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anti-Human CD3D antibody for Immunoprecipitation

Recommended CD3D Antibody (supplied by: Log in to see )

Antigen
CD3d Molecule, delta (CD3-TCR Complex) (CD3D) Antibodies
  • CD3-DELTA
  • T3D
  • T3d
Epitope
AA 1-105
13
5
5
5
5
4
4
4
3
1
1
1
1
Reactivity
Human
50
21
11
5
5
3
2
Host
Rabbit
59
23
1
Clonality
Polyclonal
Conjugate
This CD3D antibody is un-conjugated
12
11
1
1
Minimum Detection Limit
0.00245 ng/well
Application
Immunoprecipitation (IP), ELISA, Western Blotting (WB)
51
42
15
12
7
7
7
6
5
4
2
2
2
1
Supplier
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Catalog No. ABIN1997895
$ 218.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089029176 ABIN1997887 IP ELISA WB Mouse IgG1 AA 1-105 Log in to see
0.00089029176 ABIN1997891 IP WB Rabbit IgG AA 1-105 Log in to see 138

Similar anti-CD3D Antibodies

Application / Reactivity Human
ELISA 21 Antibodies
Flow Cytometry (FACS) 5 Antibodies
Functional Studies (Func) 2 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 6 Antibodies
Immunohistochemistry (IHC) 8 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 38 Antibodies

General

Antigen CD3d Molecule, delta (CD3-TCR Complex) (CD3D) Antibodies
Epitope AA 1-105
(13), (5), (5), (5), (5), (4), (4), (4), (3), (1), (1), (1), (1)
Reactivity Human
(50), (21), (11), (5), (5), (3), (2)
Host Rabbit
(59), (23), (1)
Clonality
Polyclonal
Conjugate This CD3D antibody is un-conjugated
(12), (11), (1), (1)
Minimum Detection Limit 0.00245 ng/well
Application Immunoprecipitation (IP), ELISA, Western Blotting (WB)
(51), (42), (15), (12), (7), (7), (7), (6), (5), (4), (2), (2), (2), (1)
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Product Details anti-CD3D Antibody

Target Details CD3D Application Details Handling Images
Specificity Human CD3D
Characteristics Produced in rabbits immunized with purified, recombinant Human CD3D (rh CD3D, NP_000723.1, Met 1-Ala 105). CD3D specific IgG was purified by human CD3D affinity chromatography.
Purification Affinity Purified
Sterility 0.2 μm filtered
Isotype IgG

Target Details CD3D

Product Details anti-CD3D Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD3D (CD3D Antibody Abstract)
Background T-cell surface glycoprotein CD3 delta chain, also known as CD3D, is a single-pass type I membrane protein. CD3D, together with CD3-gamma, CD3-epsilon and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The majority of T cell receptor (TCR) complexes in mice and humans consist of a heterodimer of polymorphic TCRalpha and beta chains along with invariant CD3gamma, delta, epsilon, and zeta chains. CD3 chains are present as CD3gammaepsilon, deltaepsilon, and zetazeta dimers in the receptor complex and play critical roles in the antigen receptor assembly, transport to the cell surface, and the receptor-mediated signal transduction. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). CD3D contains 1 ITAM domain and has been shown to interact with CD8A. In the mouse, knockout of CD3delta allows some degree of T lymphocyte differentiation since mature CD4 and CD8 as well as TCRgammadelta T lymphocytes are observed in the periphery. In contrast, deleterious mutation of the CD3delta encoding gene in the human leads to a severe combined immunodeficiency characterised by the complete absence of mature T cell subpopulations including TCRalpha/beta and TCRgamma/delta. Defects in CD3D cause severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T-/B+/NK+ SCID) which is a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. In humans the absence of CD3 delta results in a complete arrest in thymocyte development at the stage of double negative to double positive transition and the development of gamma delta T-cell receptor- positive T cells is also impaired.
NCBI Accession NP_000723.1
Pathways TCR Signaling

Application Details

Product Details anti-CD3D Antibody Target Details CD3D Handling Images back to top
Application Notes ELISA ELISA: 0.5-1.0 μg/mL This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect Human CD3D. The detection limit for Human CD3D is 0.00245 ng/well.
Restrictions For Research Use only

Handling

Product Details anti-CD3D Antibody Target Details CD3D Application Details Images back to top
Buffer PBS with 5% trehalose
Preservative Without preservative
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C/-20 °C/-80 °C
Storage Comment This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -70°C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly.
Expiry Date 12 months

Images

Product Details anti-CD3D Antibody Target Details CD3D Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-CD3D antibody (CD3d Molecule, delta (CD3-TCR Complex)) (AA 1-105) (ABIN1997895) anti-CD3d Molecule, delta (CD3-TCR Complex) (CD3D) (AA 1-105) antibody
Immunoprecipitation (IP) image for anti-CD3D antibody (CD3d Molecule, delta (CD3-TCR Complex)) (AA 1-105) (ABIN1997895) anti-CD3d Molecule, delta (CD3-TCR Complex) (CD3D) (AA 1-105) antibody (Image 2)