anti-Mouse (Murine) CD28 antibody for Functional Studies

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • Tp44
  • CD28RNA
  • CD28 molecule
  • CD28 antigen
  • Cd28 molecule
  • CD28
  • Cd28
Epitope
Extracellular Domain
16
16
15
12
11
11
11
10
8
7
6
4
4
4
3
3
3
3
2
1
1
1
1
1
1
Reactivity
Mouse (Murine)
496
421
103
70
67
42
41
34
27
23
15
7
3
3
3
2
1
1
1
Host
Hamster
527
202
92
77
40
30
12
1
Clonality
Monoclonal
Conjugate
This CD28 antibody is un-conjugated
113
95
74
38
22
21
20
20
18
18
17
13
13
13
13
12
12
12
8
8
7
7
7
6
6
5
4
3
3
2
2
2
2
2
2
2
2
1
Application
Functional Studies (Func), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
794
210
184
160
143
142
133
129
88
77
26
21
13
9
8
6
6
3
3
3
3
2
2
2
1
1
1
1
1
1
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Catalog No. ABIN457400
$ 173.25
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN181985 Func FACS Mouse IgG2b Log in to see E18 5
1 ABIN2618450 FACS Func ICC IHC (fro) IHC (p) IP Syrian Hamster IgG Log in to see 37-51
1 ABIN2479686 Func FACS Mouse IgG2b Log in to see E18 4
1 ABIN370908 Func FACS IP Hamster IgG Log in to see PV-1 9
1 ABIN370909 Func FACS IP Hamster IgG Log in to see PV-1 8
1 ABIN370901 Func FACS IP Hamster IgG Log in to see 37-51 16
1 ABIN370900 Func FACS IP Hamster IgG Log in to see 37-51 7
1 ABIN440723 CyTOF FACS Func ICC IF IHC IHC (fro) IP Syrian Hamster IgG Log in to see 37-51
1 ABIN217765 Func FACS Mouse IgG2b Log in to see
1 ABIN3199703 FACS Func ICC IHC (fro) IP Syrian Hamster IgG Log in to see 37-51
1 ABIN3025652 Func FACS IF Mouse IgG2a, kappa Log in to see 204-12
1 ABIN2567435 Func FACS IP Hamster IgG Log in to see 37-51
1 ABIN2567438 Func FACS IP Hamster IgG Log in to see 37-51
1 ABIN2567436 Func FACS IP Hamster IgG Log in to see 37-51
1 ABIN2567434 Func FACS IP Hamster IgG Log in to see 37-51
1 ABIN2567437 Func FACS IP Hamster IgG Log in to see 37-51
1 ABIN2962182 Func ICC IHC (fro) FACS IHC (p) WB Syrian Hamster IgG Log in to see (37-51)
1 ABIN3025653 Func FACS IF Mouse IgG2a, kappa Log in to see 204-12
1 ABIN642445 Func FACS Mouse Log in to see

General

Antigen CD28 Antibodies
Epitope Extracellular Domain
(16), (16), (15), (12), (11), (11), (11), (10), (8), (7), (6), (4), (4), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(496), (421), (103), (70), (67), (42), (41), (34), (27), (23), (15), (7), (3), (3), (3), (2), (1), (1), (1)
Host Hamster
(527), (202), (92), (77), (40), (30), (12), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD28 antibody is un-conjugated
(113), (95), (74), (38), (22), (21), (20), (20), (18), (18), (17), (13), (13), (13), (13), (12), (12), (12), (8), (8), (7), (7), (7), (6), (6), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Functional Studies (Func), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
(794), (210), (184), (160), (143), (142), (133), (129), (88), (77), (26), (21), (13), (9), (8), (6), (6), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 10 references available
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Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling References for anti-CD28 antibody (ABIN457400) Images
Specificity The Syrian hamster monoclonal antibody 37.51 reacts with extracellular domain of mouse CD28 costimulatory receptor, a 45 kDa homodimeric protein expressed by thymocytes, mature T cells and NK cells.
Purification Purified from cell culture supernatant by protein-G affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
Immunogen Mouse T cell lymphoma EL-4 cells
Clone 37-51
Isotype IgG

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling References for anti-CD28 antibody (ABIN457400) Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells.
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling References for anti-CD28 antibody (ABIN457400) Images back to top
Application Notes Flow Cytometry: Recommended dilution: 6 µg/mL
Immunoprecipitation: Recommended dilution: 1-2 µg / 100-500 µg of protein in 1 mL lysate
Immunocytochemistry: Recommended dilution: 1-4 µg/mL
Functional Application: In vitro and in vivo T cell costimulation

Working concentrations should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details References for anti-CD28 antibody (ABIN457400) Images back to top
Concentration 1 mg/mL
Buffer Azide free phosphate buffered saline (PBS), approx. pH 7.4.
Preservative Azide free
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.

References for anti-CD28 antibody (ABIN457400)

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling Images back to top
Product cited in:

Rosenberger, Clark, Treuting, Johnson, Aderem: "ATF3 regulates MCMV infection in mice by modulating IFN-gamma expression in natural killer cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 7, pp. 2544-9, 2008

Zhang, Hopkins, He: "The long isoform of cellular FLIP is essential for T lymphocyte proliferation through an NF-kappaB-independent pathway." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 8, pp. 5506-11, 2008

Perchonock, Pajerowski, Nguyen, Shapiro, Shapiro: "The related adaptors, adaptor in lymphocytes of unknown function X and Rlk/Itk-binding protein, have nonredundant functions in lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 3, pp. 1768-75, 2007

Pua, Dzhagalov, Chuck, Mizushima, He: "A critical role for the autophagy gene Atg5 in T cell survival and proliferation." in: The Journal of experimental medicine, Vol. 204, Issue 1, pp. 25-31, 2007

Perchonock, Fernando, Quinn, Nguyen, Sun, Shapiro, Shapiro: "Negative regulation of interleukin-2 and p38 mitogen-activated protein kinase during T-cell activation by the adaptor ALX." in: Molecular and cellular biology, Vol. 26, Issue 16, pp. 6005-15, 2006

Albert, Yu, Martin, Anasetti: "Prevention of lethal acute GVHD with an agonistic CD28 antibody and rapamycin." in: Blood, Vol. 105, Issue 3, pp. 1355-61, 2005

Zhang, He: "An essential role for c-FLIP in the efficient development of mature T lymphocytes." in: The Journal of experimental medicine, Vol. 202, Issue 3, pp. 395-404, 2005

Zhang, He: "The antiapoptotic protein Bcl-xL is dispensable for the development of effector and memory T lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 11, pp. 6967-73, 2005

Gross, Callas, Allison: "Identification and distribution of the costimulatory receptor CD28 in the mouse." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 2, pp. 380-8, 1992

Harding, McArthur, Gross, Raulet, Allison: "CD28-mediated signalling co-stimulates murine T cells and prevents induction of anergy in T-cell clones." in: Nature, Vol. 356, Issue 6370, pp. 607-9, 1992