anti-Rat (Rattus) CD8 antibody for Intracellular Flow Cytometry

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • cd8
  • LOC100136450
  • BB154331
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • zgc:136643
  • CD8
  • Leu2
  • MAL
  • p32
  • RHACD8-4
  • CD8 antigen, alpha chain
  • CD8 antigen, alpha polypeptide
  • CD8 alpha
  • T-cell surface glycoprotein CD8 alpha chain-like
  • CD8a molecule
  • Cd8a
  • cd8
  • LOC100136450
  • LOC100387674
  • cd8a
  • CD8A
Reactivity
Rat (Rattus)
618
525
126
92
44
26
22
19
13
13
13
12
9
5
5
5
4
4
4
2
2
1
1
Host
Mouse
790
488
198
2
1
Clonality
Monoclonal
Conjugate
This CD8 antibody is conjugated to Alexa Fluor 647
151
149
93
60
48
41
39
36
31
29
24
23
23
23
23
22
20
19
19
18
15
13
11
11
11
11
10
9
9
8
7
7
7
4
4
4
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Intracellular Flow Cytometry (ICFC)
1206
446
365
257
248
236
200
185
182
39
35
19
16
13
11
11
9
7
6
6
6
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN2657861
Contact our Customer Service for availability and price in your country.

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Rat (Rattus)
(618), (525), (126), (92), (44), (26), (22), (19), (13), (13), (13), (12), (9), (5), (5), (5), (4), (4), (4), (2), (2), (1), (1)
Host Mouse
(790), (488), (198), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is conjugated to Alexa Fluor 647
(151), (149), (93), (60), (48), (41), (39), (36), (31), (29), (24), (23), (23), (23), (23), (22), (20), (19), (19), (18), (15), (13), (11), (11), (11), (11), (10), (9), (9), (8), (7), (7), (7), (4), (4), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Intracellular Flow Cytometry (ICFC)
(1206), (446), (365), (257), (248), (236), (200), (185), (182), (39), (35), (19), (16), (13), (11), (11), (9), (7), (6), (6), (6), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 8 references available
Supplier Log in to see

Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 antibody (ABIN2657861) Images
Purification The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Clone OX-8
Isotype IgG1 kappa

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 antibody (ABIN2657861) Images back to top
Antigen
Alternative Name CD8a (CD8A Antibody Abstract)
Background CD8a is a 32 kD glycoprotein also known as T8, Lyt2, Ly-2, and CD8α. CD8a is a member of the immunoglobulin superfamily expressed on most thymocytes, subset of mature T cells, most NK cells, macrophages, and some activated CD4+ T cells (not resting). CD8a forms heterodimers with the CD8β chain (CD8b) on the surface of most thymocytes, while mature peripheral T lymphocytes express almost exclusively the CD8 αβ heterodimer. Intestinal intraepithelial lymphocytes express CD8a without CD8b. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 participates in T cell activation through its association with the T cell receptor complex and protein tyrosine kinase lck (p56lck).
Research Area CD Antigens, Surface Receptors of Immune Cells, Major Histocompatibility Complex (MHC)
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 antibody (ABIN2657861) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 antibody (ABIN2657861) Images back to top
Concentration 0.5 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C

References for anti-CD8 antibody (ABIN2657861)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Background publications

Mitnacht, Bischof, Torres-Nagel, Hünig: "Opposite CD4/CD8 lineage decisions of CD4+8+ mouse and rat thymocytes to equivalent triggering signals: correlation with thymic expression of a truncated CD8 alpha chain in mice but not rats." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 2, pp. 700-7, 1998

Hirji, Lin, Befus: "A novel CD8 molecule expressed by alveolar and peritoneal macrophages stimulates nitric oxide production." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 4, pp. 1833-40, 1997

Wallgren, Karlsson-Parra, Korsgren: "The main infiltrating cell in xenograft rejection is a CD4+ macrophage and not a T lymphocyte." in: Transplantation, Vol. 60, Issue 6, pp. 594-601, 1995

Torres-Nagel, Kraus, Brown, Tiefenthaler, Mitnacht, Williams, Hünig: "Differential thymus dependence of rat CD8 isoform expression." in: European journal of immunology, Vol. 22, Issue 11, pp. 2841-8, 1992

Johnson, Gagnon, Barclay, Williams: "Purification, chain separation and sequence of the MRC OX-8 antigen, a marker of rat cytotoxic T lymphocytes." in: The EMBO journal, Vol. 4, Issue 10, pp. 2539-45, 1985

Thomas, Green: "Molecular nature of the W3/25 and MRC OX-8 marker antigens for rat T lymphocytes: comparisons with mouse and human antigens." in: European journal of immunology, Vol. 13, Issue 10, pp. 855-8, 1984

Mason, Arthur, Dallman, Green, Spickett, Thomas: "Functions of rat T-lymphocyte subsets isolated by means of monoclonal antibodies." in: Immunological reviews, Vol. 74, pp. 57-82, 1983

Barclay: "The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues." in: Immunology, Vol. 42, Issue 4, pp. 593-600, 1981

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 antibody (ABIN2657861) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD8a Molecule (CD8A) antibody (Alexa Fluor 647) (ABIN2657861) anti-CD8a Molecule (CD8A) antibody (Alexa Fluor 647)