anti-Human CD28 antibody for Functional Studies

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • Tp44
  • CD28RNA
  • CD28 molecule
  • CD28 antigen
  • Cd28 molecule
  • CD28
  • Cd28
Reactivity
Human
478
390
103
70
51
34
27
26
25
22
15
7
3
3
3
2
1
1
1
Host
Human
511
194
91
70
40
29
12
Clonality
Monoclonal
Conjugate
This CD28 antibody is un-conjugated
111
94
72
37
20
19
18
18
17
16
16
12
12
12
12
11
10
10
8
7
6
6
6
6
6
5
4
3
3
2
2
2
2
2
2
2
2
Application
Flow Cytometry (FACS), Functional Studies (Func)
760
211
185
148
143
140
129
127
88
72
26
13
13
9
8
6
4
3
3
3
3
3
2
2
2
1
1
1
1
1
1
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Catalog No. ABIN5506227
$ 138.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN118582 Func FACS IHC (fro) Rat IgG2b Log in to see YTH913-12 1
1 ABIN1106181 Func IHC (fro) FACS Mouse IgG2a Log in to see B-T3 4
1 ABIN638435 FACS Func ICC IHC (f) IP WB Mouse IgG1 Log in to see CD28-2 7
1 ABIN775705 FACS Func ICC IHC (f) IP WB Mouse IgG1 Log in to see CD28-2 5
1 ABIN457388 FACS Func ICC IHC (f) IP WB PerCP Mouse IgG1 Log in to see CD28-2 6
1 ABIN349705 FACS Func ICC IHC (f) IP WB APC Mouse IgG1 Log in to see CD28-2 6
1 ABIN349707 FACS Func ICC IHC (f) IP WB FITC Mouse IgG1 Log in to see CD28-2 4
1 ABIN349710 FACS Func ICC IHC (f) IP WB PE Mouse IgG1 Log in to see CD28-2 6
1 ABIN400890 Func FACS IHC (fro) IF IP WB Mouse IgG1 Log in to see CD28-2
1 ABIN781619 Func IHC (fro) FACS IF IP WB Mouse IgG1 Log in to see CD28-2
1 ABIN610014 Func FACS ELISA FITC Mouse IgG1, kappa Log in to see ANC28-1-5D10
1 ABIN610013 Func FACS ELISA Biotin Mouse IgG1, kappa Log in to see ANC28-1-5D10
1 ABIN610015 Func FACS ELISA PE Mouse IgG1, kappa Log in to see ANC28-1-5D10
1 ABIN2618450 FACS Func ICC IHC (fro) IHC (p) IP Syrian Hamster IgG Log in to see 37-51
1 ABIN610012 Func FACS ELISA Mouse IgG1, kappa Log in to see ANC28-1-5D10
1 ABIN1959966 Func ICC IHC (fro) FACS IP WB Mouse IgG1 Log in to see CD28-2
1 ABIN2479702 Func IHC (fro) FACS Rat IgG2b Log in to see YTH913-12 3
1 ABIN610017 Func FACS ELISA Mouse IgG1, kappa Log in to see ANC28-1-5D10
1 ABIN610016 Func FACS ELISA Mouse IgG1, kappa Log in to see ANC28-1-5D10
1 ABIN1967135 Func ICC IHC (fro) FACS IP WB Mouse IgG1 Log in to see CD28-2

Top referenced anti-CD28 antibody for Functional Studies

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Application / Reactivity Human
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Agonist (Agon) 3 Antibodies
Biochemical Assay (BCA) 3 Antibodies
Cell Culture (CC) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 10 Antibodies
ELISA 123 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 352 Antibodies
Functional Studies (Func) 48 Antibodies
Immunocytochemistry (ICC) 108 Antibodies
Immunofluorescence (IF) 130 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 62 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 6 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 128 Antibodies

General

Antigen CD28 Antibodies
Reactivity Human
(478), (390), (103), (70), (51), (34), (27), (26), (25), (22), (15), (7), (3), (3), (3), (2), (1), (1), (1)
Host Human
(511), (194), (91), (70), (40), (29), (12)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD28 antibody is un-conjugated
(111), (94), (72), (37), (20), (19), (18), (18), (17), (16), (16), (12), (12), (12), (12), (11), (10), (10), (8), (7), (6), (6), (6), (6), (6), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2)
Application Flow Cytometry (FACS), Functional Studies (Func)
(760), (211), (185), (148), (143), (140), (129), (127), (88), (72), (26), (13), (13), (9), (8), (6), (4), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling Images
Specificity Human CD28
Endotoxin Level < 0.5 EU/mg
Clone H001
Isotype IgG2

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Research Area Adaptive Immunity, Immunology, Hematopoietic Progenitors, Surface Receptors of Immune Cells, CD Antigens, Apoptosis/Necrosis
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling Images back to top
Application Notes Agonist Activity - Human CD28 antibody induce IL2 secretion of the CD4+ T cells. The activating titer (EC50) is typically 0.15-0.6 μg/mL in the presence of 2 μg/mL Human CD3 (humanized OKT3) antibody(10977-H001) coated on the cell culture plate.
FCM: 0.5-2 μg/Test
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details Images back to top
Format Liquid
Buffer 0.2 μm filtered solution in Histidine and Arginine buffer containing 120 mM NaCl, 0.02 % Tween 80, pH 6.0
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Expiry Date 12 months

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD28 antibody (CD28) (ABIN5506227) The staining effect of Human CD28 antibody to CD28 of Human Peripheral Blood Lymphocy...
Functional Studies (Func) image for anti-CD28 antibody (CD28) (ABIN5506227) Effect of human CD28 antibody on stimulating IL-2 secretion of CD4 T cells co-activat...
Flow Cytometry (FACS) image for anti-CD28 antibody (CD28) (ABIN5506227) CD28+ T cell staining of lymphocytes using human CD28 antibody by Flow cytometric ana...