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anti-Human CD3E antibody for Immunofluorescence

Recommended CD3E Antibody (supplied by: Log in to see )

Antigen
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
  • AI504783
  • CD3
  • cd3e
  • CD3E
  • CD3epsilon
  • T3E
  • t3e
  • T3e
  • TCRE
  • tcre
Reactivity
Human, Mouse (Murine), Rat (Rattus)
392
243
61
45
7
6
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
Host
Rabbit
204
161
155
77
33
21
1
Clonality
Polyclonal
Conjugate
This CD3E antibody is un-conjugated
87
67
60
40
17
15
11
10
7
7
5
5
5
5
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
460
176
174
132
68
64
53
45
34
24
20
10
9
6
4
3
3
2
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN2404594
$ 588.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010850558 ABIN1497222 IF IHC (p) WB Mouse IgG2b Log in to see 3E10
0.0010850558 ABIN2661400 FACS IF IHC FITC Mouse IgG1 kappa Log in to see APA1-1 10
0.0010850558 ABIN2661401 FACS IF IHC FITC Mouse IgG1 kappa Log in to see APA1-1 10
0.0010850558 ABIN2879900 IF Rabbit IgG Log in to see Polyclonal
0.0010850558 ABIN251171 ICC FACS IHC (fro) IF IP IHC RID Mouse IgG1 kappa non-glycosylated Log in to see UCHT1
0.0010850558 ABIN440731 ICC FACS IHC (fro) IF IP IHC WB Mouse IgG1 Log in to see APA1-1
0.0010850558 ABIN2225120 IF IHC ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
0.0010850558 ABIN2694985 IF IHC WB Rabbit IgG Log in to see Polyclonal
0.0010850558 ABIN2225119 IF IHC WB Rabbit IgG C-Term Log in to see Polyclonal
0.0010850558 ABIN1854857 ICC IF IHC WB Rabbit IgG N-Term Log in to see Polyclonal
0.0010850558 ABIN2671183 IF IHC WB Mouse IgG2b Log in to see OTIE10
0.0010850558 ABIN2990265 ICC IF IHC WB Rabbit IgG Log in to see Polyclonal
0.0010850558 ABIN1882847 IF IHC Rabbit C-Term Log in to see Polyclonal
0.0010850558 ABIN1882849 IF IHC WB Rabbit Log in to see Polyclonal

Top referenced anti-CD3E antibody for Immunofluorescence

Similar anti-CD3E Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 10 Antibodies 14 Antibodies
Flow Cytometry (FACS) 2 Antibodies 338 Antibodies
Immunocytochemistry (ICC) 3 Antibodies 17 Antibodies
Immunofluorescence (IF) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 7 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 10 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Western Blotting (WB) 27 Antibodies
Blocking Reagent (BR) 6 Antibodies
Cytotoxicity Test (CyTox) 3 Antibodies
ELISA (Capture) 6 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 2 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Functional Studies (Func) 17 Antibodies

General

Antigen CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(392), (243), (61), (45), (7), (6), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Host Rabbit
(204), (161), (155), (77), (33), (21), (1)
Clonality
Polyclonal
Conjugate This CD3E antibody is un-conjugated
(87), (67), (60), (40), (17), (15), (11), (10), (7), (7), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(460), (176), (174), (132), (68), (64), (53), (45), (34), (24), (20), (10), (9), (6), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD3E Antibody

Target Details CD3E Application Details Handling Images
Purification Antigen affinity purification
Immunogen Recombinant protein of human CD3E
Isotype IgG

Target Details CD3E

Product Details anti-CD3E Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD3E (CD3E Antibody Abstract)
Background When T cells encounter antigens via the T cell receptor (TCR), information about the quantity and quality of antigens is relayed to the intracellular signal transduction machinery (1). This activation process depends mainly on CD3 (Cluster of Differentiation 3), a multiunit protein complex that directly associates with the TCR. CD3 is composed of four polypeptides: ζ, γ, ε and δ. Each of these polypeptides contains at least one immunoreceptor tyrosine-based activation motif (ITAM) (2). Engagement of TCR complex with foreign antigens induces tyrosine phosphorylation in the ITAM motifs and phosphorylated ITAMs function as docking sites for signaling molecules such as ZAP-70 and p85 subunit of PI-3 kinase (3,4). TCR ligation also induces a conformational change in CD3ε, such that a proline-region is exposed and then associates with the adapter protein Nck (5).
Synonyms: CD3ε, FLJ18683, T3E, TCRE,
Molecular Weight Observed MW: 23kDa
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD3E Antibody Target Details CD3E Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD3E Antibody Target Details CD3E Application Details Images back to top
Format Liquid
Handling Advice Avoid freeze / thaw cycles.
Storage -20 °C/-80 °C
Storage Comment Store at -20°C (regular) and -80°C (long term).

Images

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (ABIN2404594) Immunofluorescence analysis of U2OS cell using CD3E antibody. Blue: DAPI for nuclear ...
Western Blotting (WB) image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (ABIN2404594) Western blot analysis of extracts of Jurkat cell lines, using CD3E antibody.