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anti-Human CD247 antibody for Western Blotting

Recommended CD247 Antibody (supplied by: Log in to see )

Antigen
CD247 Molecule (CD247) Antibodies
  • 4930549J05Rik
  • A430104F18Rik
  • AW552088
  • Cd3
  • Cd3-eta
  • CD3-ZETA
  • Cd3-zeta
  • CD3H
  • Cd3h
  • CD3Q
  • Cd3z
  • CD3Z
  • Cd3zeta
  • CD247
  • im:7156386
  • LOC100136514
  • LOC100136549
  • LOC100305023
  • T3Z
  • T3z
  • Tcrk
  • Tcrz
  • TCRZ
  • TCRzeta
Reactivity
Human
268
121
29
4
2
2
1
1
Host
Mouse
155
99
15
11
1
Clonality
Monoclonal
Conjugate
This CD247 antibody is un-conjugated
30
20
10
7
6
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
198
136
106
57
46
45
33
21
12
8
3
2
1
1
Supplier
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Catalog No. ABIN1724734
$ 393.80
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13341247 ABIN967304 IP WB Mouse IgG1 Log in to see 8D3 1
0.13341247 ABIN967618 WB Mouse IgG2a, kappa pTyr142 Log in to see K25-407-69 4
0.13341247 ABIN967305 IP WB Mouse IgG1 Log in to see 8D3 1
0.13341247 ABIN1531309 ELISA WB Rabbit IgG pTyr142 Log in to see Polyclonal
0.13341247 ABIN782349 EIA IHC (p) IP WB Mouse IgG1 AA 1-165 Log in to see 4A12-F6
0.13341247 ABIN388169 FACS WB Rabbit Ig AA 2-31, N-Term Log in to see Polyclonal
0.13341247 ABIN388170 WB Rabbit Ig AA 113-141, C-Term Log in to see Polyclonal
0.13341247 ABIN1532328 ELISA WB Rabbit IgG Log in to see Polyclonal
0.13341247 ABIN2787935 WB Rabbit C-Term Log in to see Polyclonal
0.13341247 ABIN1106129 EIA IF IHC (p) WB Mouse IgG1 Log in to see 4B10 2
0.13341247 ABIN2749060 ICC FACS WB Mouse IgG1 pTyr72 Log in to see EM-26 1
0.13341247 ABIN2705768 WB Rabbit Center Log in to see Polyclonal
0.13341247 ABIN1871602 IHC WB Rabbit IgG Log in to see Polyclonal
0.13341247 ABIN2167720 FACS IF IHC ELISA WB Mouse IgG1 Log in to see 4B10
0.13341247 ABIN2163416 ELISA WB Rabbit IgG Tyr142 Log in to see Polyclonal
0.13341247 ABIN2163417 ELISA WB Rabbit IgG Tyr42 Log in to see Polyclonal
0.13341247 ABIN951241 EIA FACS IF WB Hamster IgG Log in to see H146-968
0.13341247 ABIN1981867 FACS WB Mouse IgG1 pTyr153 Log in to see EM-17
0.13341247 ABIN3022813 IHC WB Rabbit IgG Log in to see Polyclonal
0.13341247 ABIN2749059 FACS WB Mouse IgG1 pTyr111, pTyr123 Log in to see EM-55

Top referenced anti-CD247 antibody for Western Blotting

Similar anti-CD247 Antibodies

Application / Reactivity Human
ELISA 98 Antibodies
Enzyme Immunoassay (EIA) 8 Antibodies
Flow Cytometry (FACS) 129 Antibodies
Functional Studies (Func) 2 Antibodies
Immunocytochemistry (ICC) 46 Antibodies
Immunofluorescence (IF) 58 Antibodies
Immunohistochemistry (IHC) 34 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 11 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 21 Antibodies
Immunoprecipitation (IP) 44 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Microcytotoxicity Assay (MCA) 1 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 187 Antibodies

General

Antigen CD247 Molecule (CD247) Antibodies
Reactivity Human
(268), (121), (29), (4), (2), (2), (1), (1)
Host Mouse
(155), (99), (15), (11), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD247 antibody is un-conjugated
(30), (20), (10), (7), (6), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(198), (136), (106), (57), (46), (45), (33), (21), (12), (8), (3), (2), (1), (1)
Pubmed 3 references available
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Product Details anti-CD247 Antibody

Target Details CD247 Application Details Handling References for anti-CD247 Antibody (ABIN1724734) Images
Immunogen Purified recombinant fragment of human CD247 expressed in E. Coli.
Clone 4B10
Isotype IgG1

Target Details CD247

Product Details anti-CD247 Antibody Application Details Handling References for anti-CD247 Antibody (ABIN1724734) Images back to top
Antigen
Alternative Name CD247 (CD247 Antibody Abstract)
Background The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Molecular Weight 18 kDa
Gene ID 919
HGNC 919
Pathways TCR Signaling

Application Details

Product Details anti-CD247 Antibody Target Details CD247 Handling References for anti-CD247 Antibody (ABIN1724734) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
ICC: 1/200 - 1/1000
FCM: 1/200 - 1/400
Restrictions For Research Use only

Handling

Product Details anti-CD247 Antibody Target Details CD247 Application Details References for anti-CD247 Antibody (ABIN1724734) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-CD247 Antibody (ABIN1724734)

Product Details anti-CD247 Antibody Target Details CD247 Application Details Handling Images back to top
Product cited in:

Tao, Wollscheid, OBrien, Eng, Li, Bodenmiller, Watts, Hood, Aebersold: "Quantitative phosphoproteome analysis using a dendrimer conjugation chemistry and tandem mass spectrometry." in: Nature methods, Vol. 2, Issue 8, pp. 591-8, 2005

Nambiar, Fisher, Warke, Krishnan, Mitchell, Delaney, Tsokos: "Reconstitution of deficient T cell receptor zeta chain restores T cell signaling and augments T cell receptor/CD3-induced interleukin-2 production in patients with systemic lupus erythematosus." in: Arthritis and rheumatism, Vol. 48, Issue 7, pp. 1948-55, 2003

Dumont, Blanchard, Di Bartolo, Lezot, Dufour, Jauliac, Hivroz: "TCR/CD3 down-modulation and zeta degradation are regulated by ZAP-70." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 4, pp. 1705-12, 2002

Images

Product Details anti-CD247 Antibody Target Details CD247 Application Details Handling References for anti-CD247 Antibody (ABIN1724734) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD247 antibody (CD247 Molecule) (ABIN1724734) anti-CD247 Molecule (CD247) antibody
Immunocytochemistry (ICC) image for anti-CD247 antibody (CD247 Molecule) (ABIN1724734) anti-CD247 Molecule (CD247) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-CD247 antibody (CD247 Molecule) (ABIN1724734) anti-CD247 Molecule (CD247) antibody (Image 3)
Western Blotting (WB) image for anti-CD247 antibody (CD247 Molecule) (ABIN1724734) anti-CD247 Molecule (CD247) antibody (Image 4)