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Human TRKA ELISA Kit

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Antigen
Neurotrophic Tyrosine Kinase, Receptor, Type 1 (NTRK1) ELISA Kits
  • C80751
  • CAPM1
  • CFTD
  • CTRKA
  • hscp30
  • MTC
  • NEM1
  • OK/SW-cl.5
  • p140-TrkA
  • Tkr
  • TM-5
  • TM3
  • TM5
  • TM30
  • TM30nm
  • TPMsk3
  • trk
  • TRK
  • Trk
  • Trk-A
  • TRK1
  • TRKA
  • TrkA
  • trkA
  • trka
Epitope
AA 33-407
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
19
16
9
1
Methode Type
Sandwich ELISA
Detection Range
156-10000 pg/mL
Minimum Detection Limit
156 pg/mL
Application
ELISA
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Catalog No. ABIN1672815
$ 420.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.306289 ABIN2708402 Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Tissue Homogenate 156-10000 pg/mL 156 pg/mL Log in to see
0.306289 ABIN2114671 Cell Culture Supernatant, Cell Lysate, Tissue Homogenate Log in to see
0.0012155632 ABIN819165 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate 23.44-1500 pg/mL 23.44 pg/mL Log in to see
0.0012155632 ABIN3199233 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 468.75-30000 pg/mL 468.75 pg/mL Log in to see
0.0012155632 ABIN1380759 Cell ELISA Log in to see
0.0012155632 ABIN1380760 Cell ELISA Log in to see
0.0012155632 ABIN1381567 Cell ELISA Log in to see
0.0012155632 ABIN2042710 Sandwich ELISA 6-10000 pg/mL 6 pg/mL Log in to see
0.0012155632 ABIN2685114 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Plasma, Serum, Tissue Lysate 156-10000 pg/mL 156 pg/mL Log in to see
0.0012155632 ABIN2114632 Cell ELISA Log in to see
0.0012155632 ABIN2114633 Cell ELISA Log in to see
0.0012155632 ABIN1136648 78-5000 pg/mL 78 pg/mL Log in to see
0.0012155632 ABIN2114072 Cell ELISA Log in to see
0.0012155632 ABIN2685115 Cell ELISA Cell Samples, Suspension Cell Culture Log in to see
0.0012155632 ABIN2116382 156-10000 pg/mL 156 pg/mL Log in to see
0.0012155632 ABIN2948295 78-5000 pg/mL 78 pg/mL Log in to see

Top referenced TRKA ELISA Kit for ELISA

Similar TRKA ELISA Kits

Application / Reactivity Human
ELISA 17 ELISA Kits

General

Antigen Neurotrophic Tyrosine Kinase, Receptor, Type 1 (NTRK1) ELISA Kits
Epitope AA 33-407
Reactivity Human
Kits with alternative reactivity to:
(19), (16), (9), (1)
Methode Type Sandwich ELISA
Detection Range 156-10000 pg/mL
Minimum Detection Limit 156 pg/mL
Application ELISA
Pubmed 2 references available
Supplier Log in to see

Product Details TRKA ELISA Kit

Target details Application Details Handling References for TRKA Kit (ABIN1672815) Images
Purpose For quantitative detection of human TrkA in cell culture supernates, cell lysates and tissue homogenates.
Brand PicoKine™
Sample Type Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Detection Method Colorimetric
Specificity Natural and recombinant human TrkA
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- human TrkA antibod - 1
  • Lyophilized recombinant human TrkA standar - 10ng/tubex2
  • Biotinylated anti- human TrkA antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: A33-E407

Target details

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Antigen
Alternative Name NTRK1 (NTRK1 ELISA Kit Abstract)
Background Tropomyosin receptor kinase A(TrkA) are efficacious in attenuating skeletal pain.1 TrkA mutants were able to activate signaling cascades and were even more efficient in promoting neurite outgrowth than the wild-type receptor.2 TrkA is part of a sub-family of protein kinases which includes TrkB and TrkC. Also, there are other neurotrophic factors structurally related to NGF: BDNF(for Brain-Derived Neurotrophic Factor), NT-3(for Neurotrophin-3) and NT-4(for Neurotrophin-4). While TrkA mediates the effects of NGF, TrkB is bound and activated by BDNF, NT-4, and NT-3. Further, TrkC binds and is activated by NT-3.3 TrkA receptor was found in kehumanoconus-affected corneas, along with an increased level of repressor isoform of Sp3 transcription factor.4
Gene ID 4914
UniProt P04629
Pathways RTK Signaling, Neurotrophin Signaling Pathway

Application Details

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Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human TrkA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TrkA has been precoated onto 96-well plates. Standards (NSO,A33-E407) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TrkA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TrkA amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human TrkA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates or tissue lysates to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TrkA standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 928, Standard deviation: 41.76, CV(%): 4.5
  • Sample 2: n=16, Mean(pg/ml): 3426, Standard deviation: 212.4, CV(%): 6.2
  • Sample 3: n=16, Mean(pg/ml): 6042, Standard deviation: 308.1, CV(%): 5.1,
  • Sample 1: n=24, Mean(pg/ml): 1044, Standard deviation: 63.7, CV(%): 6.1
  • Sample 2: n=24, Mean(pg/ml): 3020, Standard deviation: 223.5, CV(%): 7.4
  • Sample 3: n=24, Mean(pg/ml): 6625, Standard deviation: 450.5, CV(%): 6.8
Restrictions For Research Use only

Handling

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Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for TRKA Kit (ABIN1672815)

Product Details TRKA ELISA Kit Target details Application Details Handling Images back to top
Background publications

Georgieva, de Pablo, Sanchis, Comella, Llovera: "Ubiquitination of TrkA by Nedd4-2 regulates receptor lysosomal targeting and mediates receptor signaling." in: Journal of neurochemistry, Vol. 117, Issue 3, pp. 479-93, 2011

Benito-Gutiérrez, Garcia-Fernàndez, Comella: "Origin and evolution of the Trk family of neurotrophic receptors." in: Molecular and cellular neurosciences, Vol. 31, Issue 2, pp. 179-92, 2006

Images

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Supplier Images
ELISA image for TRKA ELISA Kit (Neurotrophic Tyrosine Kinase, Receptor, Type 1) (ABIN1672815) Human TrkA PicoKine ELISA Kit standard curve