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Human Neurotrophin 3 ELISA Kit

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Antigen
Neurotrophin 3 (NTF3) ELISA Kits
  • AI316846
  • AI835689
  • HDNF
  • hdnf
  • ngf-2
  • NGF-2
  • ngf2
  • NGF2
  • nt-3
  • NT-3
  • Nt3
  • NT3
  • nt3
  • Ntf-3
  • NTF3
  • Nts3
  • wu:fl98d04
Epitope
AA 139-257
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
23
19
17
7
5
5
3
3
3
2
1
1
1
Methode Type
Sandwich ELISA
Detection Range
15.6-1000 pg/mL
Minimum Detection Limit
15.6 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN411338
$ 260.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32670403 ABIN414910 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 78.13-5000 pg/mL 78.13 pg/mL Log in to see
0.32670403 ABIN1116179 Sandwich ELISA Serum, Plasma, Biological Fluids 78.13-5000 pg/mL 78.13 pg/mL Log in to see
0.32670403 ABIN2708341 Sandwich ELISA Cell Culture Supernatant, Serum 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.32670403 ABIN2114634 Cell Culture Supernatant, Serum Log in to see
0.001210097 ABIN3032971 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 4.1-3000 pg/mL 4.1 pg/mL Log in to see
0.001210097 ABIN510499 Competition ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids 250-5000 pg/mL 250 pg/mL Log in to see
0.001210097 ABIN455990 Serum, Tissue Homogenate, Biological Fluids 78-5000 pg/mL 78 pg/mL Log in to see
0.001210097 ABIN365323 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant 1.25-80 ng/mL 1.25 ng/mL Log in to see
0.001210097 ABIN625065 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine Log in to see 5
0.001210097 ABIN2685105 Sandwich ELISA Cell Culture Supernatant, Serum 15.6-1000 pg/mL 15.6 pg/mL Log in to see 3
0.001210097 ABIN1029393 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 78.125-5000 pg/mL 78.125 pg/mL Log in to see
0.001210097 ABIN1112668 Sandwich ELISA Serum, Body Fluids, Tissue Lysate, Cell Culture Supernatant 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.001210097 ABIN2042617 Sandwich ELISA 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.001210097 ABIN2685106 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum, Urine Log in to see
0.001210097 ABIN1847020 Tissue Homogenate, Cell Lysate, Biological Fluids 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.001210097 ABIN2537184 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 78.13-5000 pg/mL 78.13 pg/mL Log in to see
0.001210097 ABIN2093653 Sandwich ELISA Log in to see
0.001210097 ABIN2949877 78.125-5000 pg/mL 78.125 pg/mL Log in to see
0.001210097 ABIN2116273 Log in to see
0.001210097 ABIN2116275 15.6-1000 pg/mL 15.6 pg/mL Log in to see

Top referenced Neurotrophin 3 ELISA Kit for ELISA

Similar Neurotrophin 3 ELISA Kits

Application / Reactivity Human
ELISA 22 ELISA Kits

General

Antigen Neurotrophin 3 (NTF3) ELISA Kits
Epitope AA 139-257
Reactivity Human
Kits with alternative reactivity to:
(23), (19), (17), (7), (5), (5), (3), (3), (3), (2), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 15.6-1000 pg/mL
Minimum Detection Limit 15.6 pg/mL
Application ELISA
Pubmed 6 references available
Supplier Log in to see

Product Details Neurotrophin 3 ELISA Kit

Target details Application Details Handling References for Neurotrophin 3 Kit (ABIN411338) Images
Purpose For quantitative detection of human Neurotrophin-3 in cell culture supernates and serum.
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum
Detection Method Colorimetric
Specificity Natural and recombinant human Neurotrophin-3
Cross-Reactivity (Details) There is cross-reactivates with NT-4<2.5 % , and no detectable cross-reactivity with any other relevant proteins.
Sensitivity <2pg/mL
Components
  • 96-well plate precoated with anti- human Neurotrophin-3 antibod - 1
  • Lyophilized recombinant human Neurotrophin-3 standar - 10ng/tubex2
  • Biotinylated anti- human Neurotrophin-3 antibod - 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC - 130ul(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: sf21
Immunogen sequence: Y139-T257

Target details

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Antigen
Alternative Name NTF3 (NTF3 ELISA Kit Abstract)
Background Neurotrophin-3(NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system. NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3(Ntrk3, also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest(NC) cell proliferation in vivo. NT-3 has been mapped to human chromosome 12p and mouse chromosome 6.1
Gene ID 4908
UniProt P20783
Pathways RTK Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details Neurotrophin 3 ELISA Kit Target details Handling References for Neurotrophin 3 Kit (ABIN411338) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human Neurotrophin-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Neurotrophin-3 has been precoated onto 96-well plates. Standards(sf21, Y139-T257) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Neurotrophin-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Neurotrophin-3 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human Neurotrophin-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernatants or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Neurotrophin-3 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 93, Standard deviation: 4, CV(%): 4.3
  • Sample 2: n=16, Mean(pg/ml): 357, Standard deviation: 17.5, CV(%): 4.9
  • Sample 3: n=16, Mean(pg/ml): 657, Standard deviation: 34.2, CV(%): 5.2,
  • Sample 1: n=24, Mean(pg/ml): 146, Standard deviation: 7.88, CV(%): 5.4
  • Sample 2: n=24, Mean(pg/ml): 375, Standard deviation: 22.9, CV(%): 6.1
  • Sample 3: n=24, Mean(pg/ml): 732, Standard deviation: 47.58, CV(%): 6.5
Restrictions For Research Use only

Handling

Product Details Neurotrophin 3 ELISA Kit Target details Application Details References for Neurotrophin 3 Kit (ABIN411338) Images back to top
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for Neurotrophin 3 Kit (ABIN411338)

Product Details Neurotrophin 3 ELISA Kit Target details Application Details Handling Images back to top
Product cited in:

Wang, Li, Kong, Zhao, Yang, Chen, Yan: "Enhanced expression of neurotrophic factors in the injured spinal cord through vaccination with myelin basic protein-derived peptide pulsed dendritic cells." in: Spine, Vol. 40, Issue 2, pp. 95-101, 2015

Zhao, Wang, Peng, Ren, Zhang, Guo, Xu, Lu: "Improvement in nerve regeneration through a decellularized nerve graft by supplementation with bone marrow stromal cells in fibrin." in: Cell transplantation, Vol. 23, Issue 1, pp. 97-110, 2014

Background publications

Donovan, Hahn, Tessarollo, Hempstead: "Identification of an essential nonneuronal function of neurotrophin 3 in mammalian cardiac development." in: Nature genetics, Vol. 14, Issue 2, pp. 210-3, 1996

Tessarollo, Vogel, Palko, Reid, Parada: "Targeted mutation in the neurotrophin-3 gene results in loss of muscle sensory neurons." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 25, pp. 11844-8, 1995

Kalcheim, Carmeli, Rosenthal: "Neurotrophin 3 is a mitogen for cultured neural crest cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 5, pp. 1661-5, 1992

Ozçelik, Rosenthal, Francke: "Chromosomal mapping of brain-derived neurotrophic factor and neurotrophin-3 genes in man and mouse." in: Genomics, Vol. 10, Issue 3, pp. 569-75, 1991

Images

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Supplier Images
ELISA image for Neurotrophin 3 ELISA Kit (NTF3) (ABIN411338) Human Neurotrophin-3 PicoKine ELISA Kit standard curve