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anti-Human CD3E antibody for Flow Cytometry

Recommended CD3E Antibody (supplied by: Log in to see )

Antigen
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
  • AI504783
  • CD3
  • cd3e
  • CD3E
  • CD3epsilon
  • T3E
  • t3e
  • T3e
  • TCRE
  • tcre
Reactivity
Human
524
412
124
90
65
45
44
43
43
43
30
26
25
24
22
22
21
7
6
2
2
2
2
1
1
Host
Mouse
359
225
211
79
35
21
1
Clonality
Monoclonal
Conjugate
This CD3E antibody is conjugated to PE
97
81
70
40
20
20
19
19
17
16
16
15
14
13
13
13
13
13
13
13
11
11
7
7
6
5
5
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
691
298
218
168
128
92
88
85
85
34
25
10
9
6
4
3
3
2
2
1
1
1
1
1
1
1
1
Supplier
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Supplier Product No.
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Available images

Catalog No. ABIN2213320
$ 198.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30391768 ABIN638428 Func FACS IP Mouse IgG2a Dimer gamma-epsilon, Dimer delta-epsilon Log in to see MEM-57 12
0.30391768 ABIN638434 Func IHC (fro) FACS IP Mouse IgG1 Log in to see UCHT1 12
0.30391768 ABIN489928 Func FACS IHC (fro) IP APC Mouse IgG1 Log in to see UCHT1 11
0.30391768 ABIN489929 Func FACS IHC (fro) IP PerCP Mouse IgG1 Log in to see UCHT1 9
0.30391768 ABIN969024 FACS ELISA WB Mouse IgG1 Log in to see 4-00E-02 5
0.30391768 ABIN94060 FACS Biotin Mouse IgG2a Dimer gamma-epsilon, Dimer delta-epsilon Log in to see MEM-57 18
0.30391768 ABIN775704 Func IHC (fro) FACS IP Mouse IgG1 Log in to see UCHT1 9
0.30391768 ABIN302049 FACS PerCP Mouse IgG2a Dimer gamma-epsilon, Dimer delta-epsilon Log in to see MEM-57 17
0.30391768 ABIN94062 FACS PE Mouse IgG2a Dimer gamma-epsilon, Dimer delta-epsilon Log in to see MEM-57 19
0.30391768 ABIN94063 FACS APC Mouse IgG2a Dimer gamma-epsilon, Dimer delta-epsilon Log in to see MEM-57 19
0.30391768 ABIN94061 FACS FITC Mouse IgG2a Dimer gamma-epsilon, Dimer delta-epsilon Log in to see MEM-57 18
0.30391768 ABIN302021 FACS FITC Mouse IgG1 Log in to see UCHT1 10
0.30391768 ABIN302043 FACS PE Mouse IgG1 Log in to see UCHT1 10
0.30391768 ABIN2662638 FACS PE Mouse IgG1 kappa Log in to see APA1-1 10
0.30391768 ABIN2661400 FACS IF IHC FITC Mouse IgG1 kappa Log in to see APA1-1 10
0.30391768 ABIN2664657 FACS Mouse IgG1 kappa Log in to see APA1-1 10
0.30391768 ABIN2168150 FACS PE Mouse IgG1 Log in to see
0.30391768 ABIN2168151 FACS PE Mouse IgG1 Log in to see
0.30391768 ABIN2168148 FACS APC Mouse IgG1 Log in to see
0.30391768 ABIN2168149 FACS APC Mouse IgG1 Log in to see

Top referenced anti-CD3E antibody for Flow Cytometry

Similar anti-CD3E Antibodies

Application / Reactivity Human
Activation (Act) 1 Antibodies
Agonist (Agon) 1 Antibodies
Blocking Reagent (BR) 3 Antibodies
ELISA 86 Antibodies
Flow Cytometry (FACS) 225 Antibodies
Functional Studies (Func) 8 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 85 Antibodies
Immunofluorescence (IF) 97 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 82 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 57 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 53 Antibodies
Immunoprecipitation (IP) 69 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies

General

Antigen CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
Reactivity Human
(524), (412), (124), (90), (65), (45), (44), (43), (43), (43), (30), (26), (25), (24), (22), (22), (21), (7), (6), (2), (2), (2), (2), (1), (1)
Host Mouse
(359), (225), (211), (79), (35), (21), (1)
Clonality
Monoclonal
Conjugate This CD3E antibody is conjugated to PE
(97), (81), (70), (40), (20), (20), (19), (19), (17), (16), (16), (15), (14), (13), (13), (13), (13), (13), (13), (13), (11), (11), (7), (7), (6), (5), (5), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(691), (298), (218), (168), (128), (92), (88), (85), (85), (34), (25), (10), (9), (6), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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Target Details CD3E

Product Details anti-CD3E Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD3E (CD3E Antibody Abstract)
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD3E Antibody Target Details CD3E Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD3E Antibody Target Details CD3E Application Details Images back to top
Handling Advice Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal
Storage 4 °C
Storage Comment This antibody is stable for 12 months from date of receipt when stored at 2 °C-8 °C.
Expiry Date 12 months

Images

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex))  (PE) (ABIN2213320) anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) antibody (PE)
'Independent Validation' Badge
Lot Number HG08FE2109
Method validated Flow Cytometry
Positive Control peripheral blood mononuclear cells
Negative Control peripheral blood mononuclear cells
Notes This antibody performed well and is comparable to anti-CD3-FITC from BD Pharmingen (see data below) in terms of detecting a similar percentage of cells. The recommended amount of reagent to use per test is 1ul. You can see that even with using a small amount that the stained cells are very bright and are almost off scale. Even the negative peak has some spread to it. This antibody will need to be diluted further to obtain optimum staining.
Primary Antibody
  • Antigen: human CD3 (CD3e)
  • Catalog number: ABIN2104932
  • Supplier: Log in to see
  • Supplier catalog number: Log in to see
  • Lot number: HG08FE2109
  • Dilution: 1 µl in 100 µL 1X PBS containing 0.5% BSA
Controls
  • Positive control: peripheral blood mononuclear cells stained with anti-CD3
  • Negative control: peripheral blood mononuclear cells that are CD3 negative and unstained cells
  • Isotype Control: None
  • Secondary Only control: None
Protocol
  • 1. 10mls of blood (collected in EDTA) was diluted to 20mls with PBS + 0.5% BSA
  • 2. 10mls of diluted blood was overlaid onto 5mls of ficoll.
  • 3. Spin for 20 minutes at 1500 rpm with break off.
  • 4. The buffy coat layer was collected and wash with PBS/BSA.
  • 5. Residual red blood cells were lysed by resuspending the cells in 1ml ammonium chloride for 5-10 minutes at room temperature.
  • 6. The cells were washed with PBS/BSA, counted and resuspended at 10x10^6 cells/ml.
  • 7. 100ul of cells (1x10^6) were added to their respective tubes.
  • 8. Add 1ul of anti-CD3-PE (Sino Biological) or 20ul of anti-CD3-FITC (BD Pharmingen) to the appropriate tubes and incubate for 20 minutes on ice.
  • 9. Add 1ml PBS/BSA. Spin the cells down and resuspend in 0.5ml PBS/BSA.
  • 10. Analyze
Validation Images
Flow Cytometry validation image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) antibody (PE) (ABIN2104932) Figure 1: Histogram of peripheral blood lymphocytes stained with anti-CD3-FITC (red),...
Flow Cytometry validation image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) antibody (PE) (ABIN2104932) Figure 2: Histogram of peripheral blood lymphocytes stained with anti-CD3-FITC from B...
Flow Cytometry validation image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) antibody (PE) (ABIN2104932) Figure 3: Dot plot of peripheral blood lymphocytes stained with anti-CD4-FITC (x-axis...