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|Application / Reactivity||Mouse (Murine)|
|Activation (Act)||2 Antibodies|
|Blocking Antibody (Inhibition)||1 Antibodies|
|Blocking Reagent (BR)||4 Antibodies|
|Cytotoxicity Test (CyTox)||3 Antibodies|
|ELISA (Capture)||2 Antibodies|
|ELISA (Detection)||1 Antibodies|
|ELISpot (Detection) (ELISPOT (Detection))||1 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies|
|Flow Cytometry (FACS)||238 Antibodies|
|Functional Studies (Func)||27 Antibodies|
|Immunocytochemistry (ICC)||6 Antibodies|
|Immunofluorescence (IF)||7 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||22 Antibodies|
|Immunohistochemistry (IHC)||15 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||9 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||8 Antibodies|
|Immunoprecipitation (IP)||91 Antibodies|
|In vitro Assay (in vitro)||7 Antibodies|
|Intracellular Flow Cytometry (ICFC)||9 Antibodies|
|Epitope||Extracellular Domain Alternatives|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This CD28 antibody is un-conjugated Alternatives|
Functional Studies (Func), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
|10 references available|
|Supplier||Log in to see|
Product Details anti-CD28 AntibodyTarget Details CD28 Application Details Handling References for anti-CD28 Antibody (ABIN457400) Images
|Specificity||The Syrian hamster monoclonal antibody 37.51 reacts with extracellular domain of mouse CD28 costimulatory receptor, a 45 kDa homodimeric protein expressed by thymocytes, mature T cells and NK cells.|
|Purification||Purified from cell culture supernatant by protein-G affinity chromatography.|
|Purity||> 95 % (by SDS-PAGE)|
|Sterility||0.2 μm filtered|
|Endotoxin Level||Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.|
|Immunogen||Mouse T cell lymphoma EL-4 cells|
Target Details CD28Product Details anti-CD28 Antibody Application Details Handling References for anti-CD28 Antibody (ABIN457400) Images back to top
|Alternative Name||CD28 (CD28 Antibody Abstract)|
|Background||CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells.|
|Research Area||Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis|
|Pathways||TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway|
Application DetailsProduct Details anti-CD28 Antibody Target Details CD28 Handling References for anti-CD28 Antibody (ABIN457400) Images back to top
Flow Cytometry: Recommended dilution: 6 µg/mL
Immunoprecipitation: Recommended dilution: 1-2 µg / 100-500 µg of protein in 1 mL lysate
Immunocytochemistry: Recommended dilution: 1-4 µg/mL
Functional Application: In vitro and in vivo T cell costimulation
Working concentrations should be determined by the investigator.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD28 Antibody Target Details CD28 Application Details References for anti-CD28 Antibody (ABIN457400) Images back to top
|Buffer||Azide free phosphate buffered saline (PBS), approx. pH 7.4.|
|Handling Advice||Do not freeze.|
|Storage Comment||Store at 2-8 °C. Do not use after expiration date stamped on vial label.|
References for anti-CD28 Antibody (ABIN457400)Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling Images back to top
|Product cited in:||
Zhang, Hopkins, He: "The long isoform of cellular FLIP is essential for T lymphocyte proliferation through an NF-kappaB-independent pathway." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 8, pp. 5506-11, 2008 (PubMed).
Rosenberger, Clark, Treuting et al.: "ATF3 regulates MCMV infection in mice by modulating IFN-gamma expression in natural killer cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 7, pp. 2544-9, 2008 (PubMed).
Perchonock, Pajerowski, Nguyen et al.: "The related adaptors, adaptor in lymphocytes of unknown function X and Rlk/Itk-binding protein, have nonredundant functions in lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 3, pp. 1768-75, 2007 (PubMed).
Pua, Dzhagalov, Chuck et al.: "A critical role for the autophagy gene Atg5 in T cell survival and proliferation." in: The Journal of experimental medicine, Vol. 204, Issue 1, pp. 25-31, 2007 (PubMed).
Perchonock, Fernando, Quinn et al.: "Negative regulation of interleukin-2 and p38 mitogen-activated protein kinase during T-cell activation by the adaptor ALX." in: Molecular and cellular biology, Vol. 26, Issue 16, pp. 6005-15, 2006 (PubMed).
Zhang, He: "An essential role for c-FLIP in the efficient development of mature T lymphocytes." in: The Journal of experimental medicine, Vol. 202, Issue 3, pp. 395-404, 2005 (PubMed).
Zhang, He: "The antiapoptotic protein Bcl-xL is dispensable for the development of effector and memory T lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 11, pp. 6967-73, 2005 (PubMed).
Albert, Yu, Martin et al.: "Prevention of lethal acute GVHD with an agonistic CD28 antibody and rapamycin." in: Blood, Vol. 105, Issue 3, pp. 1355-61, 2005 (PubMed).
Gross, Callas, Allison: "Identification and distribution of the costimulatory receptor CD28 in the mouse." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 2, pp. 380-8, 1992 (PubMed).
Harding, McArthur, Gross et al.: "CD28-mediated signalling co-stimulates murine T cells and prevents induction of anergy in T-cell clones." in: Nature, Vol. 356, Issue 6370, pp. 607-9, 1992 (PubMed).