Mouse (Murine) TRKB ELISA Kit

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Antigen
Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) ELISA Kits
  • trkb
  • trkb-b
  • xtrkb
  • si:ch211-220b11.1
  • trkB2
  • NTRK2
  • ntrk2
  • gp145-trkb
  • TRKB
  • GP145-TrkB
  • trk-B
  • AI848316
  • C030027L06Rik
  • Tkrb
  • trkB
  • RATTRKB1
  • TRKB1
  • Trk-B
  • TrkB
  • neurotrophic tyrosine kinase, receptor, type 2
  • neurotrophic tyrosine kinase, receptor, type 2b
  • NTRK2
  • ntrk2-b
  • ntrk2b
  • ntrk2-a
  • ntrk2
  • Ntrk2
Epitope
AA 32-429
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
21
17
13
2
2
2
2
1
1
1
1
Method Type
Sandwich ELISA
Detection Range
93.8-6000 pg/mL
Minimum Detection Limit
93.8 pg/mL
Application
ELISA
Options
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Catalog No. ABIN921110
$ 400.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
12.357693 ABIN819168 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate 31.25-2000 pg/mL 31.25 pg/mL Log in to see
12.357693 ABIN1116170 Sandwich ELISA Serum, Plasma, Biological Fluids 78.125-5000 pg/mL 78.125 pg/mL Log in to see
12.357693 ABIN2708611 Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Tissue Homogenate 93.8-6000 pg/mL 93.8 pg/mL Log in to see
12.357693 ABIN2114673 Cell Culture Supernatant, Cell Lysate, Tissue Homogenate Log in to see
1 ABIN510833 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids 250-5000 pg/mL 250 pg/mL Log in to see
1 ABIN2685118 Sandwich ELISA Cell Culture Supernatant, Tissue Lysate 93.8-6000 pg/mL 93.8 pg/mL Log in to see 2
1 ABIN1380089 Cell ELISA Log in to see
1 ABIN1380090 Cell ELISA Log in to see
1 ABIN5521325 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 0.156-10 ng/mL 0.156 ng/mL Log in to see
1 ABIN1381568 Cell ELISA Log in to see
1 ABIN1112664 Sandwich ELISA Tissue Lysate, Cell Culture Supernatant 93.8-6000 pg/mL 93.8 pg/mL Log in to see
1 ABIN2042712 Sandwich ELISA 93.8-6000 pg/mL 93.8 pg/mL Log in to see
1 ABIN2113119 Cell ELISA Log in to see
1 ABIN2113118 Cell ELISA Log in to see
1 ABIN1847071 Tissue Homogenate, Biological Fluids 93.8-6000 pg/mL 93.8 pg/mL Log in to see
1 ABIN2113117 Cell ELISA Log in to see
1 ABIN2685117 Cell ELISA Cell Samples, Suspension Cell Culture Log in to see
1 ABIN2949870 78.125-5000 pg/mL 78.125 pg/mL Log in to see
1 ABIN2116384 Sandwich ELISA 93.8-6000 pg/mL 93.8 pg/mL Log in to see
1 ABIN5073086 Sandwich ELISA Plasma, Serum, Tissue Homogenate, Biological Fluids Log in to see

General

Antigen Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) ELISA Kits
Epitope AA 32-429
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(21), (17), (13), (2), (2), (2), (2), (1), (1), (1), (1)
Method Type Sandwich ELISA
Detection Range 93.8-6000 pg/mL
Minimum Detection Limit 93.8 pg/mL
Application ELISA
Pubmed 2 references available
Supplier Log in to see

Product Details TRKB ELISA Kit

Target details Application Details Handling ProductDetails: References for TRKB Kit (ABIN921110) Images
Purpose Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse trkB
Brand PicoKine™
Sample Type Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Detection Method Colorimetric
Analytical Method Quantitative
Specificity Expression system for standard: NSO,C32-H429
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <5pg/mL
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: C32-H429

Target details

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Antigen
Alternative Name NTRK2 (NTRK2 ELISA Kit Abstract)
Background

Protein Function: Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin- 4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2. Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. Isoform GP95-TRKB may also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia. .

Background: TrkB receptor also known as TrkB tyrosine kinase or BDNF/NT-3 growth factors receptor or neurotrophic tyrosine kinase, receptor, type 2 is a protein that in humans is encoded by the NTRK2 gene.[1] TrkB is the high affinity catalytic receptor for several "neurotrophins", which are small protein growth factors that induce the survival and differentiation of distinct cell populations. The TrkB receptor is part of the large family of receptor tyrosine kinases. A "tyrosine kinase" is an enzyme which is capable of adding a phosphate group to certain tyrosines on target proteins, or "substrates".Soppet et al. [2] demonstrated that the gp145 gene product of the TRKB gene is rapidly phosphorylated on tyrosine residues upon exposure to BDNF and NTF3. The standard product used in this kit is recombinant TrkB, a 49.5KDa glycoprotein, C32-H439.

Synonyms: BDNF/NT-3 growth factors receptor,2.7.10.1,GP145-TrkB/GP95-TrkB,Trk-B,Neurotrophic tyrosine kinase receptor type 2,TrkB tyrosine kinase,Ntrk2,Trkb,

Full Gene Name: BDNF/NT-3 growth factors receptor

Cellular Localisation: Cell membrane, Single-pass type I membrane protein . Endosome membrane, Single-pass type I membrane protein . Internalized to endosomes upon ligand-binding.
Gene ID 18212
UniProt P15209
Pathways RTK Signaling, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Skeletal Muscle Fiber Development, Feeding Behaviour, Dicarboxylic Acid Transport

Application Details

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Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Comment

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.

Tissue Specificity: Widely expressed in the central and peripheral nervous system. The different forms are differentially expressed in various cell types. Isoform GP95-TRKB is specifically expressed in glial cells.

Plate Pre-coated
Protocol mouse TrkB ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TrkB has been precoated onto 96-well plates. Standards (NSO,C32-H429) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TrkB is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TrkB amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 6000pg/mL, 3000pg/mL, 1500pg/mL, 750pg/mL, 375pg/mL, 187.5pg/mL, 93.8pg/mL mouse trkB standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, cell lysates or tissue lysates to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse trkB standard solution and each sample is measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 366, Standard deviation: 15.7, CV(%): 4.3
  • Sample 2: n=16, Mean(pg/ml): 2864, Standard deviation: 143.2, CV(%): 5
  • Sample 3: n=16, Mean(pg/ml): 5147, Standard deviation: 329.4, CV(%): 6.4,
  • Sample 1: n=24, Mean(pg/ml): 465, Standard deviation: 25.11, CV(%): 5.4
  • Sample 2: n=24, Mean(pg/ml): 3254, Standard deviation: 198.5, CV(%): 6.1
  • Sample 3: n=24, Mean(pg/ml): 5225, Standard deviation: 386.7, CV(%): 7.4
Restrictions For Research Use only

Handling

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Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

ProductDetails: References for TRKB Kit (ABIN921110)

Product Details TRKB ELISA Kit Target details Application Details Handling Images back to top
Background publications

Nakagawara, Liu, Ikegaki, White, Yamashiro, Nycum, Biegel, Brodeur: "Cloning and chromosomal localization of the human TRK-B tyrosine kinase receptor gene (NTRK2)." in: Genomics, Vol. 25, Issue 2, pp. 538-46, 1995

Soppet, Escandon, Maragos, Middlemas, Reid, Blair, Burton, Stanton, Kaplan, Hunter, Nikolics, Parada: "The neurotrophic factors brain-derived neurotrophic factor and neurotrophin-3 are ligands for the trkB tyrosine kinase receptor." in: Cell, Vol. 65, Issue 5, pp. 895-903, 1991

Images

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Supplier Images
ELISA image for Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) ELISA Kit (ABIN921110) Mouse TrkB PicoKine ELISA Kit standard curve