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|Application / Reactivity||Rat (Rattus)|
|ELISA||19 ELISA Kits|
|Antigen||Neurotrophin 3 (NTF3) ELISA Kits|
|Reactivity||Rat (Rattus) Alternatives|
Kits with alternative reactivity to:
|Methode Type||Sandwich ELISA|
|Detection Range||15.6-1000 pg/mL|
|Minimum Detection Limit||15.6 pg/mL|
|6 references available|
|Supplier||Log in to see|
Product Details Neurotrophin 3 ELISA KitTarget details Application Details Handling References for Neurotrophin 3 Kit (ABIN411340) Images
|Purpose||For quantitative detection of rat Neurotrophin-3 in cell culture supernates and serum.|
|Sample Type||Cell Culture Supernatant, Serum|
|Specificity||Natural and recombinant rat Neurotrophin-3|
|Cross-Reactivity (Details)||There is cross-reactivates with NT-4<2.5 % , and no detectable cross-reactivity with any other relevant proteins.|
|Material not included||Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl|
Expression system for standard: sf21
Immunogen sequence: Y139-T257
Target detailsProduct Details Neurotrophin 3 ELISA Kit Application Details Handling References for Neurotrophin 3 Kit (ABIN411340) Images back to top
|Background||Neurotrophin-3(NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system. NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3(Ntrk3, also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest(NC) cell proliferation in vivo. NT-3 has been mapped to human chromosome 12p and mouse chromosome 6.|
|Pathways||RTK Signaling, Neurotrophin Signaling Pathway|
Application DetailsProduct Details Neurotrophin 3 ELISA Kit Target details Handling References for Neurotrophin 3 Kit (ABIN411340) Images back to top
|Application Notes||Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.|
|Plate||Pre-coated,Strips (12 x 8)|
|Protocol||rat Neurotrophin-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Neurotrophin-3 has been precoated onto 96-well plates. Standards(sf21, Y139-T257) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Neurotrophin-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat Neurotrophin-3 amount of sample captured in plate.|
|Assay Procedure||Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL rat Neurotrophin-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernatants or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat Neurotrophin-3 standard solution and each sample be measured in duplicate.|
|Restrictions||For Research Use only|
HandlingProduct Details Neurotrophin 3 ELISA Kit Target details Application Details References for Neurotrophin 3 Kit (ABIN411340) Images back to top
|Storage||-20 °C,4 °C|
|Storage Comment||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles|
|Expiry Date||12 months|
References for Neurotrophin 3 Kit (ABIN411340)Product Details Neurotrophin 3 ELISA Kit Target details Application Details Handling Images back to top
|Product cited in:||
Wang, Li, Kong et al.: "Enhanced expression of neurotrophic factors in the injured spinal cord through vaccination with myelin basic protein-derived peptide pulsed dendritic cells." in: Spine, Vol. 40, Issue 2, pp. 95-101, 2015 (PubMed).
Zhao, Wang, Peng et al.: "Improvement in nerve regeneration through a decellularized nerve graft by supplementation with bone marrow stromal cells in fibrin." in: Cell transplantation, Vol. 23, Issue 1, pp. 97-110, 2014 (PubMed).
Donovan, Hahn, Tessarollo et al.: "Identification of an essential nonneuronal function of neurotrophin 3 in mammalian cardiac development." in: Nature genetics, Vol. 14, Issue 2, pp. 210-3, 1996 (PubMed).
Tessarollo, Vogel, Palko et al.: "Targeted mutation in the neurotrophin-3 gene results in loss of muscle sensory neurons." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 25, pp. 11844-8, 1995 (PubMed).
Ozçelik, Rosenthal, Francke: "Chromosomal mapping of brain-derived neurotrophic factor and neurotrophin-3 genes in man and mouse." in: Genomics, Vol. 10, Issue 3, pp. 569-75, 1991 (PubMed).
Kalcheim, Carmeli, Rosenthal: "Neurotrophin 3 is a mitogen for cultured neural crest cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 5, pp. 1661-5, 1992 (PubMed).
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