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anti-Human CD28 antibody for Western Blotting

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • CD28RNA
  • Tp44
Epitope
AA 182-208, C-Term
20
16
15
14
11
11
10
5
3
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
314
306
102
38
26
12
6
5
5
2
2
2
1
1
1
1
Host
Rabbit
360
189
76
42
25
20
7
Clonality
Polyclonal
Conjugate
This CD28 antibody is un-conjugated
106
90
66
41
10
8
7
7
7
6
5
5
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
541
174
113
95
92
43
41
33
27
22
13
8
4
3
3
3
3
3
2
2
1
1
1
1
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Catalog No. ABIN390970
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12303223 ABIN2787933 WB Rabbit C-Term Log in to see Polyclonal
0.12303223 ABIN615960 EIA WB Goat Internal Region, Extracellular Domain Log in to see Polyclonal
0.12303223 ABIN1531543 ELISA WB Rabbit IgG pTyr218 Log in to see Polyclonal
0.12303223 ABIN2464509 ELISA WB Goat Internal Region Log in to see Polyclonal
0.12303223 ABIN2704607 WB Rabbit C-Term, pTyr218 Log in to see Polyclonal
0.12303223 ABIN1714990 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN3187775 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
0.12303223 ABIN929126 WB Rabbit C-Term Log in to see Polyclonal
0.12303223 ABIN514171 WB Mouse AA 1-220, full length Log in to see Polyclonal
0.12303223 ABIN2158125 ELISA WB Rabbit Ig Fraction Log in to see Polyclonal
0.12303223 ABIN1575399 ELISA WB Rabbit IgG pTyr218 Log in to see Polyclonal
0.12303223 ABIN2155483 WB Goat Log in to see Polyclonal
0.0011806369 ABIN638435 Func ICC IHC (fro) FACS IP WB Mouse IgG1 Log in to see CD28-2 7
0.0011806369 ABIN674604 IF (p) IHC (p) WB Rabbit IgG AA 20-45 Log in to see Polyclonal 1
0.0011806369 ABIN775705 Func ICC IHC (fro) FACS IP WB Mouse IgG1 Log in to see CD28-2 5
0.0011806369 ABIN2658047 FACS IP WB Alexa Fluor 700 Mouse IgG1 kappa Log in to see CD28-2 9
0.0011806369 ABIN400890 Func FACS IHC (fro) IF IP WB Mouse IgG1 Log in to see CD28-2
0.0011806369 ABIN781619 Func IHC (fro) FACS IF IP WB Mouse IgG1 Log in to see CD28-2
0.0011806369 ABIN793286 ELISA WB Goat Internal Region Log in to see Polyclonal
0.0011806369 ABIN575768 WB Rabbit C-Term Log in to see Polyclonal

Top referenced anti-CD28 antibody for Western Blotting

Similar anti-CD28 Antibodies

Application / Reactivity Human
Activation (Act) 1 Antibodies
Biochemical Assay (BCA) 3 Antibodies
ELISA 79 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 188 Antibodies
Functional Studies (Func) 44 Antibodies
Immunocytochemistry (ICC) 22 Antibodies
Immunofluorescence (IF) 30 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 29 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 35 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 6 Antibodies
Immunoprecipitation (IP) 37 Antibodies
Intracellular Flow Cytometry (ICFC) 1 Antibodies
Mass Cytometry (CyTOF) 2 Antibodies
Western Blotting (WB) 95 Antibodies

General

Antigen CD28 Antibodies
Epitope AA 182-208, C-Term
(20), (16), (15), (14), (11), (11), (10), (5), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(314), (306), (102), (38), (26), (12), (6), (5), (5), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(360), (189), (76), (42), (25), (20), (7)
Clonality (Clone)
Polyclonal   ( )
Conjugate This CD28 antibody is un-conjugated
(106), (90), (66), (41), (10), (8), (7), (7), (7), (6), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Immunofluorescence (IF), Western Blotting (WB)
(541), (174), (113), (95), (92), (43), (41), (33), (27), (22), (13), (8), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling References for anti-CD28 Antibody (ABIN390970) Images
Specificity This CD28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188~217 amino acids from the C-terminal region of human CD28.
Predicted Reactivity Rabbit
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This CD28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 182-208 AA from the C-terminal region of human CD28.
Clone RB21742
Isotype Ig

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling References for anti-CD28 Antibody (ABIN390970) Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background CD28 costimulation is essential for CD4-positive T-cell proliferation, survival, interleukin-2 production, and T-helper type-2 (Th2) development.
Synonyms: T-cell-specific surface glycoprotein CD28, TP44, CD_antigen=CD28, Flags: Precursor, CD28
Pathways: Measles, T cell receptor signaling pathway, Rheumatoid arthritis, Intestinal immune network for IgA production, Viral myocarditis
Molecular Weight 25066 Da, Isoform 3 15,369Da
Gene ID 940
UniProt P10747
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling References for anti-CD28 Antibody (ABIN390970) Images back to top
Application Notes WB = 1:1000, IF = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details References for anti-CD28 Antibody (ABIN390970) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-CD28 Antibody (ABIN390970)

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling Images back to top
Background publications

Zocchi, Poggi, Crosti, Tongiani, Rugarli: "Signalling in human tumour infiltrating lymphocytes: the CD28 molecule is functional and is physically associated with the CD45R0 molecule." in: European journal of cancer (Oxford, England : 1990), Vol. 28A, Issue 4-5, pp. 749-54, 1992

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling References for anti-CD28 Antibody (ABIN390970) back to top
Supplier Images
Western Blotting (WB) image for anti-CD28 antibody (CD28) (AA 182-208) (ABIN390970) Western blot analysis of CD28 Antibody (C-term) (ABIN390970) in MDA-MB231 cell line l...
Immunofluorescence (IF) image for anti-CD28 antibody (CD28) (AA 182-208) (ABIN390970) Confocal immunofluorescent analysis of CD28 Antibody (C-term) (ABIN390970) with Hela ...