Human Monoclonal CD3E Primary Antibody for CyTOF, FACS - ABIN4900708
Jones, Stumhofer, Foster, Twohig, Hertzog, Topley, Williams, Hunter, Jenkins, Wang, Jones: Naive and activated T cells display differential responsiveness to TL1A that affects Th17 generation, maintenance, and proliferation. in FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2011
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD3e / CD3 epsilon (rh CD3e / CD3 epsilon, ABIN2003682 NP_000724.1, Met1-Asp126, and conjugated with PE under optimum conditions, the unreacted PE was removed.
Recombinant Human CD3e / CD3 epsilon protein (ABIN2003682)
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal
This antibody is stable for 12 months from date of receipt when stored at 2°C-8°C. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
This antibody performed well and is comparable to anti-CD3-FITC from BD Pharmingen (see data below) in terms of detecting a similar percentage of cells. The recommended amount of reagent to use per test is 1ul. You can see that even with using a small amount that the stained cells are very bright and are almost off scale. Even the negative peak has some spread to it. This antibody will need to be diluted further to obtain optimum staining.