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anti-Human CD83 antibody for Immunofluorescence

Recommended CD83 Antibody (supplied by: Log in to see )

Antigen
CD83 Antibodies
  • BL11
  • HB15
Reactivity
Human
139
80
34
7
5
5
5
5
Host
Mouse
91
63
47
3
Clonality
Monoclonal
Conjugate
This CD83 antibody is un-conjugated
29
25
12
7
6
6
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
121
49
31
27
22
20
11
9
5
5
2
2
2
2
1
Supplier
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Catalog No. ABIN263553
$ 632.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010850558 ABIN2659323 FACS IF IP IHC PE-Cy7 Mouse IgG1 kappa Log in to see HB15e 7
0.0010850558 ABIN2659753 FACS IF PE/Dazzle™ 594 Mouse IgG1 kappa Log in to see HB15e 5
0.0010850558 ABIN211881 FACS IF IHC Mouse IgG2b, kappa Log in to see
0.0010850558 ABIN2659324 FACS IF IP IHC PE-Cy7 Mouse IgG1 kappa Log in to see HB15e 7
0.0010850558 ABIN2659754 FACS IF PE/Dazzle™ 594 Mouse IgG1 kappa Log in to see HB15e 5
0.0010850558 ABIN2472640 IHC (fro) FACS IF IHC (p) Mouse IgG1 Log in to see HB15e 4
0.0010850558 ABIN2851653 FACS IF FITC Mouse IgG1 Log in to see
0.0010850558 ABIN2851723 FACS IF PE Mouse IgG1 Log in to see
0.0010850558 ABIN2708055 FACS IF Mouse IgG1 Log in to see
0.0010850558 ABIN2229035 FACS IF IHC Alkaline Phosphatase (AP) Mouse IgG2b kappa Log in to see 1-BB-583
0.0010850558 ABIN2229036 FACS IF IHC APC Mouse IgG2b kappa Log in to see 1-BB-583
0.0010850558 ABIN2229038 FACS IF IHC FITC Mouse IgG2b kappa Log in to see 1-BB-583
0.0010850558 ABIN2229040 FACS IF IHC PE Mouse IgG2b kappa Log in to see 1-BB-583
0.0010850558 ABIN2229037 FACS IF IHC Biotin Mouse IgG2b kappa Log in to see 1-BB-583
0.0010850558 ABIN2229021 FACS IF IHC Mouse IgG2b kappa Log in to see 1-BB-583
0.0010850558 ABIN3037621 ICC FACS IF FITC Mouse IgG1 Log in to see
0.0010850558 ABIN3037622 ICC FACS IF PE Mouse IgG1 Log in to see
0.0010850558 ABIN3067118 ICC FACS IF Mouse IgG1 Log in to see

Top referenced anti-CD83 antibody for Immunofluorescence

Similar anti-CD83 Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 2 Antibodies
ELISA 21 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 82 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 19 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 30 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 6 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 9 Antibodies
Immunoprecipitation (IP) 7 Antibodies
Western Blotting (WB) 44 Antibodies

General

Antigen CD83 Antibodies
Reactivity Human
(139), (80), (34), (7), (5), (5), (5), (5)
Host Mouse
(91), (63), (47), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD83 antibody is un-conjugated
(29), (25), (12), (7), (6), (6), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
(121), (49), (31), (27), (22), (20), (11), (9), (5), (5), (2), (2), (2), (2), (1)
Pubmed 6 references available
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Product Details anti-CD83 Antibody

Target Details CD83 Application Details Handling References for anti-CD83 Antibody (ABIN263553) Images
Specificity This antibody reacts to CD83.
Characteristics Synonyms: HB15, Cell surface protein HB15, B-cell activation protein
Purification Ion exchange or affinity chromatography
Immunogen COS cells transfected with the HB15 cDNA. Hybridization of NS1 myeloma cells with spleencells from Balb/c mice.
Clone HB15A
Isotype IgG2b

Target Details CD83

Product Details anti-CD83 Antibody Application Details Handling References for anti-CD83 Antibody (ABIN263553) Images back to top
Antigen
Alternative Name CD83 (CD83 Antibody Abstract)
Background The CD83 antigen is a 186 amino acid single chain glycoprotein. This molecule is a member of the immunoglobulin superfamily and is composed of an extracellular V type immunoglobulin-like single domain, a transmembrane region, and a short, 40 amino acid cytoplasmic tail. CD83 antigen undergoes extensive post translational glycosylation, as the determined Mr is twice the predicted size of the core protein. However, CD83+ cells have a unique cell surface immunophenotype that does not correlate with that of T cells, B cells, NK cells, or cells of the myelomonocytic lineage. CD83+ cells coexpress the highest levels of MHC class II molecules, when compared with other leucocyte lineages. They also coexpress T cell markers (CD2, CD5), B cell markers (CD40, CD78), myeloid cell markers (CD13, CD33, CD36) and cytokine receptors, as well as other cell surface molecules.Synonyms: B-cell activation protein, Cell surface protein HB15, HB15
Gene ID 9308
UniProt Q01151
Research Area Immunology, CD Antigens, Adaptive Immunity, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD83 Antibody Target Details CD83 Handling References for anti-CD83 Antibody (ABIN263553) Images back to top
Application Notes Fluorescent microscopy or flow cytometry, after dendritic cells enrichment by culture,enumeration and/or sorting of CD83+ cells. Studies of human dendritic cell subpopulations in tissues by immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD83 Antibody Target Details CD83 Application Details References for anti-CD83 Antibody (ABIN263553) Images back to top
Reconstitution Restore with 1 mL of distilled water
Buffer PBS containing 1 mg/mL BSA
Storage 4 °C/-20 °C
Storage Comment Prior to reconstitution store at 2-8 °C. e addition of 0. 1% (w/v) sodium azide is recommended for storage of the reconstitutedform for up to one month at 2-8 °C. Following reconstitution store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

References for anti-CD83 Antibody (ABIN263553)

Product Details anti-CD83 Antibody Target Details CD83 Application Details Handling Images back to top
Product cited in:

Ampem-Lassen, Sidiroglou, Peng, Huntington, Roberts: "Index mapping for fibres with symmetric and asymmetric refractive index profiles." in: Optics express, Vol. 16, Issue 15, pp. 10912-7, 2008

Fries, Heukeshoven, Hauber, Grüttner, Stocking, Kehlenbach, Hauber, Chemnitz: "Analysis of nucleocytoplasmic trafficking of the HuR ligand APRIL and its influence on CD83 expression." in: The Journal of biological chemistry, Vol. 282, Issue 7, pp. 4504-15, 2007

Prechtel, Chemnitz, Schirmer, Ehlers, Langbein-Detsch, Stülke, Dabauvalle, Kehlenbach, Hauber: "Expression of CD83 is regulated by HuR via a novel cis-active coding region RNA element." in: The Journal of biological chemistry, Vol. 281, Issue 16, pp. 10912-25, 2006

Background publications

Zhou, Tedder: "Human blood dendritic cells selectively express CD83, a member of the immunoglobulin superfamily." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 8, pp. 3821-35, 1995

Zhou, Tedder: "A distinct pattern of cytokine gene expression by human CD83+ blood dendritic cells." in: Blood, Vol. 86, Issue 9, pp. 3295-301, 1995

Zhou, Schwarting, Smith, Tedder: "A novel cell-surface molecule expressed by human interdigitating reticulum cells, Langerhans cells, and activated lymphocytes is a new member of the Ig superfamily." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 2, pp. 735-42, 1992