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anti-Human CD3E antibody for Western Blotting

Recommended CD3E Antibody (supplied by: Log in to see )

Antigen
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
  • AI504783
  • CD3
  • cd3e
  • CD3E
  • CD3epsilon
  • T3E
  • t3e
  • T3e
  • TCRE
  • tcre
Reactivity
Human
393
243
61
46
7
6
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
Host
Mouse
204
160
156
77
33
21
1
Clonality
Monoclonal
Conjugate
This CD3E antibody is un-conjugated
87
67
60
40
17
15
11
10
7
7
5
5
5
5
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Flow Cytometry (FACS), ELISA, Western Blotting (WB)
459
176
174
133
67
64
53
46
34
24
20
10
9
6
4
3
3
2
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN969024
$ 393.80
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12303223 ABIN2787934 WB Rabbit Middle Region Log in to see Polyclonal
0.12303223 ABIN657454 WB Rabbit Ig AA 154-182, C-Term Log in to see Polyclonal
0.12303223 ABIN3043805 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 23-207 Log in to see Polyclonal
0.12303223 ABIN2705793 WB Rabbit Center Log in to see Polyclonal
0.12303223 ABIN1871626 IHC WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN396257 WB Mouse IgG2a kappa AA 23-208 Log in to see 4C1
0.12303223 ABIN2159904 WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
0.12303223 ABIN3022492 IHC WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN931331 FACS ELISA WB Mouse IgG1 Log in to see 4E2
0.12303223 ABIN514135 PLA ELISA WB Mouse IgG2a kappa AA 23-207, full length Log in to see 4C1
0.12303223 ABIN3187953 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
0.12303223 ABIN929127 WB Rabbit Middle Region Log in to see Polyclonal
0.12303223 ABIN2425325 WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN2404594 IF IHC WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN1845772 FACS ELISA WB Mouse IgG1 Log in to see
0.0011806369 ABIN457415 ICC FACS IHC (fro) IP WB Mouse IgG1 Activation Site Log in to see APA1-1 7
0.0011806369 ABIN1497222 IF IHC (p) WB Mouse IgG2b Log in to see 3E10
0.0011806369 ABIN1385274 IF (p) IHC (p) WB Rabbit IgG AA 75-125 Log in to see Polyclonal
0.0011806369 ABIN672921 IF (p) IHC (p) WB Rabbit IgG AA 132-170 Log in to see Polyclonal
0.0011806369 ABIN211097 ICC FACS IP WB Mouse IgG1 Log in to see

Top referenced anti-CD3E antibody for Western Blotting

Similar anti-CD3E Antibodies

Application / Reactivity Human
Activation (Act) 1 Antibodies
Agonist (Agon) 1 Antibodies
Blocking Reagent (BR) 3 Antibodies
ELISA 66 Antibodies
Flow Cytometry (FACS) 84 Antibodies
Functional Studies (Func) 7 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 13 Antibodies
Immunofluorescence (IF) 15 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 48 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 25 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 32 Antibodies
Immunoprecipitation (IP) 32 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies

General

Antigen CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
Reactivity Human
(393), (243), (61), (46), (7), (6), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Host Mouse
(204), (160), (156), (77), (33), (21), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD3E antibody is un-conjugated
(87), (67), (60), (40), (17), (15), (11), (10), (7), (7), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), ELISA, Western Blotting (WB)
(459), (176), (174), (133), (67), (64), (53), (46), (34), (24), (20), (10), (9), (6), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-CD3E Antibody

Target Details CD3E Application Details Handling References for anti-CD3E Antibody (ABIN969024) Images
Purification Ascites
Immunogen Purified recombinant fragment of CD3E expressed in E. coli.
Clone 4-00E-02
Isotype IgG1

Target Details CD3E

Product Details anti-CD3E Antibody Application Details Handling References for anti-CD3E Antibody (ABIN969024) Images back to top
Antigen
Alternative Name CD3E (CD3E Antibody Abstract)
Background The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.
Molecular Weight 23 kDa
Gene ID 916
HGNC 916
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD3E Antibody Target Details CD3E Handling References for anti-CD3E Antibody (ABIN969024) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-CD3E Antibody Target Details CD3E Application Details References for anti-CD3E Antibody (ABIN969024) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-CD3E Antibody (ABIN969024)

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling Images back to top
Product cited in:

Boskovic, Arnold, Stilion, Gordon, Sim, Rivera-Calzada, Wienke, Isacke, Martinez-Pomares, Llorca: "Structural model for the mannose receptor family uncovered by electron microscopy of Endo180 and the mannose receptor." in: The Journal of biological chemistry, Vol. 281, Issue 13, pp. 8780-7, 2006

Urano, Iiduka, Sugiyama, Akiyama, Uzawa, Matsumoto, Kawasaki, Tashiro: "Involvement of the mouse Prp19 gene in neuronal/astroglial cell fate decisions." in: The Journal of biological chemistry, Vol. 281, Issue 11, pp. 7498-514, 2006

Xu, Call, Wucherpfennig: "A membrane-proximal tetracysteine motif contributes to assembly of CD3deltaepsilon and CD3gammaepsilon dimers with the T cell receptor." in: The Journal of biological chemistry, Vol. 281, Issue 48, pp. 36977-84, 2006

Li: "Therapeutic biology: checkpoint pathway activation therapy, HIV Tat, and transkingdom RNA interference." in: Journal of cellular physiology, Vol. 209, Issue 3, pp. 695-700, 2006

Tao, Wollscheid, OBrien, Eng, Li, Bodenmiller, Watts, Hood, Aebersold: "Quantitative phosphoproteome analysis using a dendrimer conjugation chemistry and tandem mass spectrometry." in: Nature methods, Vol. 2, Issue 8, pp. 591-8, 2005

Images

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling References for anti-CD3E Antibody (ABIN969024) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (ABIN969024) Figure 1: Flow cytometric analysis of Jurkat cells using CD3E mouse mAb (green) and n...
Western Blotting (WB) image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (ABIN969024) Figure 2: Western blot analysis using CD3E mouse mAb against Jurkat (1) cell lysate.