6
6
5
5
3
3
3
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN305740
$ 451.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2470582 IP IHC WB Rabbit IgG AA 1243-1255 Log in to see Polyclonal
1 ABIN576254 IHC (fro) ELISA WB Rabbit IgG pThr686 Log in to see Polyclonal
1 ABIN1893701 IF IP IHC (p) WB Rabbit IgG AA 975-1025 Log in to see Polyclonal
1 ABIN1893702 IF IP IHC (p) PLA WB Rabbit IgG AA 1200-1255, C-Term Log in to see Polyclonal

Similar anti-HER2 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Dog (Canine) Monkey Hamster Horse (Equine) Pig (Porcine)
Immunofluorescence (fixed cells) (IF/ICC) 8 Antibodies 2 Antibodies 2 Antibodies 1 Antibodies
Cytometry by Time of Flight (CyTOF) 14 Antibodies 2 Antibodies
Western Blotting (WB) 669 Antibodies 27 Antibodies 248 Antibodies 225 Antibodies 28 Antibodies 6 Antibodies 8 Antibodies 5 Antibodies
Radioimmunoassay (RIA) 2 Antibodies
Proximity Ligation Assay (PLA) 3 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies

General

Antigen V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
Epitope AA 1243-1255
(104), (82), (72), (70), (56), (54), (53), (48), (46), (35), (26), (24), (22), (22), (20), (20), (20), (20), (20), (20), (20), (20), (20), (19), (19), (19), (19), (18), (17), (15), (13), (13), (13), (12), (11), (8), (8), (8), (8), (8), (7), (7), (6), (6), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(1348), (495), (329), (35), (33), (15), (12), (7), (5), (5), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Host Rabbit
(980), (511), (30), (16), (12), (5), (1)
Clonality
Polyclonal
Conjugate This HER2 antibody is un-conjugated
(76), (66), (59), (54), (46), (41), (19), (19), (12), (12), (11), (10), (10), (9), (8), (8), (8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (6), (3), (2), (2)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(701), (596), (311), (310), (309), (297), (247), (137), (109), (102), (89), (21), (19), (14), (8), (6), (6), (5), (5), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-HER2 Antibody

Target Details HER2 Application Details Handling Images
Brand IHC-plus™
Specificity Recognizes c-erbB-2 (185kD), but not c-erbB-1(EGF-R), c-erbB-3 or c-erbB-4.
Predicted Reactivity Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Horse, Pig (100%) Elephant, Bat, Bovine (92%) Opossum (85%).
Purification Protein A purified
Immunogen A KLH-linked peptide (AENPEYLGLDVPV) corresponding to aa1243-1255 from the human c-erbB-2/HER-2/neu oncoprotein. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Horse, Pig (100%), Elephant, Bat, Bovine (92%), Opossum (85%).
Isotype IgG

Target Details HER2

Product Details anti-HER2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name ERBB2 / HER2 (ERBB2 Antibody Abstract)
Background Name/Gene ID: ERBB2
Subfamily: EGF Receptor
Family: Protein Kinase

Synonyms: ERBB2, C-erb B2/neu protein, CD340, HER2, Herstatin, MLN 19, HER-2, Neu, NGL, Proto-oncogene c-ErbB-2, Proto-oncogene Neu, CD340 antigen, MLN19, p185erbB2, TKR1
Gene ID 2064
UniProt P04626
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development

Application Details

Product Details anti-HER2 Antibody Target Details HER2 Handling Images back to top
Application Notes Approved: IHC-Fr, IHC-P (5 μg/mL), IP, WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 0.5-2 μg/mL detects c-erbB-2 in RIPA lysates from A431, MDA, MCF-7, LNCap, and c-erbB-2 transfected 3T3/NIH cells. Immunoprecipitation: 4 μg immunoprecipitated c-erbB-2 from 500 μg of A431, LNCap, and c-erbB- 2 transfected 3T3/NIH RIPA lysate.
Restrictions For Research Use only

Handling

Product Details anti-HER2 Antibody Target Details HER2 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer 10 mM PBS, pH 7.4, 0.2 % BSA, 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.

Images

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-HER2 antibody (V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian)) (AA 1243-1255) (ABIN305740) Human Breast, Carcinoma (formalin-fixed, paraffin-embedded) stained with ERBB2 antibo...