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anti-Human CD3D antibody for ELISA

Recommended CD3D Antibody (supplied by: Log in to see )

Antigen
CD3d Molecule, delta (CD3-TCR Complex) (CD3D) Antibodies
  • CD3-DELTA
  • T3D
  • T3d
Epitope
AA 1-105
13
5
5
5
5
4
4
4
3
1
1
1
1
Reactivity
Human
52
21
11
5
5
3
2
Host
Mouse
61
23
1
Clonality
Monoclonal
Conjugate
This CD3D antibody is un-conjugated
12
11
1
1
Minimum Detection Limit
0.039 ng/well
Application
Immunoprecipitation (IP), ELISA, Western Blotting (WB)
52
42
16
13
8
7
7
6
5
5
3
2
2
1
Supplier
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Catalog No. ABIN1997887
$ 218.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0012121415 ABIN1997889 ELISA WB Rabbit IgG AA 1-105 Log in to see 6
0.0012121415 ABIN1453089 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
0.0012121415 ABIN1997895 IP ELISA WB Rabbit IgG AA 1-105 Log in to see Polyclonal
0.0012121415 ABIN1175133 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 18-113 Log in to see Polyclonal
0.0012121415 ABIN3187952 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
0.0012121415 ABIN3183769 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
0.0012121415 ABIN560223 ELISA WB Mouse AA 22-100, partial Log in to see Polyclonal
0.0012121415 ABIN2593150 ELISA Biotin Rabbit IgG AA 18-113 Log in to see Polyclonal
0.0012121415 ABIN2593155 ELISA FITC Rabbit IgG AA 18-113 Log in to see Polyclonal
0.0012121415 ABIN2884547 ELISA WB Rabbit IgG AA 7-56 Log in to see Polyclonal
0.0012121415 ABIN2593143 ELISA Rabbit IgG AA 18-113 Log in to see Polyclonal
0.0012121415 ABIN1083989 IF ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012121415 ABIN1083990 IF ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012121415 ABIN2225815 IC IHC ELISA WB Rabbit IgG AA 18-113 Log in to see Polyclonal
0.0012121415 ABIN1175135 IHC ELISA WB Biotin Rabbit IgG Log in to see Polyclonal
0.0012121415 ABIN2561722 ELISA WB Goat IgG Internal Region Log in to see Polyclonal
0.0012121415 ABIN1997893 ELISA Rabbit IgG AA 1-105 Log in to see Polyclonal
0.0012121415 ABIN2136507 IF ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012121415 ABIN2409618 IF ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012121415 ABIN2649299 ELISA Mouse Log in to see

Similar anti-CD3D Antibodies

Application / Reactivity Human
ELISA 21 Antibodies
Flow Cytometry (FACS) 6 Antibodies
Functional Studies (Func) 3 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 6 Antibodies
Immunohistochemistry (IHC) 9 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 39 Antibodies

General

Antigen CD3d Molecule, delta (CD3-TCR Complex) (CD3D) Antibodies
Epitope AA 1-105
(13), (5), (5), (5), (5), (4), (4), (4), (3), (1), (1), (1), (1)
Reactivity Human
(52), (21), (11), (5), (5), (3), (2)
Host Mouse
(61), (23), (1)
Clonality
Monoclonal
Conjugate This CD3D antibody is un-conjugated
(12), (11), (1), (1)
Minimum Detection Limit 0.039 ng/well
Application Immunoprecipitation (IP), ELISA, Western Blotting (WB)
(52), (42), (16), (13), (8), (7), (7), (6), (5), (5), (3), (2), (2), (1)
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Product Details anti-CD3D Antibody

Target Details CD3D Application Details Handling Images
Specificity Human CD3D.
Cross-Reactivity (Details) No cross-reactivity with human cell lysate (293 cell line) in WB and ELISA
Characteristics This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant human CD3D ( rh CD3D , NP_000723.1 , Met 1 - Ala 105 ). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Purification Affinity Purified
Sterility 0.2 μm filtered
Immunogen Recombinant human CD3D protein
Isotype IgG1

Target Details CD3D

Product Details anti-CD3D Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD3D (CD3D Antibody Abstract)
Background T-cell surface glycoprotein CD3 delta chain, also known as CD3D, is a single-pass type I membrane protein. CD3D, together with CD3-gamma, CD3-epsilon and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). CD3D contains 1 ITAM domain and has been shown to interact with CD8A. Defects in CD3D cause severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T- /B+/NK+ SCID) which is a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels.
NCBI Accession NP_000723.1
Pathways TCR Signaling

Application Details

Product Details anti-CD3D Antibody Target Details CD3D Handling Images back to top
Application Notes Direct ELISA - This antibody can be used at 0.5-1
Restrictions For Research Use only

Handling

Product Details anti-CD3D Antibody Target Details CD3D Application Details Images back to top
Buffer PBS with 5% trehalose
Preservative Without preservative
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C/-20 °C/-80 °C
Storage Comment This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -70°C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly.
Expiry Date 12 months

Images

Product Details anti-CD3D Antibody Target Details CD3D Application Details Handling back to top
Supplier Images
SDS-PAGE (SDS) image for anti-CD3D antibody (CD3d Molecule, delta (CD3-TCR Complex)) (AA 1-105) (ABIN1997887) anti-CD3d Molecule, delta (CD3-TCR Complex) (CD3D) (AA 1-105) antibody