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anti-Mouse (Murine) NFAT1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended NFAT1 Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1) Antibodies
  • NFATC2
  • AI607462
  • NF-ATc2
  • NFAT1
  • NFAT1-D
  • Nfatp
  • NFATP
  • nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2
  • nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 2
  • NFATC2
  • nfatc2
  • Nfatc2
Reactivity
Human, Mouse (Murine), Rat (Rattus)
146
82
74
10
6
5
5
5
4
4
1
1
Host
Mouse
107
25
18
Clonality
Monoclonal
Conjugate
This NFAT1 antibody is un-conjugated
6
6
6
4
4
4
3
3
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
Application
Chromatin Immunoprecipitation (ChIP), ELISA, Gel Shift (GS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
88
52
39
33
21
19
19
17
15
9
3
2
2
1
1
1
Supplier
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Catalog No. ABIN152663
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN453985 EIA IHC (p) WB Goat Internal Region Log in to see Polyclonal 1
1 ABIN959000 IHC (p) WB Rabbit C-Term Log in to see Polyclonal
1 ABIN2626029 IHC (p) WB Rabbit IgG AA 895-913 Log in to see Polyclonal
1 ABIN705830 IHC (p) WB HRP Rabbit IgG Log in to see Polyclonal
1 ABIN710720 IHC (p) WB HRP Rabbit IgG pSer330 Log in to see Polyclonal
1 ABIN748862 IHC (p) WB HRP Rabbit IgG pSer326 Log in to see Polyclonal
1 ABIN4339303 ELISA ICC IF IHC (fro) IHC (p) WB DyLight 488 Mouse IgG1 Log in to see 25A10-D6-D2
1 ABIN4339308 ELISA ICC IF IHC (fro) IHC (p) WB Alexa Fluor 647 Mouse IgG1 Log in to see 25A10-D6-D2
1 ABIN4339309 ELISA ICC IF IHC (fro) IHC (p) WB Alexa Fluor 700 Mouse IgG1 Log in to see 25A10-D6-D2
1 ABIN4339306 ELISA ICC IF IHC (fro) IHC (p) WB Alexa Fluor 405 Mouse IgG1 Log in to see 25A10-D6-D2
1 ABIN4339307 ELISA ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Mouse IgG1 Log in to see 25A10-D6-D2
1 ABIN4339298 ELISA ICC IF IHC (fro) IHC (p) WB DyLight 405 Mouse IgG1 Log in to see 25A10-D6-D2
1 ABIN4339305 ELISA ICC IF IHC (fro) IHC (p) WB DyLight 680 Mouse IgG1 Log in to see 25A10-D6-D2
1 ABIN4339297 ELISA ICC IF IHC (fro) IHC (p) WB DyLight 350 Mouse IgG1 Log in to see 25A10-D6-D2
1 ABIN4339300 GS ICC IF IHC IHC (p) IP WB PerCP Mouse IgG1 Log in to see 25A10-D6-D2
1 ABIN4339302 ELISA ICC IF IHC (fro) IHC (p) WB DyLight 755 Mouse IgG1 Log in to see 25A10-D6-D2
1 ABIN705823 IHC (p) WB Biotin Rabbit IgG Log in to see Polyclonal
1 ABIN710713 IHC (p) WB Biotin Rabbit IgG pSer330 Log in to see Polyclonal
1 ABIN748855 IHC (p) WB Biotin Rabbit IgG pSer326 Log in to see Polyclonal
1 ABIN4339301 GS ICC IF IHC IHC (p) IP WB Allophycocyanin Mouse IgG1 AA 51-69 Log in to see 25A10-D6-D2

Top referenced anti-NFAT1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-NFAT1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Chromatin Immunoprecipitation (ChIP) 1 Antibodies 1 Antibodies
ELISA 15 Antibodies 16 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Gel Shift (GS) 4 Antibodies 4 Antibodies
Immunocytochemistry (ICC) 13 Antibodies 13 Antibodies
Immunofluorescence (IF) 13 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 39 Antibodies
Immunohistochemistry (IHC) 5 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 10 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 27 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Western Blotting (WB) 38 Antibodies
Immunochromatography (IC) 1 Antibodies

General

Antigen Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(146), (82), (74), (10), (6), (5), (5), (5), (4), (4), (1), (1)
Host Mouse
(107), (25), (18)
Clonality (Clone)
Monoclonal   ( )
Conjugate This NFAT1 antibody is un-conjugated
(6), (6), (6), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Chromatin Immunoprecipitation (ChIP), ELISA, Gel Shift (GS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(88), (52), (39), (33), (21), (19), (19), (17), (15), (9), (3), (2), (2), (1), (1), (1)
Pubmed 8 references available
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Product Details anti-NFAT1 Antibody

Target Details NFAT1 Application Details Handling References for anti-NFAT1 Antibody (ABIN152663) Images
Specificity Detects nuclear factor of activated T-cells (NFAT). This does not cross react with NFAT2 (NFATc, NFATc1).
Purification Protein G purified
Immunogen Synthetic peptide corresponding to residues A(51) I S S P S G L A Y P D D V L D Y G L(69) of mouse NFATc2-A, B and C isoforms.
Clone 25A10-D6-D2
Isotype IgG1

Target Details NFAT1

Product Details anti-NFAT1 Antibody Application Details Handling References for anti-NFAT1 Antibody (ABIN152663) Images back to top
Antigen
Alternative Name NFATC2/NFAT1 (NFAT1 Antibody Abstract)
Background Gene Symbol: NFATC2
Molecular Weight Theoretical MW: 140 kDa
Gene ID 4773
Pathways RTK Signaling, WNT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-NFAT1 Antibody Target Details NFAT1 Handling References for anti-NFAT1 Antibody (ABIN152663) Images back to top
Application Notes Western Blot 1 μg/mL, Chromatin Immunoprecipitation 1 - 3 μL, Immunocytochemistry/Immunofluorescence 1:100 - 1:1000, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 1:50 - 1:2000, Immunohistochemistry-Frozen 1:50 - 1:2000, Gel Super Shift Assays 1:1 - 1:100, Sandwich ELISA 1:100 - 1:2000ChIP usage was reported in scientific literature (PMID: 15347678). In WB: Detects an approx. 140 kDa protein representing phosphorylated NFAT1 in restiing immune cell and approx. 120 kDa protein in stimulated cells that represents a fully dephosphorylated NAFT1. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-NFAT1 Antibody Target Details NFAT1 Application Details References for anti-NFAT1 Antibody (ABIN152663) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.

References for anti-NFAT1 Antibody (ABIN152663)

Product Details anti-NFAT1 Antibody Target Details NFAT1 Application Details Handling Images back to top
Product cited in:

Liu, Han, Han, He: "Mesenchymal stem cell-conditioned media suppresses inflammation-associated overproliferation of pulmonary artery smooth muscle cells in a rat model of pulmonary hypertension." in: Experimental and therapeutic medicine, Vol. 11, Issue 2, pp. 467-475, 2016

Cotroneo, Ashek, Wang, Wharton, Dubois, Bozorgi, Busbridge, Alavian, Wilkins, Zhao: "Iron homeostasis and pulmonary hypertension: iron deficiency leads to pulmonary vascular remodeling in the rat." in: Circulation research, Vol. 116, Issue 10, pp. 1680-90, 2015 (Sample species: Rat (Rattus)). Further details: Western Blotting,Immunohistochemistry

Iampietro, Morissette, Gravel, Flamand: "Inhibition of interleukin-2 gene expression by human herpesvirus 6B U54 tegument protein." in: Journal of virology, Vol. 88, Issue 21, pp. 12452-63, 2014 (Sample species: Human). Further details: Western Blotting

Alpini, Franchitto, Demorrow, Onori, Gaudio, Wise, Francis, Venter, Kopriva, Mancinelli, Carpino, Stagnitti, Ueno, Han, Meng, Glaser: "Activation of alpha(1) -adrenergic receptors stimulate the growth of small mouse cholangiocytes via calcium-dependent activation of nuclear factor of activated T cells 2 and specificity protein 1." in: Hepatology (Baltimore, Md.), Vol. 53, Issue 2, pp. 628-39, 2011 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry

Landry, Banerjee, Taylor, Aplan, Singer, Wu: "Chromatin remodeling complex NURF regulates thymocyte maturation." in: Genes & development, Vol. 25, Issue 3, pp. 275-86, 2011

Bonnet, Rochefort, Sutendra, Archer, Haromy, Webster, Hashimoto, Bonnet, Michelakis: "The nuclear factor of activated T cells in pulmonary arterial hypertension can be therapeutically targeted." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 27, pp. 11418-23, 2007

Barlic, McDermott, Merrell, Gonzales, Via, Murphy: "Interleukin (IL)-15 and IL-2 reciprocally regulate expression of the chemokine receptor CX3CR1 through selective NFAT1- and NFAT2-dependent mechanisms." in: The Journal of biological chemistry, Vol. 279, Issue 47, pp. 48520-34, 2004

Kornau, Schenker, Kennedy, Seeburg: "Domain interaction between NMDA receptor subunits and the postsynaptic density protein PSD-95." in: Science (New York, N.Y.), Vol. 269, Issue 5231, pp. 1737-40, 1995

Images

Product Details anti-NFAT1 Antibody Target Details NFAT1 Application Details Handling References for anti-NFAT1 Antibody (ABIN152663) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-NFAT1 antibody (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2) (ABIN152663) Immunohistochemistry-Paraffin: NFATC2/NFAT1 Antibody (25A10.D6.D2) [ABIN152663] - Can...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-NFAT1 antibody (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2) (ABIN152663) Immunohistochemistry-Paraffin: NFATC2/NFAT1 Antibody (25A10.D6.D2) [ABIN152663] - Bot...
Immunoprecipitation (IP) image for anti-NFAT1 antibody (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2) (ABIN152663) Chromatin Immunoprecipitation: NFATC2/NFAT1 Antibody (25A10.D6.D2) [ABIN152663] - Ana...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-NFAT1 antibody (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2) (ABIN152663) Immunohistochemistry-Paraffin: NFATC2/NFAT1 Antibody (25A10.D6.D2) [ABIN152663] - Bot...
Immunofluorescence (IF) image for anti-NFAT1 antibody (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2) (ABIN152663) Immunocytochemistry/Immunofluorescence: NFATC2/NFAT1 Antibody (25A10.D6.D2) [ABIN1526...
Immunofluorescence (IF) image for anti-NFAT1 antibody (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2) (ABIN152663) Immunocytochemistry/Immunofluorescence: NFATC2/NFAT1 Antibody (25A10.D6.D2) [ABIN1526...
Immunofluorescence (IF) image for anti-NFAT1 antibody (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2) (ABIN152663) Immunocytochemistry/Immunofluorescence: NFATC2/NFAT1 Antibody (25A10.D6.D2) [ABIN1526...
Immunofluorescence (IF) image for anti-NFAT1 antibody (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2) (ABIN152663) Immunocytochemistry/Immunofluorescence: NFATC2/NFAT1 Antibody (25A10.D6.D2) [ABIN1526...