We are preparing the requested document. Please wait, this may take a while...!
|Application / Reactivity||Rat (Rattus)|
|Blocking Reagent (BR)||2 Antibodies|
|ELISA (Capture)||1 Antibodies|
|ELISpot (Capture) (ELISPOT (Capture))||1 Antibodies|
|Flow Cytometry (FACS)||89 Antibodies|
|Functional Studies (Func)||6 Antibodies|
|Immunoaffinity Chromatography (IAC)||2 Antibodies|
|Immunocytochemistry (ICC)||5 Antibodies|
|Immunofluorescence (IF)||6 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||22 Antibodies|
|Immunohistochemistry (IHC)||15 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||3 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||3 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||27 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||25 Antibodies|
|Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))||2 Antibodies|
|Immunoprecipitation (IP)||23 Antibodies|
|Intracellular Flow Cytometry (ICFC)||1 Antibodies|
|Antigen||CD8a Molecule (CD8A) Antibodies|
|Reactivity||Rat (Rattus) Alternatives|
|Conjugate||This CD8 antibody is un-conjugated Alternatives|
Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
|8 references available|
|Supplier||Log in to see|
Product Details anti-CD8 AntibodyTarget Details CD8 Application Details Handling References for anti-CD8 Antibody (ABIN1027698) Images
|Specificity||The mouse monoclonal antibody OX-8 recognizes the hinge-like membrane-proximal domain of rat CD8a (32-34 kDa, alpha chain of the CD8 antigen).|
|Purification||Purified from cell culture supernatant by protein-A affinity chromatography.|
|Purity||> 95 % (by SDS-PAGE)|
|Immunogen||High Mw glycoproteins from rat thymocytes|
Target Details CD8Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 Antibody (ABIN1027698) Images back to top
|Alternative Name||CD8A (CD8A Antibody Abstract)|
|Background||The CD8a (CD8 alpha) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.|
Application DetailsProduct Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 Antibody (ABIN1027698) Images back to top
Functional Application: blocking
Working concentrations should be determined by the investigator.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 Antibody (ABIN1027698) Images back to top
|Buffer||Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Do not freeze.|
|Storage Comment||Store at 2-8 °C. Do not use after expiration date stamped on vial label.|
References for anti-CD8 Antibody (ABIN1027698)Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
|Product cited in:||
Abe, Urakami, Ostanin et al.: "Induction of Foxp3-expressing regulatory T-cells by donor blood transfusion is required for tolerance to rat liver allografts." in: PLoS ONE, Vol. 4, Issue 11, pp. e7840, 2009 (PubMed).
Pino, OSullivan-Murphy, Lidstone et al.: "CHOP mediates endoplasmic reticulum stress-induced apoptosis in Gimap5-deficient T cells." in: PLoS ONE, Vol. 4, Issue 5, pp. e5468, 2009 (PubMed).
Katsumata, Harigai, Sugiura et al.: "Attenuation of experimental autoimmune myositis by blocking ICOS-ICOS ligand interaction." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 6, pp. 3772-9, 2007 (PubMed).
Ishida, Usui, Yamashiro et al.: "VEGF164-mediated inflammation is required for pathological, but not physiological, ischemia-induced retinal neovascularization." in: The Journal of experimental medicine, Vol. 198, Issue 3, pp. 483-9, 2003 (PubMed).
Mitnacht, Bischof, Torres-Nagel et al.: "Opposite CD4/CD8 lineage decisions of CD4+8+ mouse and rat thymocytes to equivalent triggering signals: correlation with thymic expression of a truncated CD8 alpha chain in mice but not rats." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 2, pp. 700-7, 1998 (PubMed).
Hirji, Lin, Befus: "A novel CD8 molecule expressed by alveolar and peritoneal macrophages stimulates nitric oxide production." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 4, pp. 1833-40, 1997 (PubMed).
Barclay: "The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues." in: Immunology, Vol. 42, Issue 4, pp. 593-600, 1981 (PubMed).
Torres-Nagel, Kraus, Brown et al.: "Differential thymus dependence of rat CD8 isoform expression." in: European journal of immunology, Vol. 22, Issue 11, pp. 2841-8, 1992 (PubMed).