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anti-Human CD247 antibody for Western Blotting

Recommended CD247 Antibody (supplied by: Log in to see )

Antigen
CD247 Molecule (CD247) Antibodies
  • im:7156386
  • LOC100136514
  • CD3Z
  • LOC100136549
  • LOC100305023
  • CD247
  • 4930549J05Rik
  • A430104F18Rik
  • AW552088
  • Cd3
  • Cd3-eta
  • Cd3-zeta
  • Cd3h
  • Cd3z
  • Cd3zeta
  • T3z
  • Tcrk
  • Tcrz
  • CD3-ZETA
  • CD3H
  • CD3Q
  • T3Z
  • TCRZ
  • TCRzeta
  • CD247 antigen like
  • CD3zeta-2
  • CD3zeta-1
  • CD3 zeta
  • CD247 molecule
  • CD247 antigen
  • Cd247 molecule
  • cd247l
  • LOC100136514
  • LOC100136549
  • LOC100305023
  • CD247
  • cd247
  • Cd247
Reactivity
Human, Mouse (Murine)
249
160
39
5
4
4
1
1
1
1
1
Host
Mouse
136
96
21
20
14
3
Clonality
Monoclonal
Conjugate
This CD247 antibody is un-conjugated
25
14
11
8
8
7
7
6
6
6
4
4
4
4
4
4
4
3
3
3
3
2
2
1
Application
Immunoprecipitation (IP), Western Blotting (WB)
186
173
102
45
44
38
29
21
8
4
4
3
2
1
1
1
Options
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Catalog No. ABIN967304
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1870847 ABIN967618 WB Mouse IgG2a, kappa pTyr142 Log in to see K25-407-69 4
3.1870847 ABIN2787935 WB Rabbit C-Term Log in to see Polyclonal
3.1870847 ABIN782349 EIA IHC (p) IP WB Mouse IgG1 AA 1-165 Log in to see 4A12-F6
3.1870847 ABIN967305 IP WB Mouse IgG1 Log in to see 8D3 1
3.1870847 ABIN388169 FACS WB Rabbit Ig AA 2-31, N-Term Log in to see Polyclonal
3.1870847 ABIN388170 WB Rabbit Ig AA 113-141, C-Term Log in to see Polyclonal
3.1870847 ABIN4291760 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
3.1870847 ABIN1531309 ELISA WB Rabbit IgG pTyr142 Log in to see Polyclonal
3.1870847 ABIN1724734 ICC FACS IHC ELISA WB Mouse IgG1 Log in to see 4B10 3
3.1870847 ABIN1532328 ELISA WB Rabbit IgG Log in to see Polyclonal
3.1870847 ABIN1106129 EIA IF IHC (p) WB Mouse IgG1 Log in to see 4B10 2
3.1870847 ABIN2749060 ICC FACS WB Mouse IgG1 pTyr72 Log in to see EM-26 1
3.1870847 ABIN2705768 WB Rabbit Center Log in to see Polyclonal
3.1870847 ABIN4291758 FACS WB Mouse IgG1 pTyr142 Log in to see EM-54
3.1870847 ABIN4291759 FACS WB Mouse IgG1 pTyr111 Log in to see EM-55
3.1870847 ABIN4291761 WB Rabbit IgG C-Term Log in to see Polyclonal
3.1870847 ABIN1871602 IF IHC WB Rabbit IgG Log in to see Polyclonal
3.1870847 ABIN4291754 ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 4B10
3.1870847 ABIN1981867 FACS WB Mouse IgG1 pTyr153 Log in to see EM-17
3.1870847 ABIN4291755 CyTOF FACS ICC IF IP WB Armenian Hamster IgG Log in to see H146-968

Top referenced anti-CD247 antibody for Western Blotting

Similar anti-CD247 Antibodies

Application / Reactivity Mouse (Murine) Human
Cytometry by Time of Flight (CyTOF) 1 Antibodies 1 Antibodies
ELISA 22 Antibodies 94 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 4 Antibodies
Flow Cytometry (FACS) 106 Antibodies 135 Antibodies
Functional Studies (Func) 1 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 24 Antibodies
Immunofluorescence (IF) 17 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies
Immunohistochemistry (IHC) 16 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies
Immunoprecipitation (IP) 16 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Western Blotting (WB) 109 Antibodies

General

Antigen CD247 Molecule (CD247) Antibodies
Reactivity Human, Mouse (Murine)
(249), (160), (39), (5), (4), (4), (1), (1), (1), (1), (1)
Host Mouse
(136), (96), (21), (20), (14), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD247 antibody is un-conjugated
(25), (14), (11), (8), (8), (7), (7), (6), (6), (6), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (1)
Application Immunoprecipitation (IP), Western Blotting (WB)
(186), (173), (102), (45), (44), (38), (29), (21), (8), (4), (4), (3), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD247 Antibody

Target Details CD247 Application Details Handling References for anti-CD247 Antibody (ABIN967304) Images
Brand BD Pharmingen™
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human CD3zeta synthetic peptide
Clone 8D3
Isotype IgG1

Target Details CD247

Product Details anti-CD247 Antibody Application Details Handling References for anti-CD247 Antibody (ABIN967304) Images back to top
Antigen
Alternative Name CD247 (CD247 Antibody Abstract)
Background The T-cell receptor (TCR), expressed by thymus-derived lymphocytes, is a multi-component complex responsible for recognizing antigen in the context of MHC molecules. Ti is the antigen-specific binding component of the TCR. On the majority of peripheral T lymphocytes Ti is present as a disulfide-linked alpha-beta heterodimer. A minor fraction express Ti as a gamma-delta heterodimer. The Ti subunits are Ig-like, each containing V and C regions. Ti is noncovalently associated with an invariant set of molecules referred to as the CD3 subunits. CD3 appears early in thymocyte differentiation and remains expressed on all mature T lymphocytes. In the mouse, it consists of five chains, gamma (gamma), delta (delta), epsilon (epsilon), zeta (zeta), and p21. The relative masses of the mouse CD3 chains are 21, 28, 26, 32 (dimer), and 21 kDa respectively. The 8D3 antibody recognizes the zeta chain of the human and mouse T cell antigen receptor-associated CD3 complex. It reacts with 16 kDa unphosphorylated monomers and 32 kDa unphosphorylated dimers. It does not appear to recognize 21 kDa phosphorylated monomers or 42 kDa phosphorylated dimers. A synthetic peptide corresponding to a region of human CD3zeta was used as immunogen.
Synonyms: CD3zeta chain
Molecular Weight 16 kDa, 32 kDa
Pathways TCR Signaling, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD247 Antibody Target Details CD247 Handling References for anti-CD247 Antibody (ABIN967304) Images back to top
Application Notes Jurkat control lysate [50 µg (1 µg/µl) is provided as a Western blot positive control (Comp. 51-16526N, store lysate at -20°C). Additional control lysate (ABIN968537) is sold separately as a ready-to-use western blot control.
Comment

Related Products: ABIN968537

Restrictions For Research Use only

Handling

Product Details anti-CD247 Antibody Target Details CD247 Application Details References for anti-CD247 Antibody (ABIN967304) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.

References for anti-CD247 Antibody (ABIN967304)

Product Details anti-CD247 Antibody Target Details CD247 Application Details Handling Images back to top
Background publications

Hussey, Clayton, Diener, McConkey, Howard, Rodewald, DAdamio, Dallenbach, Stein, Schmidt: "Overexpression of CD3 eta during thymic development does not alter the negative selection process." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 4, pp. 1183-94, 1993

Images

Product Details anti-CD247 Antibody Target Details CD247 Application Details Handling References for anti-CD247 Antibody (ABIN967304) back to top
Supplier Images
Western Blotting (WB) image for anti-CD247 antibody (CD247 Molecule) (ABIN967304) Western blot analysis of CD3zeta. Lysate from Jurkat cells was probed with anti-CD3ze...