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anti-Human CD8 antibody for Mass Cytometry

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • BB154331
  • CD8
  • cd8
  • Leu2
  • LOC100136450
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • MAL
  • p32
  • RHACD8-4
  • zgc:136643
Reactivity
Human
1068
538
168
73
47
42
25
23
20
20
19
16
15
14
13
12
12
11
11
10
10
8
5
3
3
2
2
2
2
2
1
1
1
Host
Mouse
1302
519
206
Clonality
Monoclonal
Conjugate
This CD8 antibody is conjugated to PerCP-Cy5.5
264
219
144
86
52
42
40
39
30
30
29
29
27
26
25
25
25
25
25
21
21
19
17
17
11
10
10
10
8
6
6
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Mass Cytometry (CyTOF)
1638
470
374
351
290
284
264
245
215
37
33
25
23
17
14
10
7
7
6
6
6
5
5
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN2660510
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008709465 ABIN2662316 FACS CyTOF Pacific Blue Mouse IgG1 kappa Log in to see RPA-T8 6
0.0008709465 ABIN2662317 FACS CyTOF Pacific Blue Mouse IgG1 kappa Log in to see RPA-T8 6
0.0008709465 ABIN2657863 FACS CyTOF Alexa Fluor 647 Mouse IgG1 kappa Log in to see RPA-T8 6
0.0008709465 ABIN2657862 FACS CyTOF Alexa Fluor 647 Mouse IgG1 kappa Log in to see RPA-T8 6
0.0008709465 ABIN2660511 FACS CyTOF PerCP-Cy5.5 Mouse IgG1 kappa Log in to see RPA-T8 6

Top referenced anti-CD8 antibody for Mass Cytometry

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Application / Reactivity Human
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ELISA 181 Antibodies
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Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 825 Antibodies
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Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 2 Antibodies

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Human
(1068), (538), (168), (73), (47), (42), (25), (23), (20), (20), (19), (16), (15), (14), (13), (12), (12), (11), (11), (10), (10), (8), (5), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1)
Host Mouse
(1302), (519), (206)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is conjugated to PerCP-Cy5.5
(264), (219), (144), (86), (52), (42), (40), (39), (30), (30), (29), (29), (27), (26), (25), (25), (25), (25), (25), (21), (21), (19), (17), (17), (11), (10), (10), (10), (8), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Mass Cytometry (CyTOF)
(1638), (470), (374), (351), (290), (284), (264), (245), (215), (37), (33), (25), (23), (17), (14), (10), (7), (7), (6), (6), (6), (5), (5), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN2660510) Images
Cross-Reactivity Chimpanzee, Baboon, Cynomolgus, Rhesus Monkey, Pigtailed Macaque, Sooty mangabey (Cercocebus atys)
Purification The antibody was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCPE/Cy5.5 and unconjugated antibody.
Clone RPA-T8
Isotype IgG1 kappa

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN2660510) Images back to top
Antigen
Alternative Name CD8a (CD8A Antibody Abstract)
Background CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation, and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.
Research Area CD Antigens, Surface Receptors of Immune Cells, Major Histocompatibility Complex (MHC)
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN2660510) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN2660510) Images back to top
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-CD8 Antibody (ABIN2660510)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Background publications

Rout, Else, Yue, Connole, Exley, Kaur: "Paucity of CD4+ natural killer T (NKT) lymphocytes in sooty mangabeys is associated with lack of NKT cell depletion after SIV infection." in: PLoS ONE, Vol. 5, Issue 3, pp. e9787, 2010

Kmieciak, Gowda, Graham, Godder, Bear, Marincola, Manjili: "Human T cells express CD25 and Foxp3 upon activation and exhibit effector/memory phenotypes without any regulatory/suppressor function." in: Journal of translational medicine, Vol. 7, pp. 89, 2009

Thakral, Dobbins, Devine, Kavathas: "Differential expression of the human CD8beta splice variants and regulation of the M-2 isoform by ubiquitination." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 11, pp. 7431-42, 2008

Magidovich, Orr, Fass, Abdu, Yifrach: "Intrinsic disorder in the C-terminal domain of the Shaker voltage-activated K+ channel modulates its interaction with scaffold proteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 32, pp. 13022-7, 2007

Mack, Tucker, Sokol, Karrer, Kotzin, Whitington, Miller: "Biliary atresia is associated with CD4+ Th1 cell-mediated portal tract inflammation." in: Pediatric research, Vol. 56, Issue 1, pp. 79-87, 2004

Yoshino, Ami, Terao, Tashiro, Honda: "Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies." in: Experimental animals / Japanese Association for Laboratory Animal Science, Vol. 49, Issue 2, pp. 97-110, 2000

Images

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Supplier Images
Flow Cytometry (FACS) image for anti-CD8 antibody (CD8a Molecule)  (PerCP-Cy5.5) (ABIN2660510) anti-CD8a Molecule (CD8A) antibody (PerCP-Cy5.5)