You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Non-Human Primate CD28 antibody for Immunocytochemistry

Recommended CD28 Antibody (supplied by: Log in to see )

Antigen
CD28 Antibodies
  • CD28RNA
  • Tp44
Reactivity
Human, Non-Human Primate
314
306
102
38
26
12
5
5
5
2
2
2
1
1
1
1
Host
Mouse
359
189
77
42
25
20
7
Clonality
Monoclonal
Conjugate
This CD28 antibody is un-conjugated
106
90
66
41
10
8
7
7
7
6
5
5
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Functional Studies (Func), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
540
173
113
95
91
42
41
34
26
22
13
8
4
3
3
3
3
3
2
2
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN638435
$ 231.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010090914 ABIN775705 Func ICC IHC (fro) FACS IP WB Mouse IgG1 Log in to see CD28-2 5

Top referenced anti-CD28 antibody for Immunocytochemistry

Similar anti-CD28 Antibodies

Application / Reactivity Non-Human Primate Human
Flow Cytometry (FACS) 6 Antibodies 188 Antibodies
Functional Studies (Func) 2 Antibodies 44 Antibodies
Immunocytochemistry (ICC) 2 Antibodies 22 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies 35 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Western Blotting (WB) 2 Antibodies
Activation (Act) 1 Antibodies
Biochemical Assay (BCA) 3 Antibodies
ELISA 79 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Immunofluorescence (IF) 30 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 29 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies

General

Antigen CD28 Antibodies
Reactivity Human, Non-Human Primate
(314), (306), (102), (38), (26), (12), (5), (5), (5), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(359), (189), (77), (42), (25), (20), (7)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD28 antibody is un-conjugated
(106), (90), (66), (41), (10), (8), (7), (7), (7), (6), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Functional Studies (Func), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
(540), (173), (113), (95), (91), (42), (41), (34), (26), (22), (13), (8), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Pubmed 7 references available
Supplier Log in to see

Product Details anti-CD28 Antibody

Target Details CD28 Application Details Handling References for anti-CD28 Antibody (ABIN638435) Images
Specificity The antibody CD28.2 reacts with CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.HLDA V., WS Code 5T CD28.05
Purification Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
Immunogen DC28.1.3.3 murine T cell hybridoma transfected with human CD28 cDNA
Clone CD28-2
Isotype IgG1

Target Details CD28

Product Details anti-CD28 Antibody Application Details Handling References for anti-CD28 Antibody (ABIN638435) Images back to top
Antigen
Alternative Name CD28 (CD28 Antibody Abstract)
Background CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.
Gene ID 940
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway

Application Details

Product Details anti-CD28 Antibody Target Details CD28 Handling References for anti-CD28 Antibody (ABIN638435) Images back to top
Application Notes Functional Application: T cell costimulation

Working concentrations should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD28 Antibody Target Details CD28 Application Details References for anti-CD28 Antibody (ABIN638435) Images back to top
Concentration 1 mg/mL
Buffer Azide free phosphate buffered saline (PBS), approx. pH 7.4.
Preservative Azide free
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.

References for anti-CD28 Antibody (ABIN638435)

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling Images back to top
Product cited in:

Scharschmidt, Wegener, Heissmeyer, Rao, Krappmann: "Degradation of Bcl10 induced by T-cell activation negatively regulates NF-kappa B signaling." in: Molecular and cellular biology, Vol. 24, Issue 9, pp. 3860-73, 2004

Jeong, Qiao, Nascimbeni, Hu, Rehermann, Murthy, Liang: "Immunization with hepatitis C virus-like particles induces humoral and cellular immune responses in nonhuman primates." in: Journal of virology, Vol. 78, Issue 13, pp. 6995-7003, 2004

Marti, Krause, Post, Lyddane, Dupont, Sadelain, King: "Negative-feedback regulation of CD28 costimulation by a novel mitogen-activated protein kinase phosphatase, MKP6." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 1, pp. 197-206, 2001

Galea-Lauri, Darling, Gan, Krivochtchapov, Kuiper, Gäken, Souberbielle, Farzaneh: "Expression of a variant of CD28 on a subpopulation of human NK cells: implications for B7-mediated stimulation of NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 1, pp. 62-70, 1999

Tazi, Moreau, Bergeron, Dominique, Hance, Soler: "Evidence that Langerhans cells in adult pulmonary Langerhans cell histiocytosis are mature dendritic cells: importance of the cytokine microenvironment." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 6, pp. 3511-5, 1999

Nunes, Klasen, Franco, Lipcey, Mawas, Bagnasco, Olive: "Signalling through CD28 T-cell activation pathway involves an inositol phospholipid-specific phospholipase C activity." in: The Biochemical journal, Vol. 293 ( Pt 3), pp. 835-42, 1993

Background publications

Nunès, Klasen, Ragueneau, Pavon, Couez, Mawas, Bagnasco, Olive: "CD28 mAbs with distinct binding properties differ in their ability to induce T cell activation: analysis of early and late activation events." in: International immunology, Vol. 5, Issue 3, pp. 311-5, 1993

Images

Product Details anti-CD28 Antibody Target Details CD28 Application Details Handling References for anti-CD28 Antibody (ABIN638435) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD28 antibody (CD28) (ABIN638435) Surface staining of human peripheral blood leukocytes with anti-human CD28 (CD28.2) p...