anti-Mouse (Murine) CD28 antibody for Western Blotting

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • Tp44
  • CD28RNA
  • CD28 molecule
  • CD28 antigen
  • Cd28 molecule
  • CD28
  • Cd28
Epitope
C-Term
16
15
15
12
11
11
11
10
8
7
6
4
4
4
3
3
3
3
3
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
478
389
102
70
51
34
27
25
24
21
15
7
2
2
2
2
1
1
Host
Rabbit
511
194
90
70
40
29
12
1
Clonality
Polyclonal
Conjugate
This CD28 antibody is un-conjugated
111
94
72
37
20
19
18
18
17
16
16
12
12
12
12
11
10
10
8
7
6
6
6
6
6
5
4
3
3
2
2
2
2
2
2
2
2
Application
Western Blotting (WB)
761
211
185
148
143
139
129
127
89
72
26
13
13
9
8
6
4
3
3
3
3
3
2
2
2
1
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN2787933
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.3442712 ABIN1531543 ELISA WB Rabbit IgG pTyr218 Log in to see Polyclonal
3.3442712 ABIN1714990 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal
3.3442712 ABIN2464553 ELISA WB Goat Internal Region Log in to see Polyclonal
3.3442712 ABIN2704607 WB Rabbit C-Term, pTyr218 Log in to see Polyclonal
3.3442712 ABIN2118849 ICC IHC IP WB Rabbit IgG AA 33-174 Log in to see Polyclonal
3.3442712 ABIN2561705 ELISA WB Goat IgG Internal Region Log in to see Polyclonal
3.3442712 ABIN5506224 ELISA WB Rabbit IgG AA 1-149 Log in to see Polyclonal
3.3442712 ABIN1575399 ELISA WB Rabbit IgG pTyr218 Log in to see Polyclonal
1 ABIN793287 ELISA WB Goat AA 137-149, Extracellular Domain Log in to see Polyclonal
1 ABIN575768 WB Rabbit C-Term Log in to see Polyclonal
1 ABIN2665489 IF IP IHC WB Hamster IgG Log in to see 37-51 9
1 ABIN1584203 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN1712505 IHC (p) WB HRP Rabbit IgG Log in to see Polyclonal
1 ABIN3182245 ELISA WB Rabbit IgG pTyr218 Log in to see Polyclonal
1 ABIN1701488 IHC (p) WB Biotin Rabbit IgG Log in to see Polyclonal
1 ABIN1452525 ELISA WB Rabbit IgG pTyr218, C-Term Log in to see Polyclonal
1 ABIN2665488 IF IP IHC WB Hamster IgG Log in to see 37-51 9
1 ABIN2883595 ELISA WB Rabbit IgG pTyr218 Log in to see Polyclonal
1 ABIN5554113 EIA WB Goat Internal Region Log in to see Polyclonal
1 ABIN2592985 ELISA WB Rabbit IgG AA 33-174 Log in to see Polyclonal

Similar anti-CD28 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Guinea Pig Horse (Equine) Rabbit Cow (Bovine) Sheep (Ovine) Dog (Canine) Pig (Porcine)
Immunofluorescence (fixed cells) (IF/ICC) 7 Antibodies 2 Antibodies
Cytometry by Time of Flight (CyTOF) 4 Antibodies 10 Antibodies
Western Blotting (WB) 116 Antibodies 36 Antibodies 8 Antibodies 3 Antibodies 3 Antibodies 3 Antibodies 2 Antibodies 2 Antibodies 1 Antibodies 1 Antibodies
Staining Methods (StM) 1 Antibodies
Neutralization (Neut) 1 Antibodies
Mass Cytometry (CyTOF) 2 Antibodies 1 Antibodies
Intracellular Flow Cytometry (ICFC) 9 Antibodies

General

Antigen CD28 Antibodies
Epitope C-Term
(16), (15), (15), (12), (11), (11), (11), (10), (8), (7), (6), (4), (4), (4), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
(478), (389), (102), (70), (51), (34), (27), (25), (24), (21), (15), (7), (2), (2), (2), (2), (1), (1)
Host Rabbit
(511), (194), (90), (70), (40), (29), (12), (1)
Clonality
Polyclonal
Conjugate This CD28 antibody is un-conjugated
(111), (94), (72), (37), (20), (19), (18), (18), (17), (16), (16), (12), (12), (12), (12), (11), (10), (10), (8), (7), (6), (6), (6), (6), (6), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2)
Application Western Blotting (WB)
(761), (211), (185), (148), (143), (139), (129), (127), (89), (72), (26), (13), (13), (9), (8), (6), (4), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Supplier Log in to see

Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling Images
Blocking Peptide Blocking peptide for this product available: ABIN974664
Sequence TVAFIIFWVR SKRSRLLHSD YMNMTPRRPG PTRKHYQPYA PPRDFAAYRS
Predicted Reactivity Cow: 93%, Dog: 93%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 86%, Pig: 100%, Rabbit: 100%, Rat: 93%, Sheep: 93%
Characteristics This is a rabbit polyclonal antibody against CD28. It was validated on Western Blot.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human CD28

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background CD28 is involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.
Alias Symbols: MGC138290, Tp44
Protein Interaction Partner: VAV1, DOK1, LRRC23, CD80, UBC, PIK3R2, PIK3R1, PIK3CA, DUSP14, GRAP2, NSF, PIK3CG, CD86, ITK, CD4, CD247, PLCG1, IL12A, LCK, PTPRC, GRB2,
Protein Size: 220
Molecular Weight 25 kDa
Gene ID 940
NCBI Accession NM_006139, NP_006130
UniProt P10747
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 220 AA

Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-CD28 antibody (CD28) (C-Term) (ABIN2787933) WB Suggested Anti-CD28 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...
Western Blotting (WB) image for anti-CD28 antibody (CD28) (C-Term) (ABIN2787933) WB Suggested Anti-CD28
Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:.12500 <...