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anti-Rat (Rattus) NFAT1 antibody for Enzyme Immunoassay

Recommended NFAT1 Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1) Antibodies
  • AI607462
  • NF-ATc2
  • NFAT1
  • NFAT1-D
  • NFATC2
  • NFATP
  • Nfatp
Epitope
Internal Region
33
15
14
13
7
7
6
4
4
3
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
141
70
63
6
2
2
1
1
1
Host
Goat
101
27
17
Clonality
Polyclonal
Conjugate
This NFAT1 antibody is un-conjugated
6
6
6
4
4
4
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
88
47
36
33
30
24
21
19
18
14
2
2
2
1
1
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Catalog No. ABIN453985
$ 379.50
Plus shipping costs $45.00

Top referenced anti-NFAT1 antibody for Enzyme Immunoassay

Similar anti-NFAT1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Dog (Canine) Cow (Bovine)
Western Blotting (WB) 85 Antibodies 39 Antibodies 33 Antibodies 7 Antibodies 3 Antibodies
Immunoprecipitation (IP) 23 Antibodies 16 Antibodies 14 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 37 Antibodies 25 Antibodies 26 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 30 Antibodies 17 Antibodies 17 Antibodies 2 Antibodies 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies

General

Antigen Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1) Antibodies
Epitope Internal Region
(33), (15), (14), (13), (7), (7), (6), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(141), (70), (63), (6), (2), (2), (1), (1), (1)
Host Goat
(101), (27), (17)
Clonality
Polyclonal
Conjugate This NFAT1 antibody is un-conjugated
(6), (6), (6), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(88), (47), (36), (33), (30), (24), (21), (19), (18), (14), (2), (2), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-NFAT1 Antibody

Target Details NFAT1 Application Details Handling References for anti-NFAT1 Antibody (ABIN453985) Images
Sequence c-QQFRTGLSSP DARYQ
Specificity This antibody is expected to recognize all three reported isoforms of NFATC2 (NP_036472.2, NP_775114.1, NP_001129493.1).
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Rat, Canine, Bovine.
Species reactivity (tested):Human.
Purification Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
Immunogen Peptide with sequence from the internal region of the protein sequence according to NP_036472.2, NP_775114.1, NP_001129493.1. Genename: NFATC2

Target Details NFAT1

Product Details anti-NFAT1 Antibody Application Details Handling References for anti-NFAT1 Antibody (ABIN453985) Images back to top
Antigen
Alternative Name NFATC2 (NFAT1 Antibody Abstract)
Background Nuclear factor of activated T cells (NFAT) is a family of transcription factors implicated in multiple biological processes including cytokine gene expression, cardiac hypertrophy and adipocyte differentiation. NFAT1 (also known as NFATc2 or NFATp) is a member of this family that is regulated by the calcium-dependent phosphatase calcineurin. When calcineurin is activated by calcium it dephosphorylates multiple residues in the regulatory domain of NFAT1, leading to its translocation to the nucleus and activation of its transcriptional activity. Once in the nucleus, NFAT proteins act synergistically with the AP-1 transcription factor complex to regulate the expression of multiple genes. Serine 54 in mouse NFAT1 has been shown to be important in the regulation of its transcriptional activity.Synonyms: NFAT pre-existing subunit, NFAT-1, NFATC2, NFATP, Nuclear factor of activated T-cells, T-cell transcription factor NFAT1, cytoplasmic 2
Gene ID 4773
NCBI Accession NP_036472
UniProt Q13469
Pathways RTK Signaling, WNT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-NFAT1 Antibody Target Details NFAT1 Handling References for anti-NFAT1 Antibody (ABIN453985) Images back to top
Application Notes Peptide ELISA: 1/64000. Western blot: 0.3 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-NFAT1 Antibody Target Details NFAT1 Application Details References for anti-NFAT1 Antibody (ABIN453985) Images back to top
Concentration 0.5 mg/mL
Buffer Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-NFAT1 Antibody (ABIN453985)

Product Details anti-NFAT1 Antibody Target Details NFAT1 Application Details Handling Images back to top
Background publications

Celil Aydemir, Lee, Won Kim, Gardner, Prince, Mok Ahn, Lee, Young-In Lee: "Nuclear factor of activated T cell mediates proinflammatory gene expression in response to mechanotransduction." in: Annals of the New York Academy of Sciences, Vol. 1117, pp. 138-42, 2007

Images

Product Details anti-NFAT1 Antibody Target Details NFAT1 Application Details Handling References for anti-NFAT1 Antibody (ABIN453985) back to top
Supplier Images
 image for anti-NFAT1 antibody (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2) (Internal Region) (ABIN453985) anti-Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1) (Internal Region) antibody
 image for anti-NFAT1 antibody (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2) (Internal Region) (ABIN453985) anti-Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1) (Internal Region) antibody (Image 2)