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|Application / Reactivity||Human|
|Biochemical Assay (BCA)||3 Antibodies|
|Blocking Antibody (Inhibition)||1 Antibodies|
|Blocking Reagent (BR)||4 Antibodies|
|Co-Stimulation (CoSt)||2 Antibodies|
|Cytotoxicity Test (CyTox)||7 Antibodies|
|ELISA (Detection)||1 Antibodies|
|Enzyme Immunoassay (EIA)||4 Antibodies|
|Flow Cytometry (FACS)||559 Antibodies|
|Fluorescence Microscopy (FM)||1 Antibodies|
|Functional Studies (Func)||5 Antibodies|
|Immunochromatography (IC)||10 Antibodies|
|Immunocytochemistry (ICC)||42 Antibodies|
|Immunofluorescence (IF)||59 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||146 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||10 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||2 Antibodies|
|Antigen||CD8a Molecule (CD8A) Antibodies|
|Conjugate||This CD8 antibody is conjugated to PerCP-Cy5.5 Alternatives|
Flow Cytometry (FACS), Mass Cytometry (CyTOF)
|6 references available|
|Supplier||Log in to see|
Product Details anti-CD8 AntibodyTarget Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN2660510) Images
|Cross-Reactivity||Chimpanzee, Baboon, Cynomolgus, Rhesus Monkey, Pigtailed Macaque, Sooty mangabey (Cercocebus atys)|
|Purification||The antibody was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCPE/Cy5.5 and unconjugated antibody.|
Target Details CD8Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN2660510) Images back to top
|Alternative Name||CD8a (CD8A Antibody Abstract)|
|Background||CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation, and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.|
|Research Area||CD Antigens, Surface Receptors of Immune Cells, Major Histocompatibility Complex (MHC)|
Application DetailsProduct Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN2660510) Images back to top
|Application Notes||Optimal working dilution should be determined by the investigator.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN2660510) Images back to top
|Buffer||Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Protect from prolonged exposure to light. Do not freeze.|
|Storage Comment||The antibody solution should be stored undiluted between 2°C and 8°C.|
References for anti-CD8 Antibody (ABIN2660510)Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Rout, Else, Yue et al.: "Paucity of CD4+ natural killer T (NKT) lymphocytes in sooty mangabeys is associated with lack of NKT cell depletion after SIV infection." in: PLoS ONE, Vol. 5, Issue 3, pp. e9787, 2010 (PubMed).
Kmieciak, Gowda, Graham et al.: "Human T cells express CD25 and Foxp3 upon activation and exhibit effector/memory phenotypes without any regulatory/suppressor function." in: Journal of translational medicine, Vol. 7, pp. 89, 2009 (PubMed).
Thakral, Dobbins, Devine et al.: "Differential expression of the human CD8beta splice variants and regulation of the M-2 isoform by ubiquitination." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 11, pp. 7431-42, 2008 (PubMed).
Magidovich, Orr, Fass et al.: "Intrinsic disorder in the C-terminal domain of the Shaker voltage-activated K+ channel modulates its interaction with scaffold proteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 32, pp. 13022-7, 2007 (PubMed).
Mack, Tucker, Sokol et al.: "Biliary atresia is associated with CD4+ Th1 cell-mediated portal tract inflammation." in: Pediatric research, Vol. 56, Issue 1, pp. 79-87, 2004 (PubMed).
Yoshino, Ami, Terao et al.: "Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies." in: Experimental animals / Japanese Association for Laboratory Animal Science, Vol. 49, Issue 2, pp. 97-110, 2000 (PubMed).
ImagesProduct Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN2660510) back to top