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anti-Human CD3E antibody for Western Blotting

Recommended CD3E Antibody (supplied by: Log in to see )

Antigen
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
  • cd3e
  • CD3E
  • t3e
  • tcre
  • AI504783
  • CD3
  • CD3epsilon
  • T3e
  • T3E
  • TCRE
  • CD3 epsilon
  • CD3e molecule, epsilon (CD3-TCR complex)
  • CD3 antigen, epsilon polypeptide
  • CD3 molecule, epsilon
  • cd3e
  • CD3E
  • Cd3e
Reactivity
Human
491
475
118
75
49
30
28
28
28
28
21
13
9
9
8
7
6
5
5
2
2
2
2
1
1
Host
Mouse
444
213
210
80
20
15
2
Clonality
Monoclonal
Conjugate
This CD3E antibody is un-conjugated
101
85
72
39
23
22
21
21
20
18
17
17
17
14
14
14
14
12
12
12
12
12
7
7
6
6
4
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
Application
Flow Cytometry (FACS), ELISA, Western Blotting (WB)
742
317
219
196
147
138
114
95
90
34
25
18
13
10
9
9
6
4
3
3
3
2
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN969024
$ 393.80
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.959052 ABIN2787934 WB Rabbit Middle Region Log in to see Polyclonal
2.959052 ABIN657454 WB Rabbit Ig AA 154-182, C-Term Log in to see Polyclonal
2.959052 ABIN3043805 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 23-207 Log in to see Polyclonal
2.959052 ABIN2705793 WB Rabbit Center Log in to see Polyclonal
2.959052 ABIN3187953 ELISA IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal
2.959052 ABIN2801427 WB Rabbit Log in to see Polyclonal
2.959052 ABIN1871626 IF IHC WB Rabbit IgG Log in to see Polyclonal
2.959052 ABIN4291554 ELISA WB Mouse IgG1 kappa AA 23-126 Log in to see 3H3
2.959052 ABIN151493 IHC (p) IHC WB Rabbit Log in to see Polyclonal
2.959052 ABIN396257 WB Mouse IgG2a kappa AA 23-208 Log in to see 4C1
2.959052 ABIN2159904 WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
2.959052 ABIN441172 WB Rabbit IgG Log in to see Polyclonal
2.959052 ABIN4291564 IF IHC WB Mouse IgG2b Log in to see 3E10
2.959052 ABIN4291552 IHC WB Mouse IgG1 Log in to see CL1466
2.959052 ABIN4291553 IHC WB Mouse IgG1 Log in to see CL1497
2.959052 ABIN3022492 IHC WB Rabbit IgG Log in to see Polyclonal
2.959052 ABIN4291521 FACS ICC IF IP WB Rabbit Log in to see Polyclonal
2.959052 ABIN3030389 ELISA WB Rabbit Ig Fraction AA 154-182 Log in to see Polyclonal
2.959052 ABIN1858299 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 28-185 Log in to see Polyclonal
2.959052 ABIN3026772 FACS IP WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-CD3E antibody for Western Blotting

Similar anti-CD3E Antibodies

Application / Reactivity Human
Activation (Act) 3 Antibodies
Agonist (Agon) 1 Antibodies
Blocking Reagent (BR) 3 Antibodies
Cytometry by Time of Flight (CyTOF) 9 Antibodies
ELISA 89 Antibodies
Flow Cytometry (FACS) 287 Antibodies
Functional Studies (Func) 15 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 105 Antibodies
Immunofluorescence (IF) 114 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 82 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 8 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 57 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 94 Antibodies
Immunoprecipitation (IP) 51 Antibodies

General

Antigen CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
Reactivity Human
(491), (475), (118), (75), (49), (30), (28), (28), (28), (28), (21), (13), (9), (9), (8), (7), (6), (5), (5), (2), (2), (2), (2), (1), (1)
Host Mouse
(444), (213), (210), (80), (20), (15), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD3E antibody is un-conjugated
(101), (85), (72), (39), (23), (22), (21), (21), (20), (18), (17), (17), (17), (14), (14), (14), (14), (12), (12), (12), (12), (12), (7), (7), (6), (6), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Flow Cytometry (FACS), ELISA, Western Blotting (WB)
(742), (317), (219), (196), (147), (138), (114), (95), (90), (34), (25), (18), (13), (10), (9), (9), (6), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-CD3E Antibody

Target Details CD3E Application Details Handling References for anti-CD3E Antibody (ABIN969024) Images
Purification Ascites
Immunogen Purified recombinant fragment of CD3E expressed in E. coli.
Clone 4-00E-02
Isotype IgG1

Target Details CD3E

Product Details anti-CD3E Antibody Application Details Handling References for anti-CD3E Antibody (ABIN969024) Images back to top
Antigen
Alternative Name CD3E (CD3E Antibody Abstract)
Background The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.
Molecular Weight 23 kDa
Gene ID 916
HGNC 916
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD3E Antibody Target Details CD3E Handling References for anti-CD3E Antibody (ABIN969024) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-CD3E Antibody Target Details CD3E Application Details References for anti-CD3E Antibody (ABIN969024) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-CD3E Antibody (ABIN969024)

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling Images back to top
Product cited in:

Boskovic, Arnold, Stilion, Gordon, Sim, Rivera-Calzada, Wienke, Isacke, Martinez-Pomares, Llorca: "Structural model for the mannose receptor family uncovered by electron microscopy of Endo180 and the mannose receptor." in: The Journal of biological chemistry, Vol. 281, Issue 13, pp. 8780-7, 2006

Urano, Iiduka, Sugiyama, Akiyama, Uzawa, Matsumoto, Kawasaki, Tashiro: "Involvement of the mouse Prp19 gene in neuronal/astroglial cell fate decisions." in: The Journal of biological chemistry, Vol. 281, Issue 11, pp. 7498-514, 2006

Xu, Call, Wucherpfennig: "A membrane-proximal tetracysteine motif contributes to assembly of CD3deltaepsilon and CD3gammaepsilon dimers with the T cell receptor." in: The Journal of biological chemistry, Vol. 281, Issue 48, pp. 36977-84, 2006

Li: "Therapeutic biology: checkpoint pathway activation therapy, HIV Tat, and transkingdom RNA interference." in: Journal of cellular physiology, Vol. 209, Issue 3, pp. 695-700, 2006

Tao, Wollscheid, OBrien, Eng, Li, Bodenmiller, Watts, Hood, Aebersold: "Quantitative phosphoproteome analysis using a dendrimer conjugation chemistry and tandem mass spectrometry." in: Nature methods, Vol. 2, Issue 8, pp. 591-8, 2005

Images

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling References for anti-CD3E Antibody (ABIN969024) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (ABIN969024) Figure 1: Flow cytometric analysis of Jurkat cells using CD3E mouse mAb (green) and n...
Western Blotting (WB) image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (ABIN969024) Figure 2: Western blot analysis using CD3E mouse mAb against Jurkat (1) cell lysate.