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anti-Mouse (Murine) NFATC1 antibody for Immunoprecipitation

Recommended NFATC1 Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1) Antibodies
  • 2210017P03Rik
  • AI449492
  • AV076380
  • NF-ATc
  • nf-atc
  • NF-ATC
  • NF-ATc1
  • NFAT
  • NFAT2
  • nfat2
  • nfatc
  • NFATc
  • NFATC1
  • Nfatcb
  • NFATmac
  • si:ch211-198c22.2
Reactivity
Human, Mouse (Murine)
147
36
31
2
1
1
1
1
1
1
Host
Mouse
100
46
1
Clonality
Monoclonal
Conjugate
This NFATC1 antibody is un-conjugated
5
4
4
4
4
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
118
52
41
14
11
8
8
8
7
3
3
3
2
2
2
1
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Catalog No. ABIN967559
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089556724 ABIN152773 ChIP GS ICC IF IHC (p) IP IHC WB Mouse IgG1 AA 1-654 Log in to see 7A6 1

Top referenced anti-NFATC1 antibody for Immunoprecipitation

Similar anti-NFATC1 Antibodies

Application / Reactivity Mouse (Murine) Human
Chromatin Immunoprecipitation (ChIP) 1 Antibodies 3 Antibodies
ELISA 5 Antibodies 53 Antibodies
Flow Cytometry (FACS) 6 Antibodies
Gel Shift (GS) 1 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 11 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 4 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Mass Cytometry (CyTOF) 2 Antibodies
Western Blotting (WB) 18 Antibodies

General

Antigen Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1) Antibodies
Reactivity Human, Mouse (Murine)
(147), (36), (31), (2), (1), (1), (1), (1), (1), (1)
Host Mouse
(100), (46), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This NFATC1 antibody is un-conjugated
(5), (4), (4), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(118), (52), (41), (14), (11), (8), (8), (8), (7), (3), (3), (3), (2), (2), (2), (1)
Pubmed 5 references available
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Product Details anti-NFATC1 Antibody

Target Details NFATC1 Application Details Handling References for anti-NFATC1 Antibody (ABIN967559) Images
Brand BD Pharmingen™
Cross-Reactivity Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Clone 7A6
Isotype IgG1, kappa

Target Details NFATC1

Product Details anti-NFATC1 Antibody Application Details Handling References for anti-NFATC1 Antibody (ABIN967559) Images back to top
Antigen
Alternative Name NF-ATc1 (NFATC1 Antibody Abstract)
Background The NF-AT family of transcription factors are regulators of early immune response genes in T cells following their activation by CD40L, FasL and nterleukins including IL-2, -3, -4 and -5. NF-AT proteins function in both signal transduction and transcription control. The DNA binding, active NF-AT complex contains a cytoplasmic, Ca2+/calcineurin dependent, cyclosporin sensitive subunit, designated NF-ATc, which is present in the cytoplasm of resting cells. Upon cell activation, NF-ATc is dephosphorylated and thus activated by calcineurin, resulting in rapid translocation of NF-ATc to the nucleus. The active NF-AT complex also contains a nuclear component, NF-ATn. In vitro, NF-AT cooperates with the mitogenic transcription factor AP-1 to induce multiple cytokine genes. NF-ATc is encoded by four genes: NF-ATc1 (originally named NF-ATc), NF-ATc2 (originally named NF-ATp), NF-ATc3 (NF-AT3) and NF- ATc4 (NF-AT4). These proteins are differentially expressed in tissues, suggesting that each may activate distinct sets of genes. NF-ATc1 is expressed in lymphoid organs and is upregulated in activated T cell and thymocytes. NF-ATc1 is required for the development of T cells and has been shown to contribute to the production of IL-2 and the development of Th2 responses. 7A6 recognizes human and mouse NF-ATc1. The antibody recognizes a region between amino acids 197 to 304 of human NF-ATc1, which lies outside the region of homology between NF-ATc1 and NF-ATc2.3 Please note that in the original publication for the antibody (Northrop et al. 1994), the clone name 7A6 is not specifically identified.
Molecular Weight 100-120 kDa
Pathways RTK Signaling, WNT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-NFATC1 Antibody Target Details NFATC1 Handling References for anti-NFATC1 Antibody (ABIN967559) Images back to top
Application Notes 7A6 may be used for western blot analysis of NF-ATc1 (1-2 µg/ml). Note that while the predicted M W of NF-ATc is ~100 kDa, the observed M W may range between 100 to 120 kDa due to post-translational modification of NF-ATc. Other applications include immunoprecipitation (2 µg/ml), mobility shift assays (EMSA) and immunofluorescence microscopy of paraformaldehyde-fixed, tissue cultured cells. Jurkat human T cells (ATCC TIB-152) are suggested as a positive control for expression of NF-ATc.
Comment

Related Products: ABIN967389, ABIN968537

Restrictions For Research Use only

Handling

Product Details anti-NFATC1 Antibody Target Details NFATC1 Application Details References for anti-NFATC1 Antibody (ABIN967559) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-NFATC1 Antibody (ABIN967559)

Product Details anti-NFATC1 Antibody Target Details NFATC1 Application Details Handling Images back to top
Product cited in:

Yoshida, Nishina, Takimoto, Marengère, Wakeham, Bouchard, Kong, Ohteki, Shahinian, Bachmann, Ohashi, Penninger, Crabtree, Mak: "The transcription factor NF-ATc1 regulates lymphocyte proliferation and Th2 cytokine production." in: Immunity, Vol. 8, Issue 1, pp. 115-24, 1998

Timmerman, Healy, Ho, Chen, Goodnow, Crabtree: "Redundant expression but selective utilization of nuclear factor of activated T cells family members." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 6, pp. 2735-40, 1997

Timmerman, Clipstone, Ho, Northrop, Crabtree: "Rapid shuttling of NF-AT in discrimination of Ca2+ signals and immunosuppression." in: Nature, Vol. 383, Issue 6603, pp. 837-40, 1996

Northrop, Ho, Chen, Thomas, Timmerman, Nolan, Admon, Crabtree: "NF-AT components define a family of transcription factors targeted in T-cell activation." in: Nature, Vol. 369, Issue 6480, pp. 497-502, 1994

Crabtree: "Contingent genetic regulatory events in T lymphocyte activation." in: Science (New York, N.Y.), Vol. 243, Issue 4889, pp. 355-61, 1989

Images

Product Details anti-NFATC1 Antibody Target Details NFATC1 Application Details Handling References for anti-NFATC1 Antibody (ABIN967559) back to top
Supplier Images
Western Blotting (WB) image for anti-NFATC1 antibody (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1) (ABIN967559) Western blot analysis of NFATc1. Lysate from Jurkat T cells was probed with anti-NF-A...
 image for anti-NFATC1 antibody (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1) (ABIN967559) anti-Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1) antibody (Image 2)
Western Blotting (WB) image for anti-NFATC1 antibody (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1) (ABIN967559) anti-Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1) antibody (Image 3)