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anti-Human CD28 antibody for Functional Studies

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • CD28RNA
  • Tp44
Reactivity
Human
444
374
106
72
44
34
26
26
23
23
12
6
2
1
1
1
1
Host
Rat
516
203
77
67
28
25
12
Clonality
Monoclonal
Conjugate
This CD28 antibody is un-conjugated
118
99
78
41
17
17
15
13
13
12
11
10
9
9
9
9
9
9
9
8
8
8
6
5
4
4
3
2
2
2
2
2
2
2
2
2
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
748
275
182
132
123
118
116
114
89
66
22
13
8
4
3
3
3
3
3
2
2
1
1
1
1
1
1
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Catalog No. ABIN118582
$ 632.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008771965 ABIN638435 Func ICC IHC (fro) FACS IP WB Mouse IgG1 Log in to see CD28-2 7
0.0008771965 ABIN1106181 Func IHC (fro) FACS Mouse IgG2a Log in to see B-T3 4
0.0008771965 ABIN775705 Func ICC IHC (fro) FACS IP WB Mouse IgG1 Log in to see CD28-2 5
0.0008771965 ABIN400890 Func FACS IHC (fro) IF IP WB Mouse IgG1 Log in to see CD28-2
0.0008771965 ABIN781619 Func IHC (fro) FACS IF IP WB Mouse IgG1 Log in to see CD28-2
0.0008771965 ABIN610015 Func FACS ELISA PE Mouse IgG1, kappa Log in to see ANC28-1-5D10
0.0008771965 ABIN610014 Func FACS ELISA FITC Mouse IgG1, kappa Log in to see ANC28-1-5D10
0.0008771965 ABIN610013 Func FACS ELISA Biotin Mouse IgG1, kappa Log in to see ANC28-1-5D10
0.0008771965 ABIN282178 Func FACS Mouse IgG1 kappa Log in to see CD28-2
0.0008771965 ABIN217792 Func FACS IHC (fro) Rat IgG2b Log in to see
0.0008771965 ABIN280307 Func FACS IP IHC Mouse IgG1 kappa Log in to see CD28-2
0.0008771965 ABIN610012 Func FACS ELISA Mouse IgG1, kappa Log in to see ANC28-1-5D10
0.0008771965 ABIN1959966 Func ICC IHC (fro) FACS IP WB Mouse IgG1 Log in to see CD28-2
0.0008771965 ABIN610017 Func FACS ELISA Mouse IgG1, kappa Log in to see ANC28-1-5D10
0.0008771965 ABIN610016 Func FACS ELISA Mouse IgG1, kappa Log in to see ANC28-1-5D10
0.0008771965 ABIN200481 Func FACS Mouse IgG1 Log in to see
0.0008771965 ABIN1967135 Func ICC IHC (fro) FACS IP WB Mouse IgG1 Log in to see CD28-2
0.0008771965 ABIN600763 Func IHC (fro) FACS Rat IgG2b Log in to see
0.0008771965 ABIN2479702 Func IHC (fro) FACS Rat IgG2b Log in to see YTH913-12 3
0.0008771965 ABIN3200891 FACS Func ICC IHC (fro) IP WB APC Mouse IgG1 Log in to see CD28-2

Top referenced anti-CD28 antibody for Functional Studies

Similar anti-CD28 Antibodies

Application / Reactivity Human
Activation (Act) 1 Antibodies
Biochemical Assay (BCA) 3 Antibodies
ELISA 102 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 323 Antibodies
Functional Studies (Func) 68 Antibodies
Immunocytochemistry (ICC) 106 Antibodies
Immunofluorescence (IF) 118 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 47 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 139 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 50 Antibodies
Immunoprecipitation (IP) 115 Antibodies
Intracellular Flow Cytometry (ICFC) 1 Antibodies
Mass Cytometry (CyTOF) 2 Antibodies
Western Blotting (WB) 113 Antibodies

General

Antigen CD28 Antibodies
Reactivity Human
(444), (374), (106), (72), (44), (34), (26), (26), (23), (23), (12), (6), (2), (1), (1), (1), (1)
Host Rat
(516), (203), (77), (67), (28), (25), (12)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD28 antibody is un-conjugated
(118), (99), (78), (41), (17), (17), (15), (13), (13), (12), (11), (10), (9), (9), (9), (9), (9), (9), (9), (8), (8), (8), (6), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
(748), (275), (182), (132), (123), (118), (116), (114), (89), (66), (22), (13), (8), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling References for anti-CD28 Antibody (ABIN118582) Images
Specificity This antibody recognises the CD28 cell surface antigen, a 44 kDa glycoprotein expressed by T lymphocytes. The antibody enhances the MLR response. It has been reported that this clone also recognises an epitope of CD28 expressed by NK cells, which is not recognised by clones of 9.3 or CD28.2.
Purification Affinity chromatography on Protein G
Endotoxin Level Low Endotoxin
Immunogen Human peripheral blood T-cells. Spleen cells from an immunised DA rat were fused with cells of the Y3/Ag 1.2.3 rat myeloma cell line.
Clone YTH913-12
Isotype IgG2b

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling References for anti-CD28 Antibody (ABIN118582) Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background Human CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. It is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes (approximately 95 % of CD4+ cells and 50 % of CD8+ cells). Mature thymocytes exhibit higher levels of CD28 than the immature cells. Activation of T cells results in enhanced CD28 expression. T cell activation requires two combined signals provided by antigen presenting cells. The first is mediated via the T cell receptor following its interaction with antigenic peptide MHC complexes, and the second is delivered by accessory or costimulating molecules through their counter receptors on T lymphocytes. CD28 bears structural homology to CTLA 4 which is expressed at very low levels on the surface of CD4+ and CD8+ peripheral blood cells only following activation. CD28 is the natural receptor for the B7/BB 1 ligand (CD80) a 55-60 kDa glycoprotein which is expressed on activated B lymphocytes, on dendritic cells and on interferon gamma treated monocytes. The binding of B7 1/BB 1 Molecules to CD28 is involved in T lymphocyte activation and in the initiation and maintenance of chronic inflammation.Synonyms: T-cell-specific surface glycoprotein CD28, TP44
Gene ID 940
NCBI Accession NP_006130
UniProt P10747
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling References for anti-CD28 Antibody (ABIN118582) Images back to top
Application Notes Flow Cytometry: 1/50 - 1/100, Use 10 mLof the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections. Functional Assays: Stimulation of MLR response.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01 EU/ug
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details References for anti-CD28 Antibody (ABIN118582) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C

References for anti-CD28 Antibody (ABIN118582)

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling Images back to top
Background publications

Galea-Lauri, Darling, Gan, Krivochtchapov, Kuiper, Gäken, Souberbielle, Farzaneh: "Expression of a variant of CD28 on a subpopulation of human NK cells: implications for B7-mediated stimulation of NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 1, pp. 62-70, 1999

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling References for anti-CD28 Antibody (ABIN118582) back to top
Supplier Images
 image for anti-CD28 antibody (CD28) (ABIN118582) Staining of human peripheral blood lymphocytes with RAT ANTI HUMAN CD28