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|Application / Reactivity||Mouse (Murine)|
|Blocking Reagent (BR)||6 Antibodies|
|Cytotoxicity Test (CyTox)||3 Antibodies|
|ELISA (Capture)||6 Antibodies|
|ELISpot (Capture) (ELISPOT (Capture))||2 Antibodies|
|Flow Cytometry (FACS)||329 Antibodies|
|Fluorescence Microscopy (FM)||1 Antibodies|
|Functional Studies (Func)||17 Antibodies|
|Immunocytochemistry (ICC)||16 Antibodies|
|Immunofluorescence (IF)||38 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||22 Antibodies|
|Immunohistochemistry (IHC)||77 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||4 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||26 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||39 Antibodies|
|Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))||1 Antibodies|
|Immunoprecipitation (IP)||119 Antibodies|
|Intracellular Flow Cytometry (ICFC)||4 Antibodies|
|Antigen||CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This CD3E antibody is conjugated to FITC Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
|3 references available|
|Supplier||Log in to see|
Product Details anti-CD3E AntibodyTarget Details CD3E Application Details Handling References for anti-CD3E Antibody (ABIN135094) Images
The antibody detects: Mouse CD3 (Mr 25 kDa)
The monoclonal antibody C363.29B recognizes an epitope on the 25 kDa chain of the CD3/TCR complex.1 In the presence of Fc receptor-bearing accessory cells, soluble C363.29B can activate primed and nave T cell in vitro. Immobilized C363.29 monoclonal antibody can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present. The monoclonal antibody is cytolytic, easily used for cell surface staining and a good immunoprecipitating antibody.
Target Details CD3EProduct Details anti-CD3E Antibody Application Details Handling References for anti-CD3E Antibody (ABIN135094) Images back to top
|Alternative Name||CD3e (CD3E Antibody Abstract)|
|Background||CD3, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chain ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation.|
|Research Area||CD Antigens, Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-CD3E Antibody Target Details CD3E Handling References for anti-CD3E Antibody (ABIN135094) Images back to top
FACS: 3 µg/10^6 cells
Representative data are included in this product insert.
Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
In vitro depletion of CD3+ cells, In vitro activation of T cells
|Sample Volume||1 mL|
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD3E Antibody Target Details CD3E Application Details References for anti-CD3E Antibody (ABIN135094) Images back to top
|Buffer||Product is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS / NaN3.|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.
References for anti-CD3E Antibody (ABIN135094)Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling Images back to top
Portoles, Rojo, Golby et al.: "Monoclonal antibodies to murine CD3 epsilon define distinct epitopes, one of which may interact with CD4 during T cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 12, pp. 4169-75, 1989 (PubMed).
Dianzani, Luqman, Rojo et al.: "Molecular associations on the T cell surface correlate with immunological memory." in: European journal of immunology, Vol. 20, Issue 10, pp. 2249-57, 1990 (PubMed).
Payer, Elbe, Stingl: "Circulating CD3+/T cell receptor V gamma 3+ fetal murine thymocytes home to the skin and give rise to proliferating dendritic epidermal T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 8, pp. 2536-43, 1991 (PubMed).
ImagesProduct Details anti-CD3E Antibody Target Details CD3E Application Details Handling References for anti-CD3E Antibody (ABIN135094) back to top