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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Immunofluorescence (fixed cells) (IF/ICC)||4 Antibodies||3 Antibodies||2 Antibodies|
|Cytometry by Time of Flight (CyTOF)||1 Antibodies||1 Antibodies|
|Western Blotting (WB)||181 Antibodies||110 Antibodies||32 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Microcytotoxicity Assay (MCA)||1 Antibodies|
|Intracellular Staining (ICS)||3 Antibodies||3 Antibodies||3 Antibodies|
|Immunoprecipitation (IP)||24 Antibodies||16 Antibodies||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||24 Antibodies||2 Antibodies||2 Antibodies|
|Immunohistochemistry (IHC)||35 Antibodies|
|Antigen||CD247 Molecule (CD247) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This CD247 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
|Supplier||Log in to see|
Product Details anti-CD247 AntibodyTarget Details CD247 Application Details Handling Images
This antibody recognizes CD3 zeta.
Cellular Localization: Membrane.
Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.
|Immunogen||A synthetic peptide corresponding from near N-terminus of the zeta chain of Human CD3 protein.|
Target Details CD247Product Details anti-CD247 Antibody Application Details Handling Images back to top
|Alternative Name||CD247 / CD3Z (CD247 Antibody Abstract)|
|Background||CD3 (Cluster of Differentiation 3) consists of five invariant polypeptide chains that associate to form three dimmers: a heterodimer of gamma and epsilon chains, a heterodimer of delta and epsilon chains and a homodimer of two zeta chains or a heterodimer of zeta and eta chains. The zeta chain contains three consecutive copies of the same motif.Synonyms: CD3 zeta chain, T-cell receptor T3 zeta chain, T-cell surface glycoprotein CD3 zeta chain, T3Z, TCRZ|
|Molecular Weight||approx. 20 kDa|
|Gene ID||919, 9606|
|Research Area||CD Antigens, Surface Receptors of Immune Cells|
|Pathways||TCR Signaling, CXCR4-mediated Signaling Events|
Application DetailsProduct Details anti-CD247 Antibody Target Details CD247 Handling Images back to top
Western Blotting. Immunoprecipitation. Immunohistochemistry on paraffin sections: 1/200 for 10 min at RT, Staining offormalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Positive Control: Jurkat cells and tonsil
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD247 Antibody Target Details CD247 Application Details Images back to top
|Buffer||10 mM PBS, pH 7.4 with 1 % BSA and 0.09 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||DO NOT FREEZE!!|
|Storage Comment||Store the antibody undiluted at 2-8 °C.|