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anti-Human CD247 antibody for Western Blotting

Recommended CD247 Antibody (supplied by: Log in to see )

Antigen
CD247 Molecule (CD247) Antibodies
  • im:7156386
  • LOC100136514
  • CD3Z
  • LOC100136549
  • LOC100305023
  • CD247
  • 4930549J05Rik
  • A430104F18Rik
  • AW552088
  • Cd3
  • Cd3-eta
  • Cd3-zeta
  • Cd3h
  • Cd3z
  • Cd3zeta
  • T3z
  • Tcrk
  • Tcrz
  • CD3-ZETA
  • CD3H
  • CD3Q
  • T3Z
  • TCRZ
  • TCRzeta
  • CD247 antigen like
  • CD3zeta-2
  • CD3zeta-1
  • CD3 zeta
  • CD247 molecule
  • CD247 antigen
  • Cd247 molecule
  • cd247l
  • LOC100136514
  • LOC100136549
  • LOC100305023
  • CD247
  • cd247
  • Cd247
Reactivity
Human
243
156
39
5
3
3
1
1
Host
Mouse
134
93
21
20
14
2
Clonality
Monoclonal
Conjugate
This CD247 antibody is un-conjugated
25
14
11
8
8
7
7
6
6
6
4
4
4
4
4
4
4
3
3
3
3
2
2
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
180
169
100
45
43
37
31
21
9
5
4
3
2
1
1
1
Options
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Catalog No. ABIN1724734
$ 393.80
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0726016 ABIN967618 WB Mouse IgG2a, kappa pTyr142 Log in to see K25-407-69 4
3.0726016 ABIN967304 IP WB Mouse IgG1 Log in to see 8D3 1
3.0726016 ABIN388170 WB Rabbit Ig AA 113-141, C-Term Log in to see Polyclonal
3.0726016 ABIN388169 FACS WB Rabbit Ig AA 2-31, N-Term Log in to see Polyclonal
3.0726016 ABIN967305 IP WB Mouse IgG1 Log in to see 8D3 1
3.0726016 ABIN4291760 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
3.0726016 ABIN1531309 ELISA WB Rabbit IgG pTyr142 Log in to see Polyclonal
3.0726016 ABIN782349 EIA IHC (p) IP WB Mouse IgG1 AA 1-165 Log in to see 4A12-F6
3.0726016 ABIN1532328 ELISA WB Rabbit IgG Log in to see Polyclonal
3.0726016 ABIN1106129 EIA IF IHC (p) WB Mouse IgG1 Log in to see 4B10 2
3.0726016 ABIN2749060 ICC FACS WB Mouse IgG1 pTyr72 Log in to see EM-26 1
3.0726016 ABIN2787935 WB Rabbit C-Term Log in to see Polyclonal
3.0726016 ABIN2705768 WB Rabbit Center Log in to see Polyclonal
3.0726016 ABIN4291759 FACS WB Mouse IgG1 pTyr111 Log in to see EM-55
3.0726016 ABIN4291761 WB Rabbit IgG C-Term Log in to see Polyclonal
3.0726016 ABIN4291758 FACS WB Mouse IgG1 pTyr142 Log in to see EM-54
3.0726016 ABIN1871602 IF IHC WB Rabbit IgG Log in to see Polyclonal
3.0726016 ABIN4291754 ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 4B10
3.0726016 ABIN1981867 FACS WB Mouse IgG1 pTyr153 Log in to see EM-17
3.0726016 ABIN4291755 CyTOF FACS ICC IF IP WB Armenian Hamster IgG Log in to see H146-968

Top referenced anti-CD247 antibody for Western Blotting

Similar anti-CD247 Antibodies

Application / Reactivity Human
Cytometry by Time of Flight (CyTOF) 1 Antibodies
ELISA 93 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 132 Antibodies
Functional Studies (Func) 2 Antibodies
Immunocytochemistry (ICC) 35 Antibodies
Immunofluorescence (IF) 45 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 4 Antibodies
Immunohistochemistry (IHC) 35 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 9 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 21 Antibodies
Immunoprecipitation (IP) 25 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Microcytotoxicity Assay (MCA) 1 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 170 Antibodies

General

Antigen CD247 Molecule (CD247) Antibodies
Reactivity Human
(243), (156), (39), (5), (3), (3), (1), (1)
Host Mouse
(134), (93), (21), (20), (14), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD247 antibody is un-conjugated
(25), (14), (11), (8), (8), (7), (7), (6), (6), (6), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(180), (169), (100), (45), (43), (37), (31), (21), (9), (5), (4), (3), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-CD247 Antibody

Target Details CD247 Application Details Handling References for anti-CD247 Antibody (ABIN1724734) Images
Immunogen Purified recombinant fragment of human CD247 expressed in E. Coli.
Clone 4B10
Isotype IgG1

Target Details CD247

Product Details anti-CD247 Antibody Application Details Handling References for anti-CD247 Antibody (ABIN1724734) Images back to top
Antigen
Alternative Name CD247 (CD247 Antibody Abstract)
Background The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Molecular Weight 18 kDa
Gene ID 919
HGNC 919
Pathways TCR Signaling

Application Details

Product Details anti-CD247 Antibody Target Details CD247 Handling References for anti-CD247 Antibody (ABIN1724734) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
ICC: 1/200 - 1/1000
FCM: 1/200 - 1/400
Restrictions For Research Use only

Handling

Product Details anti-CD247 Antibody Target Details CD247 Application Details References for anti-CD247 Antibody (ABIN1724734) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-CD247 Antibody (ABIN1724734)

Product Details anti-CD247 Antibody Target Details CD247 Application Details Handling Images back to top
Product cited in:

Tao, Wollscheid, OBrien, Eng, Li, Bodenmiller, Watts, Hood, Aebersold: "Quantitative phosphoproteome analysis using a dendrimer conjugation chemistry and tandem mass spectrometry." in: Nature methods, Vol. 2, Issue 8, pp. 591-8, 2005

Nambiar, Fisher, Warke, Krishnan, Mitchell, Delaney, Tsokos: "Reconstitution of deficient T cell receptor zeta chain restores T cell signaling and augments T cell receptor/CD3-induced interleukin-2 production in patients with systemic lupus erythematosus." in: Arthritis and rheumatism, Vol. 48, Issue 7, pp. 1948-55, 2003

Dumont, Blanchard, Di Bartolo, Lezot, Dufour, Jauliac, Hivroz: "TCR/CD3 down-modulation and zeta degradation are regulated by ZAP-70." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 4, pp. 1705-12, 2002

Images

Product Details anti-CD247 Antibody Target Details CD247 Application Details Handling References for anti-CD247 Antibody (ABIN1724734) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD247 antibody (CD247 Molecule) (ABIN1724734) anti-CD247 Molecule (CD247) antibody
Immunocytochemistry (ICC) image for anti-CD247 antibody (CD247 Molecule) (ABIN1724734) anti-CD247 Molecule (CD247) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-CD247 antibody (CD247 Molecule) (ABIN1724734) anti-CD247 Molecule (CD247) antibody (Image 3)
Western Blotting (WB) image for anti-CD247 antibody (CD247 Molecule) (ABIN1724734) anti-CD247 Molecule (CD247) antibody (Image 4)