anti-Mouse (Murine) Neuregulin 1 antibody for Immunohistochemistry (Frozen Sections)

Recommended Neuregulin 1 Antibody (supplied by: Log in to see )

Antigen
Neuregulin 1 (NRG1) Antibodies
  • NRG1
  • wu:fm70e01
  • DKFZp459A1838
  • HPRG
  • HRGP
  • THPH11
  • HRG1
  • ARIA
  • GGF
  • GGF2
  • HGL
  • HRG
  • HRGA
  • MST131
  • NDF
  • SMDF
  • 6030402G23Rik
  • D230005F13Rik
  • GGFII
  • HRGalpha
  • Hgl
  • pro-neuregulin-1
  • NRG-1
  • heregulin
  • nrg1-A
  • neuregulin 1
  • histidine-rich glycoprotein
  • NRG1
  • nrg1
  • Nrg1
  • HRG
  • Hrg
Epitope
Extracellular Domain
23
20
16
14
11
10
8
7
7
7
6
6
5
5
4
4
4
4
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
239
80
76
3
3
2
1
1
1
1
1
Host
Mouse
204
68
Clonality
Monoclonal
Conjugate
This Neuregulin 1 antibody is un-conjugated
21
13
7
4
4
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
225
128
69
44
19
16
14
11
8
5
5
5
4
4
3
1
Supplier
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Catalog No. ABIN264425
$ 654.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN3031973 ICC IHC (fro) IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal

General

Antigen Neuregulin 1 (NRG1) Antibodies
Epitope Extracellular Domain
(23), (20), (16), (14), (11), (10), (8), (7), (7), (7), (6), (6), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(239), (80), (76), (3), (3), (2), (1), (1), (1), (1), (1)
Host Mouse
(204), (68)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Neuregulin 1 antibody is un-conjugated
(21), (13), (7), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
(225), (128), (69), (44), (19), (16), (14), (11), (8), (5), (5), (5), (4), (4), (3), (1)
Pubmed 4 references available
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Product Details anti-Neuregulin 1 Antibody

Target Details Neuregulin 1 Application Details Handling References for anti-Neuregulin 1 antibody (ABIN264425) Images
Specificity Recognizes a glycoprotein of 44 kDa, identified as heregulin (HRG) or neu differentiation factor (NDF) which binds to c-erbB-3 and c-erbB-4 receptors with low and high affinities, respectively. This antibody is directed against the Extracellular domain of NDF/HRG and is highly specific. It shows no cross-reaction with EGF and reacts with both alpha- and beta-isoforms of NDF/HRG, suggesting that its epitope is outside the EGF-domain. This antibody does not inhibit the binding of HRG to ErbB receptors and is excellent for multiple applications.Extracellular domain
Characteristics Synonyms: NRG1, GGF, HGL, HRGA, NDF, SMDF, Heregulin, HRG, Breast cancer cell differentiation factorp45, Glial growth factor, Neu differentiation factor, Pro-neuregulin-1 membrane-boundisoform, Acetylcholine receptor-inducing activity, Sensory and motor neuron-derived factor
Purification Purified
Immunogen Hybridoma produced by the fusion of spelocytes from BALB/c mice immunized withrecombinant extracellular domain of rat NDF protein coupled to KLH and mouse myelomaNSO cells. Genename: NRG1
Clone 7D5
Isotype IgG2a

Target Details Neuregulin 1

Product Details anti-Neuregulin 1 Antibody Application Details Handling References for anti-Neuregulin 1 antibody (ABIN264425) Images back to top
Antigen
Alternative Name Neuregulin 1 (NRG1 Antibody Abstract)
Background HRG/NDF exists in several isoforms, which are classified in two groups alpha and beta.Synonyms: Acetylcholine receptor-inducing activity, Breast cancer cell differentiation factor p45, GGF, Glial growth factor, HGL, HRG, HRGA, Heregulin, NDF, NRG1, Neu differentiation factor, Pro-neuregulin-1 membrane-bound isoform, SMDF, Sensory and motor neuron-derived factor
Gene ID 3084
UniProt Q02297
Research Area Growth Factors
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation

Application Details

Product Details anti-Neuregulin 1 Antibody Target Details Neuregulin 1 Handling References for anti-Neuregulin 1 antibody (ABIN264425) Images back to top
Application Notes Western Blot (1-5 μg/mL). Immunoprecipitation (2 μg/mg of protein lysate). Immunohistochemistry on Frozen and Formalin/Paraffin Sections (1/20-1/40). Positive Control: HRGa1, HRGb1 recombinant protein or MDA-MB-231 cells, prostatecarcinoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Neuregulin 1 Antibody Target Details Neuregulin 1 Application Details References for anti-Neuregulin 1 antibody (ABIN264425) Images back to top
Buffer PBS containing 0.08 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Expiry Date 12 months

References for anti-Neuregulin 1 antibody (ABIN264425)

Product Details anti-Neuregulin 1 Antibody Target Details Neuregulin 1 Application Details Handling Images back to top
Background publications

Beerli, Graus-Porta, Woods-Cook, Chen, Yarden, Hynes: "Neu differentiation factor activation of ErbB-3 and ErbB-4 is cell specific and displays a differential requirement for ErbB-2." in: Molecular and cellular biology, Vol. 15, Issue 12, pp. 6496-505, 1996

Karunagaran, Tzahar, Beerli, Chen, Graus-Porta, Ratzkin, Seger, Hynes, Yarden: "ErbB-2 is a common auxiliary subunit of NDF and EGF receptors: implications for breast cancer." in: The EMBO journal, Vol. 15, Issue 2, pp. 254-64, 1996

Graus-Porta, Beerli, Hynes: "Single-chain antibody-mediated intracellular retention of ErbB-2 impairs Neu differentiation factor and epidermal growth factor signaling." in: Molecular and cellular biology, Vol. 15, Issue 3, pp. 1182-91, 1995

Marte, Graus-Porta, Jeschke, Fabbro, Hynes, Taverna: "NDF/heregulin activates MAP kinase and p70/p85 S6 kinase during proliferation or differentiation of mammary epithelial cells." in: Oncogene, Vol. 10, Issue 1, pp. 167-75, 1995

Images

Product Details anti-Neuregulin 1 Antibody Target Details Neuregulin 1 Application Details Handling References for anti-Neuregulin 1 antibody (ABIN264425) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Neuregulin 1 (NRG1) (Extracellular Domain) antibody (ABIN264425) anti-Neuregulin 1 (NRG1) (Extracellular Domain) antibody