You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human KIT Ligand antibody for Immunocytochemistry

Recommended KIT Ligand Antibody (supplied by: Log in to see )

Antigen
KIT Ligand (KITLG) Antibodies
  • 710-712
  • blz
  • Clo
  • Con
  • contrasted
  • CSF
  • FPH2
  • Gb
  • Kitl
  • kitl
  • KITL
  • Kitlg
  • KITLG
  • KL-1
  • kl-1
  • Mgf
  • MGF
  • mgf
  • scf
  • SCF
  • SF
  • SHEP7
  • Sl
  • SLF
  • steel
  • Xkl-1
  • Xsl
  • Xsl-1
  • Xsl-2
Epitope
N-Term
21
14
9
8
7
7
5
4
4
3
3
3
3
3
3
2
2
2
2
1
1
1
1
Reactivity
Human
176
67
58
4
1
1
1
1
1
Host
Rabbit
184
43
22
1
Clonality
Monoclonal
Conjugate
This KIT Ligand antibody is un-conjugated
36
11
8
8
7
7
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
205
149
34
33
24
21
20
15
11
10
9
8
6
4
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN258461
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010215953 ABIN1908272 ICC FACS ELISA WB PE Rabbit IgG C-Term Log in to see Polyclonal
0.0010215953 ABIN1908270 ICC FACS ELISA WB APC Rabbit IgG C-Term Log in to see Polyclonal
0.0010215953 ABIN1908269 ICC FACS ELISA WB Alkaline Phosphatase (AP) Rabbit IgG C-Term Log in to see Polyclonal
0.0010215953 ABIN1908271 ICC FACS ELISA WB FITC Rabbit IgG C-Term Log in to see Polyclonal
0.0010215953 ABIN1908268 ICC FACS ELISA WB HRP Rabbit IgG C-Term Log in to see Polyclonal

Similar anti-KIT Ligand Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 5 Antibodies
ELISA 101 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 11 Antibodies
Flow Cytometry (FACS) 21 Antibodies
Functional Studies (Func) 4 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 15 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 33 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 23 Antibodies
Neutralization (Neut) 14 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Radioimmunoassay (RIA) 2 Antibodies
Western Blotting (WB) 142 Antibodies

General

Antigen KIT Ligand (KITLG) Antibodies
Epitope N-Term
(21), (14), (9), (8), (7), (7), (5), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human
(176), (67), (58), (4), (1), (1), (1), (1), (1)
Host Rabbit
(184), (43), (22), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This KIT Ligand antibody is un-conjugated
(36), (11), (8), (8), (7), (7), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(205), (149), (34), (33), (24), (21), (20), (15), (11), (10), (9), (8), (6), (4), (1), (1), (1), (1)
Supplier Log in to see

Product Details anti-KIT Ligand Antibody

Target Details KIT Ligand Application Details Handling Images
Purification Tissue culture supernatant
Immunogen A synthetic peptide corresponding to residues near the N-terminus of human SCF was used as an immunogen.
Clone EP665Y
Isotype IgG

Target Details KIT Ligand

Product Details anti-KIT Ligand Antibody Application Details Handling Images back to top
Antigen
Alternative Name KITLG / SCF (KITLG Antibody Abstract)
Background Novel hematopoietic growth factor, stem cell factor (SCF), was cloned anddemonstrated to be the ligand for the c-kit tyrosine kinase receptor. SCF is encoded bySl (steel), a gene critical to the development of several distinct cell lineages duringembryonic life and which has important effects on hematopoiesis in the adult (1). SCF isable to augment the proliferation of both myeloid and lymphoid hematopoieticprogenitors in bone marrow cultures. SCF exhibits potent synergistic activities inconjunction with colony-stimulating factors, resulting in increased colony numbers andcolony size (2). Alternate Names: anti-C kit ligand antibody, anti-Ckit ligand antibody, anti--DKFZp686F2250 antibody,anti-KIT ligand antibody, anti-Kitl antibody, anti-KITLG antibody, anti-KL 1 antibody,anti-KL1 antibody, anti-Mast cell growth factor antibody, anti-MGF antibody, anti-SFantibody, anti-Steel factor antibody, anti-Stem cell factor antibody, anti-Stem cell factorprecursor antibody.
Gene Symbol: KITLG
Gene ID 4254, 17311, 60427
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-KIT Ligand Antibody Target Details KIT Ligand Handling Images back to top
Application Notes In Western blot this antibody detects a band at approximately 18kDa.
Recommended dilutions: Flow Cytometry 1:90, Immunocytochemistry/Immunofluorescence 1:100-250, Immunohistochemistry 1:100-250, Immunohistochemistry-Paraffin 1:100-250, Western Blot 1:1000-10000
Protocol Protocol specific for SCF Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only

Handling

Product Details anti-KIT Ligand Antibody Target Details KIT Ligand Application Details Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-KIT Ligand Antibody Target Details KIT Ligand Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-KIT Ligand antibody (KITLG) (N-Term) (ABIN258461) Western blot analysis on 5ng of recombinant protein using SCF RabMAb (cat. # 110-5747...
Immunohistochemistry (IHC) image for anti-KIT Ligand antibody (KITLG) (N-Term) (ABIN258461) Immunohistochemical analysis of paraffin-embedded human klidney carcinoma using anti-...