|Application / Reactivity||Mouse (Murine)|
|Blocking Reagent (BR)||6 Antibodies|
|Cytotoxicity Test (CyTox)||3 Antibodies|
|ELISA (Capture)||6 Antibodies|
|ELISpot (Capture) (ELISPOT (Capture))||2 Antibodies|
|Flow Cytometry (FACS)||329 Antibodies|
|Fluorescence Microscopy (FM)||1 Antibodies|
|Functional Studies (Func)||17 Antibodies|
|Immunocytochemistry (ICC)||16 Antibodies|
|Immunofluorescence (IF)||38 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||22 Antibodies|
|Immunohistochemistry (IHC)||77 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||4 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||26 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||39 Antibodies|
|Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))||1 Antibodies|
|Immunoprecipitation (IP)||119 Antibodies|
|Intracellular Flow Cytometry (ICFC)||4 Antibodies|
|Antigen||CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Host||Armenian Hamster Alternatives|
|Conjugate||This CD3E antibody is un-conjugated Alternatives|
Blocking Reagent (BR), Cytotoxicity Test (CyTox), Fluorescence Microscopy (FM), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
|10 references available|
|Supplier||Log in to see|
Product Details anti-CD3E AntibodyTarget Details CD3E Application Details Handling References for anti-CD3E Antibody (ABIN1176869) Images
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Sterility||0.2 μm filtered|
|Endotoxin Level||Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.|
|Immunogen||H-2Kb specific cytotoxic T lymphocyte clone BM10-37|
Target Details CD3EProduct Details anti-CD3E Antibody Application Details Handling References for anti-CD3E Antibody (ABIN1176869) Images back to top
|Alternative Name||CD3e (CD3E Antibody Abstract)|
The 145-2C11 antibody reacts with the 25-kDa epsilon chain of the T-cell receptor-associated CD3 complex, which is expressed on thymocytes, mature T lymphocytes, and NK-T cells. The cytoplasmic domain of CD3e participates in the signal transduction events which activate several cellular biochemical pathways as a result of antigen recognition. Soluble 145-2C11 antibody can activate either unprimed (naive) or primed (memory/preactivated) T cells in vivo or in vitro, in the presence of Fc receptor-bearing accessory cells. In contrast, plate-bound 145-2C11 can activate T cells in the absence of accessory cells. Soluble 145-2C11 antibody has been reported to induce re-directed lysis of Fc receptor-bearing target cells by CTL clones and can also block lysis of specific target cells by antigen-specific CTL's. Under some conditions, T-cell activation by 145-2C11 antibody has been reported to result in apoptotic cell death. The 145-2C11 antibody does not cross-react with rat leukocytes and it has been reported that pre-incubation of thymus cell suspensions at 37°C for 2-4 hours prior to staining enhances the ability of anti-CD3epsilon and anti-alphabeta TCR mAbs to detect the T-cell receptor on immature thymocytes.
Synonyms: CD3epsilon chain
|Research Area||Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-CD3E Antibody Target Details CD3E Handling References for anti-CD3E Antibody (ABIN1176869) Images back to top
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD3E Antibody Target Details CD3E Application Details References for anti-CD3E Antibody (ABIN1176869) Images back to top
|Buffer||No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.|
|Storage Comment||Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.|
References for anti-CD3E Antibody (ABIN1176869)Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling Images back to top
|Product cited in:||
Chai, Lechler: "Immobilized anti-CD3 mAb induces anergy in murine naive and memory CD4+ T cells in vitro." in: International immunology, Vol. 9, Issue 7, pp. 935-44, 1997 (PubMed).
Wagner, Hagman, Linsley et al.: "Rescue of thymocytes from glucocorticoid-induced cell death mediated by CD28/CTLA-4 costimulatory interactions with B7-1/B7-2." in: The Journal of experimental medicine, Vol. 184, Issue 5, pp. 1631-8, 1996 (PubMed).
Nakano, Yamazaki, Miyatake et al.: "Specific interaction of topoisomerase II beta and the CD3 epsilon chain of the T cell receptor complex." in: The Journal of biological chemistry, Vol. 271, Issue 11, pp. 6483-9, 1996 (PubMed).
Salvadori, Gansbacher, Pizzimenti et al.: "Abnormal signal transduction by T cells of mice with parental tumors is not seen in mice bearing IL-2-secreting tumors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 11, pp. 5176-82, 1994 (PubMed).
Shinkai, Alt: "CD3 epsilon-mediated signals rescue the development of CD4+CD8+ thymocytes in RAG-2-/- mice in the absence of TCR beta chain expression." in: International immunology, Vol. 6, Issue 7, pp. 995-1001, 1994 (PubMed).
Vossen, Tibbe, Kroos et al.: "Fc receptor binding of anti-CD3 monoclonal antibodies is not essential for immunosuppression, but triggers cytokine-related side effects." in: European journal of immunology, Vol. 25, Issue 6, pp. 1492-6, 1995 (PubMed).
Leo, Foo, Sachs et al.: "Identification of a monoclonal antibody specific for a murine T3 polypeptide." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 5, pp. 1374-8, 1987 (PubMed).
Portoles, Rojo, Golby et al.: "Monoclonal antibodies to murine CD3 epsilon define distinct epitopes, one of which may interact with CD4 during T cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 12, pp. 4169-75, 1989 (PubMed).
Kubo, Born, Kappler et al.: "Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 8, pp. 2736-42, 1989 (PubMed).
Payer, Elbe, Stingl: "Circulating CD3+/T cell receptor V gamma 3+ fetal murine thymocytes home to the skin and give rise to proliferating dendritic epidermal T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 8, pp. 2536-43, 1991 (PubMed).