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|Application / Reactivity||Human|
|Biochemical Assay (BCA)||1 Antibodies|
|Blocking Antibody (Inhibition)||5 Antibodies|
|ELISA (Capture)||1 Antibodies|
|Enzyme Immunoassay (EIA)||11 Antibodies|
|Flow Cytometry (FACS)||21 Antibodies|
|Functional Studies (Func)||4 Antibodies|
|Immunocytochemistry (ICC)||6 Antibodies|
|Immunofluorescence (IF)||15 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||33 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||4 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||23 Antibodies|
|Neutralization (Neut)||14 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Radioimmunoassay (RIA)||2 Antibodies|
|Western Blotting (WB)||142 Antibodies|
|Antigen||KIT Ligand (KITLG) Antibodies|
|Conjugate||This KIT Ligand antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
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Product Details anti-KIT Ligand AntibodyTarget Details KIT Ligand Application Details Handling Images
|Purification||Tissue culture supernatant|
|Immunogen||A synthetic peptide corresponding to residues near the N-terminus of human SCF was used as an immunogen.|
Target Details KIT LigandProduct Details anti-KIT Ligand Antibody Application Details Handling Images back to top
|Alternative Name||KITLG / SCF (KITLG Antibody Abstract)|
Novel hematopoietic growth factor, stem cell factor (SCF), was cloned anddemonstrated to be the ligand for the c-kit tyrosine kinase receptor. SCF is encoded bySl (steel), a gene critical to the development of several distinct cell lineages duringembryonic life and which has important effects on hematopoiesis in the adult (1). SCF isable to augment the proliferation of both myeloid and lymphoid hematopoieticprogenitors in bone marrow cultures. SCF exhibits potent synergistic activities inconjunction with colony-stimulating factors, resulting in increased colony numbers andcolony size (2). Alternate Names: anti-C kit ligand antibody, anti-Ckit ligand antibody, anti--DKFZp686F2250 antibody,anti-KIT ligand antibody, anti-Kitl antibody, anti-KITLG antibody, anti-KL 1 antibody,anti-KL1 antibody, anti-Mast cell growth factor antibody, anti-MGF antibody, anti-SFantibody, anti-Steel factor antibody, anti-Stem cell factor antibody, anti-Stem cell factorprecursor antibody.
Gene Symbol: KITLG
|Gene ID||4254, 17311, 60427|
|Pathways||RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-KIT Ligand Antibody Target Details KIT Ligand Handling Images back to top
In Western blot this antibody detects a band at approximately 18kDa.
Recommended dilutions: Flow Cytometry 1:90, Immunocytochemistry/Immunofluorescence 1:100-250, Immunohistochemistry 1:100-250, Immunohistochemistry-Paraffin 1:100-250, Western Blot 1:1000-10000
Protocol specific for SCF Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
|Restrictions||For Research Use only|
HandlingProduct Details anti-KIT Ligand Antibody Target Details KIT Ligand Application Details Images back to top
|Buffer||PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage Comment||-20 °C.|
ImagesProduct Details anti-KIT Ligand Antibody Target Details KIT Ligand Application Details Handling back to top