You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Mouse (Murine) CD28 antibody for Blocking Reagent

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • CD28RNA
  • Tp44
Reactivity
Mouse (Murine)
445
373
106
72
44
34
26
26
23
23
12
6
2
1
1
1
1
Host
Syrian Hamster
516
203
77
68
27
25
12
Clonality
Monoclonal
Conjugate
This CD28 antibody is un-conjugated
118
99
78
41
17
17
15
13
13
12
11
10
9
9
9
9
9
9
9
8
8
8
6
5
4
4
3
2
2
2
2
2
2
2
2
2
1
1
1
Application
Blocking Reagent (BR), Cytotoxicity Test (CyTox), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP)
748
274
182
132
123
118
117
114
89
66
22
13
8
4
3
3
3
2
2
2
2
1
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Catalog No. ABIN1176978
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008709465 ABIN2689144 BR CyTox IHC (fro) FACS IP Syrian Hamster IgG2, lambda Log in to see 37-51 13
0.0008709465 ABIN2689145 BR CyTox IHC (fro) FACS IP Syrian Hamster IgG2, lambda Log in to see 37-51

Top referenced anti-CD28 antibody for Blocking Reagent

Similar anti-CD28 Antibodies

Application / Reactivity Mouse (Murine)
Activation (Act) 2 Antibodies
Blocking Antibody (Inhibition) 1 Antibodies
Blocking Reagent (BR) 3 Antibodies
Cytotoxicity Test (CyTox) 3 Antibodies
ELISA 22 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 1 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 288 Antibodies
Functional Studies (Func) 27 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 30 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 15 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 22 Antibodies
Immunoprecipitation (IP) 91 Antibodies
In vitro Assay (in vitro) 7 Antibodies
Intracellular Flow Cytometry (ICFC) 9 Antibodies

General

Antigen CD28 Antibodies
Reactivity Mouse (Murine)
(445), (373), (106), (72), (44), (34), (26), (26), (23), (23), (12), (6), (2), (1), (1), (1), (1)
Host Syrian Hamster
(516), (203), (77), (68), (27), (25), (12)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD28 antibody is un-conjugated
(118), (99), (78), (41), (17), (17), (15), (13), (13), (12), (11), (10), (9), (9), (9), (9), (9), (9), (9), (8), (8), (8), (6), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Blocking Reagent (BR), Cytotoxicity Test (CyTox), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP)
(748), (274), (182), (132), (123), (118), (117), (114), (89), (66), (22), (13), (8), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 14 references available
Supplier Log in to see

Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling References for anti-CD28 Antibody (ABIN1176978) Images
Brand BD Pharmingen™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
Immunogen Mouse EL-4 (T-cell lymphoma) Cells
Clone 37-51
Isotype IgG2, lambda

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling References for anti-CD28 Antibody (ABIN1176978) Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background The 37.51 antibody reacts with CD28, which is expressed on most thymocytes, at low density on nearly all CD4+ and CD8+ peripheral T cells, and at even lower density on NK cells. The expression of CD28, in splenocytes and thymocytes, has been reported to increase after activation. CD28 transcripts are found in mast cells, and cell-surface expression of CD28 is induced upon maturation or activation of mast cells. It has been reported that CD28 is not expressed on some populations of intraepithelial T lymphocytes. CD28 is a costimulatory receptor, its ligands include CD80 (B7-1) and CD86 (B7-2). The 37.51 mAb augments proliferation and cytokine production by activated T and NK cells and can provide a costimulatory signal for CTL induction. There is considerable evidence that CD28 is a costimulatory receptor involved in many, but not all, T cell-dependent immune responses.
Research Area Apoptosis/Necrosis
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling References for anti-CD28 Antibody (ABIN1176978) Images back to top
Application Notes Flow Cytometry: For multicolor staining of cell suspensions from peripheral lymphoid tissues (e.g detection of T lymphocytes and/or NK cells), investigators are encouraged to utilize Purified Rat Anti-Mouse CD16/CD32 [Mouse BD Fc Block™] (Cat. No. 553141) to minimize non-specific binding. In addition, since CD28 may be expressed at low density on resting peripheral T lymphocytes, investigators may wish to amplify the staining signal by using a biotinylated second-step antibody followed by a bright third-step reagent, such as PE Streptavidin (Cat. No. 554061). If Mouse BD Fc Block™ is used, investigators need to be aware of and account for any potential crossreactivity that may occur between the second-step or third-step reagents chosen with the Mouse BD Fc Block™. Biotin Mouse Anti-Syrian Hamster IgG2 (Cat. No. 554029) would be a suggested second-step antibody for investigators to consider utilizing.
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details References for anti-CD28 Antibody (ABIN1176978) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer No azide/low endotoxin: Aqueous buffered solution containing protein stabilizer, no preservative, 0.2μm sterile filtered.
Preservative Azide free
Storage 4 °C
Storage Comment Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-CD28 Antibody (ABIN1176978)

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling Images back to top
Product cited in:

Marietta, Weis, Weis: "CD28 expression by mouse mast cells is modulated by lipopolysaccharide and outer surface protein A lipoprotein from Borrelia burgdorferi." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 6, pp. 2840-8, 1997

Ong, Lim, Teh: "CD28-induced cytokine production and proliferation by thymocytes are differentially regulated by the p59fyn tyrosine kinase." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 5, pp. 2169-76, 1997

Cibotti, Punt, Dash, Sharrow, Singer: "Surface molecules that drive T cell development in vitro in the absence of thymic epithelium and in the absence of lineage-specific signals." in: Immunity, Vol. 6, Issue 3, pp. 245-55, 1997

Masten, Yates, Pollard Koga, Lipscomb: "Characterization of accessory molecules in murine lung dendritic cell function: roles for CD80, CD86, CD54, and CD40L." in: American journal of respiratory cell and molecular biology, Vol. 16, Issue 3, pp. 335-42, 1997

Rakasz, Hagen, Sandor, Lynch: "Gamma delta T cells of the murine vagina: T cell response in vivo in the absence of the expression of CD2 and CD28 molecules." in: International immunology, Vol. 9, Issue 1, pp. 161-7, 1997

Nishio, Spielman, Lee, Nelson, Podack: "CD80 (B7.1) and CD54 (intracellular adhesion molecule-1) induce target cell susceptibility to promiscuous cytotoxic T cell lysis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 10, pp. 4347-53, 1996

Gelfanov, Lai, Gelfanova, Dong, Su, Liao: "Differential requirement of CD28 costimulation for activation of murine CD8+ intestinal intraepithelial lymphocyte subsets and lymph node cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 1, pp. 76-82, 1995

Bluestone: "New perspectives of CD28-B7-mediated T cell costimulation." in: Immunity, Vol. 2, Issue 6, pp. 555-9, 1995

June, Bluestone, Nadler, Thompson: "The B7 and CD28 receptor families." in: Immunology today, Vol. 15, Issue 7, pp. 321-31, 1994

Nandi, Gross, Allison: "CD28-mediated costimulation is necessary for optimal proliferation of murine NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 7, pp. 3361-9, 1994

Harding, Allison: "CD28-B7 interactions allow the induction of CD8+ cytotoxic T lymphocytes in the absence of exogenous help." in: The Journal of experimental medicine, Vol. 177, Issue 6, pp. 1791-6, 1993

Shahinian, Pfeffer, Lee, Kündig, Kishihara, Wakeham, Kawai, Ohashi, Thompson, Mak: "Differential T cell costimulatory requirements in CD28-deficient mice." in: Science (New York, N.Y.), Vol. 261, Issue 5121, pp. 609-12, 1993

Gross, Callas, Allison: "Identification and distribution of the costimulatory receptor CD28 in the mouse." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 2, pp. 380-8, 1992

Harding, McArthur, Gross, Raulet, Allison: "CD28-mediated signalling co-stimulates murine T cells and prevents induction of anergy in T-cell clones." in: Nature, Vol. 356, Issue 6370, pp. 607-9, 1992