anti-Human CD28 antibody for Agonist

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • Tp44
  • CD28RNA
  • CD28 molecule
  • CD28 antigen
  • Cd28 molecule
  • CD28
  • Cd28
Epitope
AA 19-152
17
16
15
12
11
11
10
10
8
7
6
4
4
4
3
3
3
3
3
1
1
1
1
1
Reactivity
Human
494
410
103
70
67
42
41
34
27
23
15
7
3
3
3
2
1
1
1
Host
Mouse
526
199
92
69
40
29
12
1
Clonality
Monoclonal
Conjugate
This CD28 antibody is un-conjugated
112
95
74
37
21
20
19
19
18
17
17
13
13
13
13
12
12
12
8
7
6
6
6
6
6
5
4
3
3
2
2
2
2
2
2
2
2
Application
Agonist (Agon), Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Western Blotting (WB)
778
211
185
160
143
140
133
129
89
77
26
18
13
9
8
6
3
3
3
3
3
2
2
2
1
1
1
1
1
1
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Available images

Catalog No. ABIN5012852
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5012853 Agon CyTOF FACS WB Mouse IgG1 AA 19-152 Log in to see 37407
1 ABIN5012855 Agon FACS ICC WB Goat IgG Log in to see Polyclonal

General

Antigen CD28 Antibodies
Epitope AA 19-152
(17), (16), (15), (12), (11), (11), (10), (10), (8), (7), (6), (4), (4), (4), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1)
Reactivity Human
(494), (410), (103), (70), (67), (42), (41), (34), (27), (23), (15), (7), (3), (3), (3), (2), (1), (1), (1)
Host Mouse
(526), (199), (92), (69), (40), (29), (12), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD28 antibody is un-conjugated
(112), (95), (74), (37), (21), (20), (19), (19), (18), (17), (17), (13), (13), (13), (13), (12), (12), (12), (8), (7), (6), (6), (6), (6), (6), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2)
Application Agonist (Agon), Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Western Blotting (WB)
(778), (211), (185), (160), (143), (140), (133), (129), (89), (77), (26), (18), (13), (9), (8), (6), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling Images
Specificity Detects human CD28 in Western blots. In Western blots, this antibody does not cross-react with recombinant mouse (rm) CD28, rhCTLA4, or rmCTLA4.
Purification Protein A or G purified from ascites
Endotoxin Level <0.10 EU per 1 μg of the antibody by the LAL method.
Immunogen S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD28, Asn19-Pro152, Accession # P10747
Clone 37407
Isotype IgG1

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background Alternative Names: CD28, CD28 antigen, CD28 antigen (Tp44), CD28 Molecule, MGC138290, T-cell-specific surface glycoprotein CD28, Tp44
Gene ID 940
UniProt P10747
Research Area Surface Receptors of Immune Cells, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Apoptosis/Necrosis, Immunology
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling Images back to top
Application Notes Western Blot 1 μg/mL
Flow Cytometry 0.25 μg/10^6 cells
Agonist Activity 0.2-0.6 μg/mL
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling back to top
Supplier Images
Functional Studies (Func) image for anti-CD28 (CD28) (AA 19-152) antibody (ABIN5012852) Human CD28 Antibody Enhances IL-2 Secretion in Jurkat Cells. Human CD28 Monoclonal An...
Functional Studies (Func) image for anti-CD28 (CD28) (AA 19-152) antibody (ABIN5012852) Human CD28 Antibody Enhances IL-2 Secretion in Jurkat Cells. Human CD28 Monoclonal An...