anti-Human CD28 antibody for Western Blotting

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • Tp44
  • CD28RNA
  • CD28 molecule
  • CD28 antigen
  • Cd28 molecule
  • CD28
  • Cd28
Epitope
C-Term
16
16
15
12
11
11
11
10
8
7
6
4
4
4
3
3
3
3
3
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
495
409
102
70
67
41
40
34
27
22
15
7
2
2
2
2
1
1
Host
Rabbit
527
199
91
70
40
29
12
1
Clonality
Polyclonal
Conjugate
This CD28 antibody is un-conjugated
112
96
74
37
21
20
19
19
18
17
17
13
13
13
13
12
12
12
8
7
6
6
6
6
6
5
4
3
3
2
2
2
2
2
2
2
2
Application
Western Blotting (WB)
780
211
185
160
143
140
133
129
89
77
26
19
13
9
8
6
4
3
3
3
3
2
2
2
1
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN2787933
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.9476938 ABIN390970 IF WB Rabbit Ig AA 182-208, C-Term Log in to see Polyclonal 1
2.9476938 ABIN2704607 WB Rabbit C-Term, pTyr218 Log in to see Polyclonal
2.9476938 ABIN2464509 ELISA WB Goat Internal Region Log in to see Polyclonal
2.9476938 ABIN1531543 ELISA WB Rabbit IgG pTyr218 Log in to see Polyclonal
2.9476938 ABIN3187775 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
2.9476938 ABIN408762 WB Rabbit AA 72-85 Log in to see Polyclonal
2.9476938 ABIN1714990 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal
2.9476938 ABIN3028547 IF ELISA WB Rabbit Ig Fraction AA 182-208 Log in to see Polyclonal
2.9476938 ABIN514171 WB Mouse AA 1-220, full length Log in to see Polyclonal
2.9476938 ABIN2561704 ELISA WB Goat IgG Internal Region Log in to see Polyclonal
2.9476938 ABIN929126 WB Rabbit C-Term Log in to see Polyclonal
2.9476938 ABIN1575399 ELISA WB Rabbit IgG pTyr218 Log in to see Polyclonal
1 ABIN638435 FACS Func ICC IHC (f) IP WB Mouse IgG1 Log in to see CD28-2 7
1 ABIN775705 FACS Func ICC IHC (f) IP WB Mouse IgG1 Log in to see CD28-2 5
1 ABIN793286 ELISA WB Goat AA 73-87 Log in to see Polyclonal
1 ABIN349705 FACS Func ICC IHC (f) IP WB APC Mouse IgG1 Log in to see CD28-2 6
1 ABIN349707 FACS Func ICC IHC (f) IP WB FITC Mouse IgG1 Log in to see CD28-2 4
1 ABIN349710 FACS Func ICC IHC (f) IP WB PE Mouse IgG1 Log in to see CD28-2 6
1 ABIN457388 FACS Func ICC IHC (f) IP WB PerCP Mouse IgG1 Log in to see CD28-2 6
1 ABIN575768 WB Rabbit C-Term Log in to see Polyclonal

General

Antigen CD28 Antibodies
Epitope C-Term
(16), (16), (15), (12), (11), (11), (11), (10), (8), (7), (6), (4), (4), (4), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
(495), (409), (102), (70), (67), (41), (40), (34), (27), (22), (15), (7), (2), (2), (2), (2), (1), (1)
Host Rabbit
(527), (199), (91), (70), (40), (29), (12), (1)
Clonality
Polyclonal
Conjugate This CD28 antibody is un-conjugated
(112), (96), (74), (37), (21), (20), (19), (19), (18), (17), (17), (13), (13), (13), (13), (12), (12), (12), (8), (7), (6), (6), (6), (6), (6), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2)
Application Western Blotting (WB)
(780), (211), (185), (160), (143), (140), (133), (129), (89), (77), (26), (19), (13), (9), (8), (6), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Supplier Log in to see

Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling Images
Blocking Peptide Blocking peptide for this product available: ABIN974664
Sequence TVAFIIFWVR SKRSRLLHSD YMNMTPRRPG PTRKHYQPYA PPRDFAAYRS
Predicted Reactivity Cow: 93%, Dog: 93%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 86%, Pig: 100%, Rabbit: 100%, Rat: 93%, Sheep: 93%
Characteristics This is a rabbit polyclonal antibody against CD28. It was validated on Western Blot.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human CD28

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background CD28 is involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.
Alias Symbols: MGC138290, Tp44
Protein Interaction Partner: VAV1, DOK1, LRRC23, CD80, UBC, PIK3R2, PIK3R1, PIK3CA, DUSP14, GRAP2, NSF, PIK3CG, CD86, ITK, CD4, CD247, PLCG1, IL12A, LCK, PTPRC, GRB2,
Protein Size: 220
Molecular Weight 25 kDa
Gene ID 940
NCBI Accession NM_006139, NP_006130
UniProt P10747
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 220 AA

Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-CD28 (CD28) (C-Term) antibody (ABIN2787933) WB Suggested Anti-CD28 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...
Western Blotting (WB) image for anti-CD28 (CD28) (C-Term) antibody (ABIN2787933) WB Suggested Anti-CD28
Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:.12500 <...