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anti-Human CD28 antibody for Western Blotting

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • Tp44
  • CD28RNA
  • CD28 molecule
  • CD28 antigen
  • Cd28 molecule
  • CD28
  • Cd28
Epitope
AA 182-208, C-Term
16
14
14
11
11
11
10
10
8
6
4
4
3
3
3
3
3
1
1
1
1
1
1
1
Reactivity
Human
468
410
111
72
50
34
27
27
25
24
15
6
2
2
2
2
1
1
1
Host
Rabbit
523
214
85
72
40
28
12
Clonality
Polyclonal
Conjugate
This CD28 antibody is un-conjugated
120
99
78
38
21
20
17
16
16
16
14
12
12
12
12
11
10
10
10
7
6
6
5
4
4
4
4
3
2
2
2
2
2
2
2
2
2
Application
Immunofluorescence (IF), Western Blotting (WB)
784
215
185
144
140
136
129
127
91
71
26
13
13
9
8
6
4
4
3
3
3
3
3
2
2
1
1
1
1
1
1
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Catalog No. ABIN390970
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0877867 ABIN2787933 WB Rabbit C-Term Log in to see Polyclonal
3.0877867 ABIN2464509 ELISA WB Goat Internal Region Log in to see Polyclonal
3.0877867 ABIN1531543 ELISA WB Rabbit IgG pTyr218 Log in to see Polyclonal
3.0877867 ABIN615960 EIA WB Goat Internal Region, Extracellular Domain Log in to see Polyclonal
3.0877867 ABIN3187775 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
3.0877867 ABIN2704607 WB Rabbit C-Term, pTyr218 Log in to see Polyclonal
3.0877867 ABIN408762 WB Rabbit AA 72-85 Log in to see Polyclonal
3.0877867 ABIN1714990 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal
3.0877867 ABIN3028547 IF ELISA WB Rabbit Ig Fraction AA 182-208 Log in to see Polyclonal
3.0877867 ABIN2561704 ELISA WB Goat IgG Internal Region Log in to see Polyclonal
3.0877867 ABIN2158125 ELISA WB Rabbit Ig Fraction Log in to see Polyclonal
3.0877867 ABIN514171 WB Mouse AA 1-220, full length Log in to see Polyclonal
3.0877867 ABIN929126 WB Rabbit C-Term Log in to see Polyclonal
3.0877867 ABIN1575399 ELISA WB Rabbit IgG pTyr218 Log in to see Polyclonal
3.0877867 ABIN2155483 WB Goat Log in to see Polyclonal
1 ABIN638435 FACS Func ICC IHC (f) IP WB Mouse IgG1 Log in to see CD28-2 7
1 ABIN775705 FACS Func ICC IHC (f) IP WB Mouse IgG1 Log in to see CD28-2 5
1 ABIN2658047 FACS IP WB Alexa Fluor 700 Mouse IgG1 kappa Log in to see CD28-2 9
1 ABIN349710 FACS Func ICC IHC (f) IP WB PE Mouse IgG1 Log in to see CD28-2 6
1 ABIN349705 FACS Func ICC IHC (f) IP WB APC Mouse IgG1 Log in to see CD28-2 6

Top referenced anti-CD28 antibody for Western Blotting

Similar anti-CD28 Antibodies

Application / Reactivity Human
Activation (Act) 1 Antibodies
Agonist (Agon) 3 Antibodies
Biochemical Assay (BCA) 3 Antibodies
Cytometry by Time of Flight (CyTOF) 10 Antibodies
ELISA 124 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 338 Antibodies
Functional Studies (Func) 47 Antibodies
Immunocytochemistry (ICC) 108 Antibodies
Immunofluorescence (IF) 127 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 62 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 6 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 127 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 48 Antibodies
Immunoprecipitation (IP) 53 Antibodies

General

Antigen CD28 Antibodies
Epitope AA 182-208, C-Term
(16), (14), (14), (11), (11), (11), (10), (10), (8), (6), (4), (4), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(468), (410), (111), (72), (50), (34), (27), (27), (25), (24), (15), (6), (2), (2), (2), (2), (1), (1), (1)
Host Rabbit
(523), (214), (85), (72), (40), (28), (12)
Clonality (Clone)
Polyclonal   ( )
Conjugate This CD28 antibody is un-conjugated
(120), (99), (78), (38), (21), (20), (17), (16), (16), (16), (14), (12), (12), (12), (12), (11), (10), (10), (10), (7), (6), (6), (5), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunofluorescence (IF), Western Blotting (WB)
(784), (215), (185), (144), (140), (136), (129), (127), (91), (71), (26), (13), (13), (9), (8), (6), (4), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling References for anti-CD28 Antibody (ABIN390970) Images
Specificity This CD28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188~217 amino acids from the C-terminal region of human CD28.
Predicted Reactivity Rabbit
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This CD28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 182-208 AA from the C-terminal region of human CD28.
Clone RB21742
Isotype Ig

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling References for anti-CD28 Antibody (ABIN390970) Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background CD28 costimulation is essential for CD4-positive T-cell proliferation, survival, interleukin-2 production, and T-helper type-2 (Th2) development.
Synonyms: T-cell-specific surface glycoprotein CD28, TP44, CD_antigen=CD28, Flags: Precursor, CD28
Pathways: Measles, T cell receptor signaling pathway, Rheumatoid arthritis, Intestinal immune network for IgA production, Viral myocarditis
Molecular Weight 25066 Da, Isoform 3 15,369Da
Gene ID 940
UniProt P10747
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling References for anti-CD28 Antibody (ABIN390970) Images back to top
Application Notes WB = 1:1000, IF = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details References for anti-CD28 Antibody (ABIN390970) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-CD28 Antibody (ABIN390970)

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling Images back to top
Background publications

Zocchi, Poggi, Crosti, Tongiani, Rugarli: "Signalling in human tumour infiltrating lymphocytes: the CD28 molecule is functional and is physically associated with the CD45R0 molecule." in: European journal of cancer (Oxford, England : 1990), Vol. 28A, Issue 4-5, pp. 749-54, 1992

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling References for anti-CD28 Antibody (ABIN390970) back to top
Supplier Images
Western Blotting (WB) image for anti-CD28 antibody (CD28) (AA 182-208) (ABIN390970) Western blot analysis of CD28 Antibody (C-term) (ABIN390970) in MDA-MB231 cell line l...
Immunofluorescence (IF) image for anti-CD28 antibody (CD28) (AA 182-208) (ABIN390970) Confocal immunofluorescent analysis of CD28 Antibody (C-term) (ABIN390970) with Hela ...