anti-Mouse (Murine) CD3E antibody for ELISpot (Capture)

Recommended CD3E Antibody (supplied by: Log in to see )

Antigen
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
  • cd3e
  • CD3E
  • t3e
  • tcre
  • AI504783
  • CD3
  • CD3epsilon
  • T3e
  • T3E
  • TCRE
  • CD3 epsilon
  • CD3e molecule, epsilon (CD3-TCR complex)
  • CD3 antigen, epsilon polypeptide
  • CD3 molecule, epsilon
  • cd3e
  • CD3E
  • Cd3e
Reactivity
Mouse (Murine)
512
482
122
88
53
34
29
29
28
27
21
16
15
12
9
8
6
5
5
5
2
2
2
1
1
1
Host
Hamster
463
232
199
63
22
15
4
1
1
Clonality
Monoclonal
Conjugate
This CD3E antibody is conjugated to PE-Cy5
96
79
76
37
25
22
21
20
20
18
17
17
17
14
14
14
14
12
12
12
12
9
8
8
7
7
6
5
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), ELISA (Capture), ELISpot (Capture) (ELISPOT (Capture)), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Neutralization (Neut)
729
344
243
202
152
147
112
100
99
42
30
24
13
9
9
7
5
4
3
3
3
1
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN2658968
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2658969 CyTOF ELISA (Capture) ELISPOT (Capture) ICC IHC IP Neut PE-Cy5 Hamster IgG Log in to see 145-2C11 9

General

Antigen CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
Reactivity Mouse (Murine)
(512), (482), (122), (88), (53), (34), (29), (29), (28), (27), (21), (16), (15), (12), (9), (8), (6), (5), (5), (5), (2), (2), (2), (1), (1), (1)
Host Hamster
(463), (232), (199), (63), (22), (15), (4), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD3E antibody is conjugated to PE-Cy5
(96), (79), (76), (37), (25), (22), (21), (20), (20), (18), (17), (17), (17), (14), (14), (14), (14), (12), (12), (12), (12), (9), (8), (8), (7), (7), (6), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), ELISA (Capture), ELISpot (Capture) (ELISPOT (Capture)), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Neutralization (Neut)
(729), (344), (243), (202), (152), (147), (112), (100), (99), (42), (30), (24), (13), (9), (9), (7), (5), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 9 references available
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Product Details anti-CD3E Antibody

Target Details CD3E Application Details Handling ProductDetails: References for anti-CD3E antibody (ABIN2658968) Images
Purification The antibody was purified by affinity chromatography, and conjugated with PE/Cy5 under optimal conditions. The solution is free of unconjugated PE/Cy5 and unconjugated antibody.
Clone 145-2C11
Isotype IgG

Target Details CD3E

Product Details anti-CD3E Antibody Application Details Handling ProductDetails: References for anti-CD3E antibody (ABIN2658968) Images back to top
Antigen
Alternative Name CD3e (CD3E Antibody Abstract)
Background CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD3E Antibody Target Details CD3E Handling ProductDetails: References for anti-CD3E antibody (ABIN2658968) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD3E Antibody Target Details CD3E Application Details ProductDetails: References for anti-CD3E antibody (ABIN2658968) Images back to top
Concentration 0.2 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

ProductDetails: References for anti-CD3E antibody (ABIN2658968)

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling Images back to top
Background publications

Kinnaert, Pradier, Bournonville, Habrant, Goldman, Van Geertruyden et al.: "Role of CD18-dependent and CD18-independent mechanisms in the increased leukocyte adhesiveness and in the variations of circulating white blood cell populations induced by anti-CD3 monoclonal ..." in: Transplant international : official journal of the European Society for Organ Transplantation, Vol. 9, Issue 4, pp. 386-91, 1997

Henrickson, Reid, Bellet, Sawchuk, Hirsch: "Comparison of in vivo efficacy and mechanism of action of antimurine monoclonal antibodies directed against TCR alpha beta (H57-597) and CD3 (145-2C11)." in: Transplantation, Vol. 60, Issue 8, pp. 828-35, 1995

Vossen, Tibbe, Kroos, van de Winkel, Benner, Savelkoul: "Fc receptor binding of anti-CD3 monoclonal antibodies is not essential for immunosuppression, but triggers cytokine-related side effects." in: European journal of immunology, Vol. 25, Issue 6, pp. 1492-6, 1995

Weiss, Littman: "Signal transduction by lymphocyte antigen receptors." in: Cell, Vol. 76, Issue 2, pp. 263-74, 1994

Jacobs, Vandeputte, Tolkamp, de Vries, Borst, Berns: "CD3 components at the surface of pro-T cells can mediate pre-T cell development in vivo." in: European journal of immunology, Vol. 24, Issue 4, pp. 934-9, 1994

Salvadori, Gansbacher, Pizzimenti, Zier: "Abnormal signal transduction by T cells of mice with parental tumors is not seen in mice bearing IL-2-secreting tumors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 11, pp. 5176-82, 1994

Payer, Elbe, Stingl: "Circulating CD3+/T cell receptor V gamma 3+ fetal murine thymocytes home to the skin and give rise to proliferating dendritic epidermal T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 8, pp. 2536-43, 1991

Davis: "T cell receptor gene diversity and selection." in: Annual review of biochemistry, Vol. 59, pp. 475-96, 1990

Leo, Foo, Sachs, Samelson, Bluestone: "Identification of a monoclonal antibody specific for a murine T3 polypeptide." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 5, pp. 1374-8, 1987

Images

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling ProductDetails: References for anti-CD3E antibody (ABIN2658968) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) antibody (PE-Cy5) (ABIN2658968) anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) antibody (PE-Cy5)