anti-Rat (Rattus) CD8 antibody for Functional Studies

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • cd8
  • LOC100136450
  • BB154331
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • zgc:136643
  • CD8
  • Leu2
  • MAL
  • p32
  • RHACD8-4
  • CD8 antigen, alpha chain
  • CD8 antigen, alpha polypeptide
  • CD8 alpha
  • T-cell surface glycoprotein CD8 alpha chain-like
  • CD8a molecule
  • Cd8a
  • cd8
  • LOC100136450
  • LOC100387674
  • cd8a
  • CD8A
Reactivity
Rat (Rattus)
618
525
126
92
44
26
22
19
13
13
13
12
9
5
5
5
4
4
4
2
2
1
1
Host
Mouse
790
488
198
2
1
Clonality
Monoclonal
Conjugate
This CD8 antibody is un-conjugated
151
149
93
60
48
41
40
36
31
29
24
23
23
23
23
22
20
19
19
18
15
13
11
11
11
11
10
9
9
8
7
7
7
4
4
4
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
1205
446
365
256
247
235
200
185
182
39
34
20
16
13
11
10
9
7
6
6
6
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Catalog No. ABIN1027698
$ 196.35
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2472539 Func IHC (fro) FACS IP IHC (p) WB Mouse IgG1 Log in to see OX-8 4
1 ABIN3200058 FACS Func IHC IP WB Mouse IgG1 Log in to see OX-8
1 ABIN3037655 Func FACS IP IHC WB Mouse IgG1 Log in to see

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Rat (Rattus)
(618), (525), (126), (92), (44), (26), (22), (19), (13), (13), (13), (12), (9), (5), (5), (5), (4), (4), (4), (2), (2), (1), (1)
Host Mouse
(790), (488), (198), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is un-conjugated
(151), (149), (93), (60), (48), (41), (40), (36), (31), (29), (24), (23), (23), (23), (23), (22), (20), (19), (19), (18), (15), (13), (11), (11), (11), (11), (10), (9), (9), (8), (7), (7), (7), (4), (4), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(1205), (446), (365), (256), (247), (235), (200), (185), (182), (39), (34), (20), (16), (13), (11), (10), (9), (7), (6), (6), (6), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 8 references available
Supplier Log in to see

Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 antibody (ABIN1027698) Images
Specificity The mouse monoclonal antibody OX-8 recognizes the hinge-like membrane-proximal domain of rat CD8a (32-34 kDa, alpha chain of the CD8 antigen).
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Immunogen High Mw glycoproteins from rat thymocytes
Clone OX-8
Isotype IgG1

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 antibody (ABIN1027698) Images back to top
Antigen
Alternative Name CD8A (CD8A Antibody Abstract)
Background The CD8a (CD8 alpha) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
Research Area Surface Receptors of Immune Cells, CD Antigens, Major Histocompatibility Complex (MHC)
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 antibody (ABIN1027698) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 antibody (ABIN1027698) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

References for anti-CD8 antibody (ABIN1027698)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Product cited in:

Pino, OSullivan-Murphy, Lidstone, Yang, Lipson, Jurczyk, diIorio, Brehm, Mordes, Greiner, Rossini, Bortell: "CHOP mediates endoplasmic reticulum stress-induced apoptosis in Gimap5-deficient T cells." in: PLoS ONE, Vol. 4, Issue 5, pp. e5468, 2009

Abe, Urakami, Ostanin, Zibari, Hayashida, Kitagawa, Grisham: "Induction of Foxp3-expressing regulatory T-cells by donor blood transfusion is required for tolerance to rat liver allografts." in: PLoS ONE, Vol. 4, Issue 11, pp. e7840, 2009

Katsumata, Harigai, Sugiura, Kawamoto, Kawaguchi, Matsumoto, Kohyama, Soejima, Kamatani, Hara: "Attenuation of experimental autoimmune myositis by blocking ICOS-ICOS ligand interaction." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 6, pp. 3772-9, 2007

Ishida, Usui, Yamashiro, Kaji, Amano, Ogura, Hida, Oguchi, Ambati, Miller, Gragoudas, Ng, DAmore, Shima, Adamis: "VEGF164-mediated inflammation is required for pathological, but not physiological, ischemia-induced retinal neovascularization." in: The Journal of experimental medicine, Vol. 198, Issue 3, pp. 483-9, 2003

Mitnacht, Bischof, Torres-Nagel, Hünig: "Opposite CD4/CD8 lineage decisions of CD4+8+ mouse and rat thymocytes to equivalent triggering signals: correlation with thymic expression of a truncated CD8 alpha chain in mice but not rats." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 2, pp. 700-7, 1998

Hirji, Lin, Befus: "A novel CD8 molecule expressed by alveolar and peritoneal macrophages stimulates nitric oxide production." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 4, pp. 1833-40, 1997

Torres-Nagel, Kraus, Brown, Tiefenthaler, Mitnacht, Williams, Hünig: "Differential thymus dependence of rat CD8 isoform expression." in: European journal of immunology, Vol. 22, Issue 11, pp. 2841-8, 1992

Barclay: "The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues." in: Immunology, Vol. 42, Issue 4, pp. 593-600, 1981