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Mouse (Murine) VEGFR2 ELISA Kit

Recommended VEGFR2 Product (supplied by: Log in to see )

Antigen
Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) ELISA Kits
  • 6130401C07
  • CD309
  • fk52c05
  • flk
  • FLK-1
  • flk-1
  • Flk-1
  • FLK/KDR
  • Flk1
  • flk1
  • FLK1
  • flk1b
  • kdr
  • KDR
  • kdra
  • kdrb
  • Krd-1
  • Ly73
  • si:busm1-205d10.1
  • si:ch211-254j6.1
  • si:ch211-278f21.4
  • sVEGFR-2
  • VEGFR
  • Vegfr-2
  • vegfr-2
  • VEGFR-2
  • VEGFR2
  • vegfr2
  • vegfr4
  • vegr2
  • wu:fc31a09
  • wu:fk52c05
Epitope
AA 20-762
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
32
23
19
6
4
3
3
3
2
2
2
2
2
1
Methode Type
Sandwich ELISA
Detection Range
62.5-4000 pg/mL
Minimum Detection Limit
62.5 pg/mL
Application
ELISA
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Catalog No. ABIN1889306
$ 399.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32693866 ABIN424280 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Biological Fluids 0.15-10 ng/mL 0.15 ng/mL Log in to see
0.32693866 ABIN2708616 Sandwich ELISA Cell Culture Supernatant, Serum 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012117105 ABIN366340 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate 7.8-500 pg/mL 7.8 pg/mL Log in to see
0.0012117105 ABIN628056 Competition ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids Log in to see
0.0012117105 ABIN1030146 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.0012117105 ABIN1380731 Cell ELISA Log in to see
0.0012117105 ABIN1380732 Cell ELISA Log in to see
0.0012117105 ABIN1380730 Cell ELISA Log in to see
0.0012117105 ABIN1380277 Cell ELISA Log in to see
0.0012117105 ABIN1381587 Cell ELISA Log in to see
0.0012117105 ABIN1059797 Serum, Cell Culture Supernatant, Body Fluids, Tissue Homogenate, Plasma Log in to see
0.0012117105 ABIN2703570 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 80-20.000 pg/mL 80 pg/mL Log in to see
0.0012117105 ABIN2684788 Sandwich ELISA Cell Culture Supernatant, Serum 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012117105 ABIN2113917 Cell ELISA Log in to see
0.0012117105 ABIN2113916 Cell ELISA Log in to see
0.0012117105 ABIN2113918 Cell ELISA Log in to see
0.0012117105 ABIN2113915 Cell ELISA Log in to see
0.0012117105 ABIN2113913 Cell ELISA Log in to see
0.0012117105 ABIN2113914 Cell ELISA Log in to see
0.0012117105 ABIN456778 0.312-20 ng/mL 0.312 ng/mL Log in to see

Similar VEGFR2 ELISA Kits

Application / Reactivity Mouse (Murine)
ELISA 24 ELISA Kits

General

Antigen Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) ELISA Kits
Epitope AA 20-762
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(32), (23), (19), (6), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1)
Methode Type Sandwich ELISA
Detection Range 62.5-4000 pg/mL
Minimum Detection Limit 62.5 pg/mL
Application ELISA
Pubmed 2 references available
Supplier Log in to see

Product Details VEGFR2 ELISA Kit

Target details Application Details Handling References for VEGFR2 Kit (ABIN1889306) Images
Purpose For quantitative detection of mouse VEGFR2 in cell culture supernates and serum.
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum
Detection Method Colorimetric
Specificity Natural and recombinant mouse VEGFR2
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <2pg/mL
Components
  • 96-well plate precoated with anti- mouse VEGFR2 antibod - 1
  • Lyophilized recombinant mouse VEGFR2 standar - 10ng/tubex2
  • Biotinylated anti- mouse VEGFR2 antibod - 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC - 130ul(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: A20-E762

Target details

Product Details VEGFR2 ELISA Kit Application Details Handling References for VEGFR2 Kit (ABIN1889306) Images back to top
Antigen
Alternative Name KDR (KDR ELISA Kit Abstract)
Background Vascular endothelial growth factor receptor 2(VEGFR-2) is a VEGF receptor. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It is mapped to 4q12. This gene encodes one of the two receptors of the VEGF. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc. VEGF receptor 2 and the adherens junction act as shear-stress cotransducers, mediating the transduction of shear-stress signals into vascular endothelial cells. In addition to those, VEGFR-2 is also a positive functional marker defining stem cells and distinguishing them from progenitors.
Gene ID 16542
UniProt P35918
Pathways RTK Signaling

Application Details

Product Details VEGFR2 ELISA Kit Target details Handling References for VEGFR2 Kit (ABIN1889306) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol mouse VEGFR2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for VEGFR2 has been precoated onto 96-well plates. Standards(NSO, A20-E762) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for VEGFR2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse VEGFR2 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse VEGFR2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse VEGFR2 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 231, Standard deviation: 8.3, CV(%): 3.6
  • Sample 2: n=16, Mean(pg/ml): 2125, Standard deviation: 87.1, CV(%): 4.1
  • Sample 3: n=16, Mean(pg/ml): 3004, Standard deviation: 156.2, CV(%): 5.2,
  • Sample 1: n=24, Mean(pg/ml): 342, Standard deviation: 14.7, CV(%): 4.3
  • Sample 2: n=24, Mean(pg/ml): 2631, Standard deviation: 147.3, CV(%): 5.6
  • Sample 3: n=24, Mean(pg/ml): 3180, Standard deviation: 200.3, CV(%): 6.3
Restrictions For Research Use only

Handling

Product Details VEGFR2 ELISA Kit Target details Application Details References for VEGFR2 Kit (ABIN1889306) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for VEGFR2 Kit (ABIN1889306)

Product Details VEGFR2 ELISA Kit Target details Application Details Handling Images back to top
Background publications

Shay-Salit, Shushy, Wolfovitz, Yahav, Breviario, Dejana, Resnick: "VEGF receptor 2 and the adherens junction as a mechanical transducer in vascular endothelial cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 14, pp. 9462-7, 2002

Ziegler, Valtieri, Porada, De Maria, Müller, Masella, Gabbianelli, Casella, Pelosi, Bock, Zanjani, Peschle: "KDR receptor: a key marker defining hematopoietic stem cells." in: Science (New York, N.Y.), Vol. 285, Issue 5433, pp. 1553-8, 1999

Images

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Supplier Images
ELISA image for VEGFR2 ELISA Kit (Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase)) (ABIN1889306) Mouse VEGFR2/KDR PicoKine ELISA Kit standard curve