You are viewing an incomplete version of our website. Please click to reload the website as full version.

Human TRKA ELISA Kit

Recommended TRKA Product (supplied by: Log in to see )

Antigen
Neurotrophic Tyrosine Kinase, Receptor, Type 1 (NTRK1) ELISA Kits
  • trk
  • trka
  • TRKA
  • MTC
  • TRK
  • TRK1
  • Trk-A
  • p140-TrkA
  • C80751
  • Tkr
  • TrkA
  • Trk
  • CTRKA
  • trkA
  • CAPM1
  • CFTD
  • NEM1
  • OK/SW-cl.5
  • TM-5
  • TM3
  • TM30
  • TM30nm
  • TM5
  • TPMsk3
  • hscp30
  • neurotrophic tyrosine kinase, receptor, type 1
  • tropomyosin 3
  • ntrk1
  • NTRK1
  • LOC100354292
  • Ntrk1
  • TPM3
Epitope
AA 33-407
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
19
18
11
1
Method Type
Sandwich ELISA
Detection Range
156-10000 pg/mL
Minimum Detection Limit
156 pg/mL
Application
ELISA
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN1672815
$ 420.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
10.893343 ABIN3199233 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 468.75-30000 pg/mL 468.75 pg/mL Log in to see
10.893343 ABIN4884901 Sandwich ELISA Plasma, Cell Culture Supernatant, Serum 2-500 ng/mL 2 ng/mL Log in to see
10.893343 ABIN2708402 Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Tissue Homogenate 156-10000 pg/mL 156 pg/mL Log in to see
10.893343 ABIN2114671 Cell Culture Supernatant, Cell Lysate, Tissue Homogenate Log in to see
1 ABIN819165 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate 23.5-1500 pg/mL 23.5 pg/mL Log in to see
1 ABIN1380759 Cell ELISA Log in to see
1 ABIN1380760 Cell ELISA Log in to see
1 ABIN1381567 Cell ELISA Log in to see
1 ABIN5521293 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 78-5000 pg/mL 78 pg/mL Log in to see
1 ABIN2042710 Sandwich ELISA 6-10000 pg/mL 6 pg/mL Log in to see
1 ABIN2685114 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Plasma, Serum, Tissue Lysate 156-10000 pg/mL 156 pg/mL Log in to see
1 ABIN2114633 Cell ELISA Log in to see
1 ABIN2114632 Cell ELISA Log in to see
1 ABIN2114072 Cell ELISA Log in to see
1 ABIN1136648 78-5000 pg/mL 78 pg/mL Log in to see
1 ABIN2685115 Cell ELISA Cell Samples, Suspension Cell Culture Log in to see
1 ABIN2116382 156-10000 pg/mL 156 pg/mL Log in to see
1 ABIN2948295 78-5000 pg/mL 78 pg/mL Log in to see

Similar TRKA ELISA Kits

Application / Reactivity Human
ELISA 19 ELISA Kits

General

Antigen Neurotrophic Tyrosine Kinase, Receptor, Type 1 (NTRK1) ELISA Kits
Epitope AA 33-407
Reactivity Human
Kits with alternative reactivity to:
(19), (18), (11), (1)
Method Type Sandwich ELISA
Detection Range 156-10000 pg/mL
Minimum Detection Limit 156 pg/mL
Application ELISA
Pubmed 2 references available
Supplier Log in to see

Product Details TRKA ELISA Kit

Target details Application Details Handling References for TRKA Kit (ABIN1672815) Images
Purpose Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human trkA
Brand PicoKine™
Sample Type Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Analytical Method Quantitative
Specificity Expression system for standard: NSO,A33-E407
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: A33-E407

Target details

Product Details TRKA ELISA Kit Application Details Handling References for TRKA Kit (ABIN1672815) Images back to top
Antigen
Alternative Name NTRK1 (NTRK1 ELISA Kit Abstract)
Background

Protein Function: Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF which is its primary ligand, it can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival. Upon dimeric NGF ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation. Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival. Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival. Through SHC1 and SH2B1 controls a Ras- PI3 kinase-AKT1 signaling cascade that is also regulating survival. In absence of ligand and activation, may promote cell death, making the survival of neurons dependent on trophic factors.

Background: Tropomyosin receptor kinase A(TrkA) are efficacious in attenuating skeletal pain.1 TrkA mutants were able to activate signaling cascades and were even more efficient in promoting neurite outgrowth than the wild-type receptor.2 TrkA is part of a sub-family of protein kinases which includes TrkB and TrkC. Also, there are other neurotrophic factors structurally related to NGF: BDNF(for Brain-Derived Neurotrophic Factor), NT-3(for Neurotrophin-3) and NT-4(for Neurotrophin-4). While TrkA mediates the effects of NGF, TrkB is bound and activated by BDNF, NT-4, and NT-3. Further, TrkC binds and is activated by NT-3.3 TrkA receptor was found in kehumanoconus-affected corneas, along with an increased level of repressor isoform of Sp3 transcription factor.4

Synonyms: High affinity nerve growth factor receptor,2.7.10.1,Neurotrophic tyrosine kinase receptor type 1,TRK1-transforming tyrosine kinase protein,Tropomyosin-related kinase A,Tyrosine kinase receptor,Tyrosine kinase receptor A,Trk-A,gp140trk,p140-TrkA,NTRK1,MTC, TRK, TRKA,

Full Gene Name: High affinity nerve growth factor receptor

Cellular Localisation: Cell membrane, Single-pass type I membrane protein . Early endosome membrane, Single-pass type I membrane protein . Late endosome membrane, Single-pass type I membrane protein . Internalized to endosomes upon binding of NGF or NTF3 and further transported to the cell body via a retrograde axonal transport. Localized at cell membrane and early endosomes before nerve growth factor (NGF) stimulation. Recruited to late endosomes after NGF stimulation. Colocalized with RAPGEF2 at late endosomes (By similarity)..
Gene ID 4914
UniProt P04629
Pathways RTK Signaling, Neurotrophin Signaling Pathway, cAMP Metabolic Process

Application Details

Product Details TRKA ELISA Kit Target details Handling References for TRKA Kit (ABIN1672815) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Comment

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.

Tissue Specificity: Isoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by pluripotent neural stem and neural crest progenitors. .

Plate Pre-coated,Strips (12 x 8)
Protocol human TrkA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TrkA has been precoated onto 96-well plates. Standards (NSO,A33-E407) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TrkA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TrkA amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human TrkA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates or tissue lysates to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TrkA standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 928, Standard deviation: 41.76, CV(%): 4.5
  • Sample 2: n=16, Mean(pg/ml): 3426, Standard deviation: 212.4, CV(%): 6.2
  • Sample 3: n=16, Mean(pg/ml): 6042, Standard deviation: 308.1, CV(%): 5.1,
  • Sample 1: n=24, Mean(pg/ml): 1044, Standard deviation: 63.7, CV(%): 6.1
  • Sample 2: n=24, Mean(pg/ml): 3020, Standard deviation: 223.5, CV(%): 7.4
  • Sample 3: n=24, Mean(pg/ml): 6625, Standard deviation: 450.5, CV(%): 6.8
Restrictions For Research Use only

Handling

Product Details TRKA ELISA Kit Target details Application Details References for TRKA Kit (ABIN1672815) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for TRKA Kit (ABIN1672815)

Product Details TRKA ELISA Kit Target details Application Details Handling Images back to top
Background publications

Georgieva, de Pablo, Sanchis, Comella, Llovera: "Ubiquitination of TrkA by Nedd4-2 regulates receptor lysosomal targeting and mediates receptor signaling." in: Journal of neurochemistry, Vol. 117, Issue 3, pp. 479-93, 2011

Benito-Gutiérrez, Garcia-Fernàndez, Comella: "Origin and evolution of the Trk family of neurotrophic receptors." in: Molecular and cellular neurosciences, Vol. 31, Issue 2, pp. 179-92, 2006

Images

Product Details TRKA ELISA Kit Target details Application Details Handling References for TRKA Kit (ABIN1672815) back to top
Supplier Images
ELISA image for TRKA ELISA Kit (Neurotrophic Tyrosine Kinase, Receptor, Type 1) (ABIN1672815) Human TrkA PicoKine ELISA Kit standard curve