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anti-Human KIT Ligand antibody for Functional Studies

Recommended KIT Ligand Antibody (supplied by: Log in to see )

Antigen
KIT Ligand (KITLG) Antibodies
  • 710-712
  • blz
  • Clo
  • Con
  • contrasted
  • CSF
  • FPH2
  • Gb
  • Kitl
  • kitl
  • KITL
  • Kitlg
  • KITLG
  • KL-1
  • kl-1
  • Mgf
  • MGF
  • mgf
  • scf
  • SCF
  • SF
  • SHEP7
  • Sl
  • SLF
  • steel
  • Xkl-1
  • Xsl
  • Xsl-1
  • Xsl-2
Reactivity
Human
182
68
59
4
1
1
1
1
1
Host
Rabbit
188
45
22
1
Clonality
Polyclonal
Conjugate
This KIT Ligand antibody is un-conjugated
36
11
8
8
7
7
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
211
151
37
34
25
20
20
16
11
9
9
9
6
4
1
1
1
1
1
Supplier
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Catalog No. ABIN116225
$ 265.65
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008758347 ABIN116226 EIA Func IHC (p) WB Rabbit Log in to see Polyclonal
0.0008758347 ABIN2476604 Func IHC (p) ELISA WB Goat IgG Log in to see Polyclonal 2
0.0008758347 ABIN641063 Func ELISA WB Goat Log in to see

Top referenced anti-KIT Ligand antibody for Functional Studies

Similar anti-KIT Ligand Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 5 Antibodies
ELISA 103 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 11 Antibodies
Flow Cytometry (FACS) 21 Antibodies
Functional Studies (Func) 4 Antibodies
Immunocytochemistry (ICC) 7 Antibodies
Immunofluorescence (IF) 16 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 36 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 25 Antibodies
Neutralization (Neut) 15 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Radioimmunoassay (RIA) 2 Antibodies
Western Blotting (WB) 149 Antibodies

General

Antigen KIT Ligand (KITLG) Antibodies
Reactivity Human
(182), (68), (59), (4), (1), (1), (1), (1), (1)
Host Rabbit
(188), (45), (22), (1)
Clonality
Polyclonal
Conjugate This KIT Ligand antibody is un-conjugated
(36), (11), (8), (8), (7), (7), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(211), (151), (37), (34), (25), (20), (20), (16), (11), (9), (9), (9), (6), (4), (1), (1), (1), (1), (1)
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Product Details anti-KIT Ligand Antibody

Target Details KIT Ligand Application Details Handling Images
Specificity Human Stem Cell Factor (SCF).
Purification Affinity chromatography.
Immunogen Highly pure (>98%) recombinant hSCF (anti-hSCF).

Target Details KIT Ligand

Product Details anti-KIT Ligand Antibody Application Details Handling Images back to top
Antigen
Alternative Name KITLG / SCF (KITLG Antibody Abstract)
Background Synonyms: KITL, Kit ligand, MGF, Mast cell growth factor, Stem cell factor, c-Kit ligand
Gene ID 4254
NCBI Accession NP_000890
UniProt P21583
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-KIT Ligand Antibody Target Details KIT Ligand Handling Images back to top
Application Notes Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhSCF (10 ng/mL), a concentration of 8 - 12 ng/mL of this antibody is required. ELISA: To detect hSCF by direct ELISA (using 100 l/well antibody solution) a concentrationof at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/wellof recombinant hSCF. Western Blot: To detect hSCF by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hSCF is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions. Immunohistochemistry: 1 μg/mL is the recommended starting dilution for frozen andparaffin sections. For paraffin sections antigen retrieval techniques may be necesserary toimprove staining.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-KIT Ligand Antibody Target Details KIT Ligand Application Details Images back to top
Reconstitution Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer PBS, pH 7.2 without preservatives.
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
Storage 4 °C/-20 °C
Storage Comment Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.