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Mouse (Murine) TRKB ELISA Kit

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Antigen
Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) ELISA Kits
  • AI848316
  • C030027L06Rik
  • gp145-trkb
  • GP145-TrkB
  • NTRK2
  • ntrk2
  • RATTRKB1
  • si:ch211-220b11.1
  • Tkrb
  • Trk-B
  • trk-B
  • TRKB
  • trkb
  • trkB
  • TrkB
  • trkb-b
  • TRKB1
  • trkB2
  • xtrkb
Epitope
AA 32-429
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
20
14
12
3
3
2
2
2
2
1
1
1
Methode Type
Sandwich ELISA
Detection Range
93.8-6000 pg/mL
Minimum Detection Limit
93.8 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN921110
$ 400.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.30628014 ABIN1116170 Sandwich ELISA Serum, Plasma, Biological Fluids 78.13-5000 pg/mL 78.13 pg/mL Log in to see
0.30628014 ABIN2708611 Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Tissue Homogenate 93.8-6000 pg/mL 93.8 pg/mL Log in to see
0.30628014 ABIN2114673 Cell Culture Supernatant, Cell Lysate, Tissue Homogenate Log in to see
0.0012155735 ABIN819168 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.0012155735 ABIN510833 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids 250-5000 pg/mL 250 pg/mL Log in to see
0.0012155735 ABIN2685118 Sandwich ELISA Cell Culture Supernatant, Tissue Lysate 93.8-6000 pg/mL 93.8 pg/mL Log in to see 2
0.0012155735 ABIN1112664 Sandwich ELISA Tissue Lysate, Cell Culture Supernatant 93.8-6000 pg/mL 93.8 pg/mL Log in to see
0.0012155735 ABIN1380089 Cell ELISA Log in to see
0.0012155735 ABIN1380090 Cell ELISA Log in to see
0.0012155735 ABIN1381568 Cell ELISA Log in to see
0.0012155735 ABIN2042712 Sandwich ELISA 93.8-6000 pg/mL 93.8 pg/mL Log in to see
0.0012155735 ABIN2113118 Cell ELISA Log in to see
0.0012155735 ABIN2113119 Cell ELISA Log in to see
0.0012155735 ABIN2113117 Cell ELISA Log in to see
0.0012155735 ABIN1847071 Tissue Homogenate, Biological Fluids 93.8-6000 pg/mL 93.8 pg/mL Log in to see
0.0012155735 ABIN2685117 Cell ELISA Cell Samples, Suspension Cell Culture Log in to see
0.0012155735 ABIN2093611 Sandwich ELISA Log in to see
0.0012155735 ABIN2949870 78.125-5000 pg/mL 78.125 pg/mL Log in to see
0.0012155735 ABIN2116384 93.8-6000 pg/mL 93.8 pg/mL Log in to see
0.0012155735 ABIN1720545 Log in to see

Top referenced TRKB ELISA Kit for ELISA

Similar TRKB ELISA Kits

Application / Reactivity Mouse (Murine)
ELISA 21 ELISA Kits

General

Antigen Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) ELISA Kits
Epitope AA 32-429
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(20), (14), (12), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 93.8-6000 pg/mL
Minimum Detection Limit 93.8 pg/mL
Application ELISA
Pubmed 2 references available
Supplier Log in to see

Product Details TRKB ELISA Kit

Target details Application Details Handling References for TRKB Kit (ABIN921110) Images
Purpose For quantitative detection of mouse TrkB in cell culture supernates, cell lysates and tissue homogenates.
Brand PicoKine™
Sample Type Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Detection Method Colorimetric
Specificity Natural and recombinant mouse TrkB
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <5pg/mL
Components
  • 96-well plate precoated with anti- mouse TrkBantibod - 1
  • Lyophilized recombinant mouse TrkB standar - 10ng/tubex2
  • Biotinylated anti- mouse TrkB antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: C32-H429

Target details

Product Details TRKB ELISA Kit Application Details Handling References for TRKB Kit (ABIN921110) Images back to top
Antigen
Alternative Name NTRK2 (NTRK2 ELISA Kit Abstract)
Background TrkB receptor also known as TrkB tyrosine kinase or BDNF/NT-3 growth factors receptor or neurotrophic tyrosine kinase, receptor, type 2 is a protein that in humans is encoded by the NTRK2 gene.[1] TrkB is the high affinity catalytic receptor for several "neurotrophins", which are small protein growth factors that induce the survival and differentiation of distinct cell populations. The TrkB receptor is part of the large family of receptor tyrosine kinases. A "tyrosine kinase" is an enzyme which is capable of adding a phosphate group to certain tyrosines on target proteins, or "substrates".Soppet et al. [2] demonstrated that the gp145 gene product of the TRKB gene is rapidly phosphorylated on tyrosine residues upon exposure to BDNF and NTF3. The standard product used in this kit is recombinant TrkB, a 49.5KDa glycoprotein, C32-H439.
Gene ID 18212
UniProt P15209
Pathways RTK Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details TRKB ELISA Kit Target details Handling References for TRKB Kit (ABIN921110) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol mouse TrkB ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TrkB has been precoated onto 96-well plates. Standards (NSO,C32-H429) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TrkB is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TrkB amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 6000pg/mL, 3000pg/mL, 1500pg/mL, 750pg/mL, 375pg/mL, 187.5pg/mL, 93.8pg/mL mouse trkB standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, cell lysates or tissue lysates to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse trkB standard solution and each sample is measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 366, Standard deviation: 15.7, CV(%): 4.3
  • Sample 2: n=16, Mean(pg/ml): 2864, Standard deviation: 143.2, CV(%): 5
  • Sample 3: n=16, Mean(pg/ml): 5147, Standard deviation: 329.4, CV(%): 6.4,
  • Sample 1: n=24, Mean(pg/ml): 465, Standard deviation: 25.11, CV(%): 5.4
  • Sample 2: n=24, Mean(pg/ml): 3254, Standard deviation: 198.5, CV(%): 6.1
  • Sample 3: n=24, Mean(pg/ml): 5225, Standard deviation: 386.7, CV(%): 7.4
Restrictions For Research Use only

Handling

Product Details TRKB ELISA Kit Target details Application Details References for TRKB Kit (ABIN921110) Images back to top
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for TRKB Kit (ABIN921110)

Product Details TRKB ELISA Kit Target details Application Details Handling Images back to top
Background publications

Nakagawara, Liu, Ikegaki, White, Yamashiro, Nycum, Biegel, Brodeur: "Cloning and chromosomal localization of the human TRK-B tyrosine kinase receptor gene (NTRK2)." in: Genomics, Vol. 25, Issue 2, pp. 538-46, 1995

Soppet, Escandon, Maragos, Middlemas, Reid, Blair, Burton, Stanton, Kaplan, Hunter, Nikolics, Parada: "The neurotrophic factors brain-derived neurotrophic factor and neurotrophin-3 are ligands for the trkB tyrosine kinase receptor." in: Cell, Vol. 65, Issue 5, pp. 895-903, 1991

Images

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Supplier Images
ELISA image for TRKB ELISA Kit (Neurotrophic Tyrosine Kinase, Receptor, Type 2) (ABIN921110) Mouse TrkB PicoKine ELISA Kit standard curve