Mouse (Murine) SHC1 ELISA Kit

Recommended SHC1 Product (supplied by: Log in to see )

Antigen
SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1) ELISA Kits
  • SHC1
  • SHC
  • SHCA
  • P66shc
  • si:dkey-246i14.4
  • wu:fc10h03
  • shc
  • p66shc
  • Shc
  • ShcA
  • p66
  • SHC (Src homology 2 domain containing) transforming protein 1
  • Shc1p
  • squalene--hopene cyclase
  • src homology 2 domain-containing transforming protein C1
  • SHC1
  • shc1
  • LOC100229782
  • Shc1
Epitope
pTyr427, total
Reactivity
Human, Mouse (Murine), Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
12
8
7
Method Type
Sandwich ELISA
Application
ELISA
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Catalog No. ABIN4889789
$ 628.10
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
1 ABIN4889788 Sandwich ELISA Cell and Tissue Culture Lysate Log in to see
1 ABIN1380079 Cell ELISA Log in to see
1 ABIN1380080 Cell ELISA Log in to see
1 ABIN5526713 Cell ELISA Adherent Cell Culture Log in to see
1 ABIN2114917 Cell ELISA Log in to see
1 ABIN2114918 Cell ELISA Log in to see
1 ABIN2685476 Cell ELISA Cell Samples, Suspension Cell Culture Log in to see

Similar SHC1 ELISA Kits

Application / Reactivity Human Rat (Rattus)
ELISA 13 ELISA Kits 9 ELISA Kits

General

Antigen SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1) ELISA Kits
Epitope pTyr427, total
Reactivity Human, Mouse (Murine), Rat (Rattus)
Kits with alternative reactivity to:
(12), (8), (7)
Method Type Sandwich ELISA
Application ELISA
Supplier Log in to see

Product Details SHC1 ELISA Kit

Target details Application Details Handling Images
Purpose Human, Mouse and Rat Phospho-SHC (Tyr427) and Total SHC ELISA Kit. This assay semi-quantitatively measures SHC phosphorylated at Tyrosine-427 as well as total SHC in cell lysate samples.
Sample Type Cell and Tissue Culture Lysate
Detection Method Colorimetric
Analytical Method Semi-Quantitative
Specificity This ELISA kit recognizes Human, Mouse and Rat SHC phosphorylated at site Tyrosine-427 as well as total SHC.
Characteristics
  • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
  • Screen numerous different cell lysates without performing a Western Blot analysis
  • Minimal hands-on time, convenient, and non-radioactive material
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Anti-Phospho Antibody
  • Anti-Pan Antibody
  • HRP-Conjugated Secondary Antibody
  • Streptavidin-Conjugated HRP
  • Assay Diluent
  • TMB One-Step Substrate
  • Stop Solution
  • Lysis Buffer
  • Positive Control Sample
Material not included
  • Distilled or deionized water
  • 100 mL and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 μL to 1 mL volumes
  • Adjustable 1-25 mL pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm

Target details

Product Details SHC1 ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name SHC (SHC1 ELISA Kit Abstract)
Gene ID 6464
UniProt P29353, P98083, O70143
Research Area Signaling, Alzheimer's Disease, Tyrosine Kinases
Pathways RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, ER-Nucleus Signaling, Signaling Events mediated by VEGFR1 and VEGFR2

Application Details

Product Details SHC1 ELISA Kit Target details Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Sample Volume 100 μL
Plate Pre-coated
Protocol
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 μL of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Restrictions For Research Use only

Handling

Product Details SHC1 ELISA Kit Target details Application Details Images back to top
Storage -20 °C
Storage Comment Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
Expiry Date 6 months