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anti-Human CD3E antibody for Western Blotting

Recommended CD3E Antibody (supplied by: Log in to see )

Antigen
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
  • AI504783
  • CD3
  • cd3e
  • CD3E
  • CD3epsilon
  • T3E
  • t3e
  • T3e
  • TCRE
  • tcre
Reactivity
Human
480
420
108
74
48
28
27
26
26
26
21
13
9
8
7
7
6
5
5
2
2
2
2
1
1
Host
Mouse
383
213
211
74
19
13
1
Clonality
Monoclonal
Conjugate
This CD3E antibody is un-conjugated
95
81
66
39
21
20
19
19
17
17
15
15
15
12
12
12
12
12
12
11
10
10
10
7
6
6
4
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
Application
Flow Cytometry (FACS), ELISA, Western Blotting (WB)
684
278
211
166
110
90
86
83
75
38
25
10
9
9
6
4
3
3
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN969024
$ 393.80
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.124960825 ABIN2787934 WB Rabbit Middle Region Log in to see Polyclonal
0.124960825 ABIN657454 WB Rabbit Ig AA 154-182, C-Term Log in to see Polyclonal
0.124960825 ABIN3043805 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 23-207 Log in to see Polyclonal
0.124960825 ABIN2705793 WB Rabbit Center Log in to see Polyclonal
0.124960825 ABIN1871626 IF IHC WB Rabbit IgG Log in to see Polyclonal
0.124960825 ABIN396257 WB Mouse IgG2a kappa AA 23-208 Log in to see 4C1
0.124960825 ABIN2159904 WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
0.124960825 ABIN151493 IHC (p) IHC WB Rabbit Log in to see Polyclonal
0.124960825 ABIN3022492 IHC WB Rabbit IgG Log in to see Polyclonal
0.124960825 ABIN3187953 ELISA IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal
0.124960825 ABIN1858299 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 28-185 Log in to see Polyclonal
0.124960825 ABIN514135 PLA ELISA WB Mouse IgG2a kappa AA 23-207, full length Log in to see 4C1
0.124960825 ABIN931331 FACS ELISA WB Mouse IgG1 Log in to see 4E2
0.124960825 ABIN2801427 WB Rabbit Log in to see Polyclonal
0.124960825 ABIN2404594 IF IHC WB Rabbit IgG Log in to see Polyclonal
0.124960825 ABIN441172 DB WB Rabbit IgG Log in to see Polyclonal
0.124960825 ABIN929127 WB Rabbit Middle Region Log in to see Polyclonal
0.124960825 ABIN4291564 IF IHC WB Mouse IgG2b Log in to see 3-00E10
0.124960825 ABIN4291554 ELISA WB Mouse IgG1 kappa AA 23-126 Log in to see 3H3
0.124960825 ABIN3030389 ELISA WB Rabbit Ig Fraction AA 154-182 Log in to see Polyclonal

Top referenced anti-CD3E antibody for Western Blotting

Similar anti-CD3E Antibodies

Application / Reactivity Human
Activation (Act) 1 Antibodies
Agonist (Agon) 1 Antibodies
Blocking Reagent (BR) 3 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 89 Antibodies
Flow Cytometry (FACS) 245 Antibodies
Functional Studies (Func) 15 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 76 Antibodies
Immunofluorescence (IF) 79 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 24 Antibodies
Immunohistochemistry (IHC) 80 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 8 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 52 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 54 Antibodies
Immunoprecipitation (IP) 62 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies

General

Antigen CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
Reactivity Human
(480), (420), (108), (74), (48), (28), (27), (26), (26), (26), (21), (13), (9), (8), (7), (7), (6), (5), (5), (2), (2), (2), (2), (1), (1)
Host Mouse
(383), (213), (211), (74), (19), (13), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD3E antibody is un-conjugated
(95), (81), (66), (39), (21), (20), (19), (19), (17), (17), (15), (15), (15), (12), (12), (12), (12), (12), (12), (11), (10), (10), (10), (7), (6), (6), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Flow Cytometry (FACS), ELISA, Western Blotting (WB)
(684), (278), (211), (166), (110), (90), (86), (83), (75), (38), (25), (10), (9), (9), (6), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-CD3E Antibody

Target Details CD3E Application Details Handling References for anti-CD3E Antibody (ABIN969024) Images
Purification Ascites
Immunogen Purified recombinant fragment of CD3E expressed in E. coli.
Clone 4-00E-02
Isotype IgG1

Target Details CD3E

Product Details anti-CD3E Antibody Application Details Handling References for anti-CD3E Antibody (ABIN969024) Images back to top
Antigen
Alternative Name CD3E (CD3E Antibody Abstract)
Background The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.
Molecular Weight 23 kDa
Gene ID 916
HGNC 916
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD3E Antibody Target Details CD3E Handling References for anti-CD3E Antibody (ABIN969024) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-CD3E Antibody Target Details CD3E Application Details References for anti-CD3E Antibody (ABIN969024) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-CD3E Antibody (ABIN969024)

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling Images back to top
Product cited in:

Boskovic, Arnold, Stilion, Gordon, Sim, Rivera-Calzada, Wienke, Isacke, Martinez-Pomares, Llorca: "Structural model for the mannose receptor family uncovered by electron microscopy of Endo180 and the mannose receptor." in: The Journal of biological chemistry, Vol. 281, Issue 13, pp. 8780-7, 2006

Urano, Iiduka, Sugiyama, Akiyama, Uzawa, Matsumoto, Kawasaki, Tashiro: "Involvement of the mouse Prp19 gene in neuronal/astroglial cell fate decisions." in: The Journal of biological chemistry, Vol. 281, Issue 11, pp. 7498-514, 2006

Xu, Call, Wucherpfennig: "A membrane-proximal tetracysteine motif contributes to assembly of CD3deltaepsilon and CD3gammaepsilon dimers with the T cell receptor." in: The Journal of biological chemistry, Vol. 281, Issue 48, pp. 36977-84, 2006

Li: "Therapeutic biology: checkpoint pathway activation therapy, HIV Tat, and transkingdom RNA interference." in: Journal of cellular physiology, Vol. 209, Issue 3, pp. 695-700, 2006

Tao, Wollscheid, OBrien, Eng, Li, Bodenmiller, Watts, Hood, Aebersold: "Quantitative phosphoproteome analysis using a dendrimer conjugation chemistry and tandem mass spectrometry." in: Nature methods, Vol. 2, Issue 8, pp. 591-8, 2005

Images

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling References for anti-CD3E Antibody (ABIN969024) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (ABIN969024) Figure 1: Flow cytometric analysis of Jurkat cells using CD3E mouse mAb (green) and n...
Western Blotting (WB) image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (ABIN969024) Figure 2: Western blot analysis using CD3E mouse mAb against Jurkat (1) cell lysate.