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anti-Mouse (Murine) CD247 antibody for Immunocytochemistry

Recommended CD247 Antibody (supplied by: Log in to see )

Antigen
CD247 Molecule (CD247) Antibodies
  • 4930549J05Rik
  • A430104F18Rik
  • AW552088
  • Cd3
  • Cd3-eta
  • CD3-ZETA
  • Cd3-zeta
  • CD3H
  • Cd3h
  • CD3Q
  • Cd3z
  • CD3Z
  • Cd3zeta
  • CD247
  • im:7156386
  • LOC100136514
  • LOC100136549
  • LOC100305023
  • T3Z
  • T3z
  • Tcrk
  • Tcrz
  • TCRZ
  • TCRzeta
Epitope
pTyr72
34
28
23
23
22
14
14
5
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
268
120
29
4
2
2
1
1
Host
Mouse
156
99
14
11
1
Clonality
Monoclonal
Conjugate
This CD247 antibody is un-conjugated
29
21
10
7
6
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Western Blotting (WB)
198
136
107
57
46
45
34
21
12
8
3
2
1
1
Supplier
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Catalog No. ABIN2749060
€ 220.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010194078 ABIN343660 ICC WB Rabbit IgG Log in to see Polyclonal
0.0010194078 ABIN3199737 FACS ICC WB Mouse IgG1 Tyr72 Log in to see EM-26
0.0010194078 ABIN4291764 FACS ICC IF IP WB PE Armenian Hamster IgG Log in to see H146-968
0.0010194078 ABIN4291765 FACS ICC IF IP WB PerCP Armenian Hamster IgG Log in to see H146-968
0.0010194078 ABIN4291766 FACS ICC IF IP WB Allophycocyanin Armenian Hamster IgG Log in to see H146-968
0.0010194078 ABIN4291755 FACS ICC IF IP WB Armenian Hamster IgG Log in to see H146-968
0.0010194078 ABIN4291762 FACS ICC IF IP WB DyLight 350 Armenian Hamster IgG Log in to see H146-968
0.0010194078 ABIN4291767 FACS ICC IF IP WB DyLight 755 Armenian Hamster IgG Log in to see H146-968
0.0010194078 ABIN4291768 FACS ICC IF IP WB DyLight 680 Armenian Hamster IgG Log in to see H146-968
0.0010194078 ABIN4291769 FACS ICC IF IP WB DyLight 488 Armenian Hamster IgG Log in to see H146-968
0.0010194078 ABIN4291770 FACS ICC IF IP WB DyLight 405LS Armenian Hamster IgG Log in to see H146-968
0.0010194078 ABIN4291771 FACS ICC IF IP WB Alexa Fluor 405 Armenian Hamster IgG Log in to see H146-968
0.0010194078 ABIN4291772 FACS ICC IF IP WB Alexa Fluor 488 Armenian Hamster IgG Log in to see H146-968
0.0010194078 ABIN4291773 FACS ICC IF IP WB Alexa Fluor 647 Armenian Hamster IgG Log in to see H146-968
0.0010194078 ABIN4291774 FACS ICC IF IP WB Alexa Fluor 700 Armenian Hamster IgG Log in to see H146-968
0.0010194078 ABIN4291763 FACS ICC IF IP WB DyLight 405 Armenian Hamster IgG Log in to see H146-968
0.0010194078 ABIN1112854 ICC WB Rabbit IgG Log in to see Polyclonal
0.0010194078 ABIN3037088 ICC FACS IF WB Mouse IgG1 pTyr72 Log in to see
0.0010194078 ABIN1854858 ICC IF WB Rabbit IgG C-Term Log in to see Polyclonal
0.0010194078 ABIN2973323 ICC IF IHC WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-CD247 antibody for Immunocytochemistry

Similar anti-CD247 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 21 Antibodies 98 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies 8 Antibodies
Flow Cytometry (FACS) 49 Antibodies 130 Antibodies
Immunocytochemistry (ICC) 22 Antibodies 47 Antibodies
Immunofluorescence (IF) 21 Antibodies 59 Antibodies
Immunohistochemistry (IHC) 9 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies
Immunoprecipitation (IP) 24 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Western Blotting (WB) 108 Antibodies
Functional Studies (Func) 2 Antibodies

General

Antigen CD247 Molecule (CD247) Antibodies
Epitope pTyr72
(34), (28), (23), (23), (22), (14), (14), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(268), (120), (29), (4), (2), (2), (1), (1)
Host Mouse
(156), (99), (14), (11), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD247 antibody is un-conjugated
(29), (21), (10), (7), (6), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Western Blotting (WB)
(198), (136), (107), (57), (46), (45), (34), (21), (12), (8), (3), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD247 Antibody

Target Details CD247 Application Details Handling References for anti-CD247 Antibody (ABIN2749060) Images
Specificity The mouse monoclonal antibody EM-26 recognizes phosphorylated tyrosine 72 of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.
Purification purified.
Immunogen A phospho specific peptide corresponding to the amino acids surrounding tyrosine 72 of mouse CD3 zeta linked to KLH
Clone EM-26
Isotype IgG1

Target Details CD247

Product Details anti-CD247 Antibody Application Details Handling References for anti-CD247 Antibody (ABIN2749060) Images back to top
Antigen
Alternative Name CD3 zeta (CD247 Antibody Abstract)
Background CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta (CD247). These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
Pathways TCR Signaling

Application Details

Product Details anti-CD247 Antibody Target Details CD247 Handling References for anti-CD247 Antibody (ABIN2749060) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD247 Antibody Target Details CD247 Application Details References for anti-CD247 Antibody (ABIN2749060) Images back to top
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

References for anti-CD247 Antibody (ABIN2749060)

Product Details anti-CD247 Antibody Target Details CD247 Application Details Handling Images back to top
Product cited in:

Dopfer, Schöpf, Louis-Dit-Sully, Dengler, Höhne, Klescová, Prouza, Suchanek, Reth, Schamel: "Analysis of novel phospho-ITAM specific antibodies in a S2 reconstitution system for TCR-CD3 signalling." in: Immunology letters, Vol. 130, Issue 1-2, pp. 43-50, 2010

Images

Product Details anti-CD247 Antibody Target Details CD247 Application Details Handling References for anti-CD247 Antibody (ABIN2749060) back to top
Supplier Images
Western Blotting (WB) image for anti-CD247 antibody (CD247 Molecule) (pTyr72) (ABIN2749060) Reactivity of the monoclonal antibodies EM-26 (anti-CD3 zeta phospho-Tyr72) and EM-17...
Immunofluorescence (IF) image for anti-CD247 antibody (CD247 Molecule) (pTyr72) (ABIN2749060) Detection of phosphorylated CD3 zeta (; light blue) in immunological synapse formed b...