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anti-Human CD247 antibody for Western Blotting

Recommended CD247 Antibody (supplied by: Log in to see )

Antigen
CD247 Molecule (CD247) Antibodies
  • 4930549J05Rik
  • A430104F18Rik
  • AW552088
  • Cd3
  • Cd3-eta
  • CD3-ZETA
  • Cd3-zeta
  • CD3H
  • Cd3h
  • CD3Q
  • Cd3z
  • CD3Z
  • Cd3zeta
  • CD247
  • im:7156386
  • LOC100136514
  • LOC100136549
  • LOC100305023
  • T3Z
  • T3z
  • Tcrk
  • Tcrz
  • TCRZ
  • TCRzeta
Epitope
pTyr142
33
27
23
23
22
14
14
5
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
266
121
28
4
2
2
1
1
Host
Mouse
155
97
15
11
1
Clonality
Monoclonal
Conjugate
This CD247 antibody is un-conjugated
31
17
10
7
6
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
1
Application
Western Blotting (WB)
196
136
110
56
49
45
35
21
12
8
3
2
1
1
Supplier
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Catalog No. ABIN967618
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12935057 ABIN1724734 ICC FACS IHC ELISA WB Mouse IgG1 Log in to see 4B10 3
0.12935057 ABIN967304 IP WB Mouse IgG1 Log in to see 8D3 1
0.12935057 ABIN782349 EIA IHC (p) IP WB Mouse IgG1 AA 1-165 Log in to see 4A12-F6
0.12935057 ABIN967305 IP WB Mouse IgG1 Log in to see 8D3 1
0.12935057 ABIN1531309 ELISA WB Rabbit IgG pTyr142 Log in to see Polyclonal
0.12935057 ABIN388170 WB Rabbit Ig AA 113-141, C-Term Log in to see Polyclonal
0.12935057 ABIN388169 FACS WB Rabbit Ig AA 2-31, N-Term Log in to see Polyclonal
0.12935057 ABIN1532328 ELISA WB Rabbit IgG Log in to see Polyclonal
0.12935057 ABIN2787935 WB Rabbit C-Term Log in to see Polyclonal
0.12935057 ABIN1106129 EIA IF IHC (p) WB Mouse IgG1 Log in to see 4B10 2
0.12935057 ABIN2749060 ICC FACS WB Mouse IgG1 pTyr72 Log in to see EM-26 1
0.12935057 ABIN2705768 WB Rabbit Center Log in to see Polyclonal
0.12935057 ABIN1871602 IHC WB Rabbit IgG Log in to see Polyclonal
0.12935057 ABIN2163417 ELISA WB Rabbit IgG Tyr42 Log in to see Polyclonal
0.12935057 ABIN2163416 ELISA WB Rabbit IgG Tyr142 Log in to see Polyclonal
0.12935057 ABIN2167720 FACS IF IHC ELISA WB Mouse IgG1 Log in to see 4B10
0.12935057 ABIN1981867 FACS WB Mouse IgG1 pTyr153 Log in to see EM-17
0.12935057 ABIN951241 EIA FACS IF WB Hamster IgG Log in to see H146-968
0.12935057 ABIN3022813 IHC WB Rabbit IgG Log in to see Polyclonal
0.12935057 ABIN4291760 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 1

Top referenced anti-CD247 antibody for Western Blotting

Similar anti-CD247 Antibodies

Application / Reactivity Human
ELISA 101 Antibodies
Enzyme Immunoassay (EIA) 8 Antibodies
Flow Cytometry (FACS) 127 Antibodies
Functional Studies (Func) 2 Antibodies
Immunocytochemistry (ICC) 47 Antibodies
Immunofluorescence (IF) 56 Antibodies
Immunohistochemistry (IHC) 35 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 11 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 21 Antibodies
Immunoprecipitation (IP) 43 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Microcytotoxicity Assay (MCA) 1 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 184 Antibodies

General

Antigen CD247 Molecule (CD247) Antibodies
Epitope pTyr142
(33), (27), (23), (23), (22), (14), (14), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(266), (121), (28), (4), (2), (2), (1), (1)
Host Mouse
(155), (97), (15), (11), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD247 antibody is un-conjugated
(31), (17), (10), (7), (6), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (1)
Application Western Blotting (WB)
(196), (136), (110), (56), (49), (45), (35), (21), (12), (8), (3), (2), (1), (1)
Pubmed 4 references available
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Product Details anti-CD247 Antibody

Target Details CD247 Application Details Handling References for anti-CD247 Antibody (ABIN967618) Images
Brand BD Pharmingen™
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated Human CD3zeta Peptide
Clone K25-407-69
Isotype IgG2a, kappa

Target Details CD247

Product Details anti-CD247 Antibody Application Details Handling References for anti-CD247 Antibody (ABIN967618) Images back to top
Antigen
Alternative Name CD247 (CD3z) (CD247 Antibody Abstract)
Background The T cell receptor (TCR), expressed by thymocytes and T lymphocytes, is a multi-component cell-surface complex responsible for recognizing antigen in the context of MHC molecules. The antigen-specific binding component of the TCR, Ti, is a heterodimer of the variable Ig-like subunits a and b or g and d. Ti is non-covalently associated with an invariant set of molecules referred to as the CD3 polypeptides, g, d, e, and zeta. The CD3 z polypeptide (CD3z) was named CD247 at the 7th Human Leukocyte Differentiation Antigens Workshop. CD3 appears early in thymocyte differentiation and remains expressed on all mature T lymphocytes. After antigen recognition by the TCR, CD3z is the primary intracellular signal transducing subunit. It contains three ITAMs (Immunoreceptor Tyrosine-based Activation Motifs), each of which contains a pair of tyrosine residues that are phosphorylated by Lck and Fyn and are required for signal propagation. The molecular weight of CD3z is 16 kDa, and it is also observed as 32-kDa homodimers or as heterodimers with the g chain of Fc receptors. Upon phosphorylation, the CD3z monomer undergoes an apparent shift in electrophoretic mobility up to 21 kDa. The K25-407.69 monoclonal antibody recognizes the phosphorylated tyrosine 142 (pY142) in the third ITAM domain of human CD3z (CD247).
Synonyms: CD3zeta
Molecular Weight 21 kDa
Pathways TCR Signaling

Application Details

Product Details anti-CD247 Antibody Target Details CD247 Handling References for anti-CD247 Antibody (ABIN967618) Images back to top
Comment

Related Products: ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-CD247 Antibody Target Details CD247 Application Details References for anti-CD247 Antibody (ABIN967618) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-CD247 Antibody (ABIN967618)

Product Details anti-CD247 Antibody Target Details CD247 Application Details Handling Images back to top
Product cited in:

Salomon, Ficarro, Brill, Brinker, Phung, Ericson, Sauer, Brock, Horn, Schultz, Peters: "Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 2, pp. 443-8, 2003

Ernst, Shih: "CD3 complex." in: Journal of biological regulators and homeostatic agents, Vol. 14, Issue 3, pp. 226-9, 2001

Kersh, Shaw, Allen: "Fidelity of T cell activation through multistep T cell receptor zeta phosphorylation." in: Science (New York, N.Y.), Vol. 281, Issue 5376, pp. 572-5, 1998

Alberola-Ila, Takaki, Kerner, Perlmutter: "Differential signaling by lymphocyte antigen receptors." in: Annual review of immunology, Vol. 15, pp. 125-54, 1997

Images

Product Details anti-CD247 Antibody Target Details CD247 Application Details Handling References for anti-CD247 Antibody (ABIN967618) back to top
Supplier Images
Western Blotting (WB) image for anti-CD247 antibody (CD247 Molecule) (pTyr142) (ABIN967618) Western blot analysis of CD3z (CD247) (pY142) in human T lymphocytes. Lysates from co...