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|Application / Reactivity||Human|
|Biochemical Assay (BCA)||2 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies|
|Flow Cytometry (FACS)||81 Antibodies|
|Immunocytochemistry (ICC)||4 Antibodies|
|Immunofluorescence (IF)||19 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||29 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||6 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||8 Antibodies|
|Immunoprecipitation (IP)||7 Antibodies|
|Western Blotting (WB)||44 Antibodies|
|Conjugate||This CD83 antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
|6 references available|
|Supplier||Log in to see|
Product Details anti-CD83 AntibodyTarget Details CD83 Application Details Handling References for anti-CD83 Antibody (ABIN263553) Images
|Specificity||This antibody reacts to CD83.|
|Characteristics||Synonyms: HB15, Cell surface protein HB15, B-cell activation protein|
|Purification||Ion exchange or affinity chromatography|
|Immunogen||COS cells transfected with the HB15 cDNA. Hybridization of NS1 myeloma cells with spleencells from Balb/c mice.|
Target Details CD83Product Details anti-CD83 Antibody Application Details Handling References for anti-CD83 Antibody (ABIN263553) Images back to top
|Alternative Name||CD83 (CD83 Antibody Abstract)|
|Background||The CD83 antigen is a 186 amino acid single chain glycoprotein. This molecule is a member of the immunoglobulin superfamily and is composed of an extracellular V type immunoglobulin-like single domain, a transmembrane region, and a short, 40 amino acid cytoplasmic tail. CD83 antigen undergoes extensive post translational glycosylation, as the determined Mr is twice the predicted size of the core protein. However, CD83+ cells have a unique cell surface immunophenotype that does not correlate with that of T cells, B cells, NK cells, or cells of the myelomonocytic lineage. CD83+ cells coexpress the highest levels of MHC class II molecules, when compared with other leucocyte lineages. They also coexpress T cell markers (CD2, CD5), B cell markers (CD40, CD78), myeloid cell markers (CD13, CD33, CD36) and cytokine receptors, as well as other cell surface molecules.Synonyms: B-cell activation protein, Cell surface protein HB15, HB15|
|Research Area||Immunology, CD Antigens, Adaptive Immunity, Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-CD83 Antibody Target Details CD83 Handling References for anti-CD83 Antibody (ABIN263553) Images back to top
Fluorescent microscopy or flow cytometry, after dendritic cells enrichment by culture,enumeration and/or sorting of CD83+ cells. Studies of human dendritic cell subpopulations in tissues by immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD83 Antibody Target Details CD83 Application Details References for anti-CD83 Antibody (ABIN263553) Images back to top
|Reconstitution||Restore with 1 mL of distilled water|
|Buffer||PBS containing 1 mg/mL BSA|
|Storage||4 °C/-20 °C|
Prior to reconstitution store at 2-8 °C. e addition of 0. 1% (w/v) sodium azide is recommended for storage of the reconstitutedform for up to one month at 2-8 °C. Following reconstitution store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|
References for anti-CD83 Antibody (ABIN263553)Product Details anti-CD83 Antibody Target Details CD83 Application Details Handling Images back to top
|Product cited in:||
Ampem-Lassen, Sidiroglou, Peng et al.: "Index mapping for fibres with symmetric and asymmetric refractive index profiles." in: Optics express, Vol. 16, Issue 15, pp. 10912-7, 2008 (PubMed).
Fries, Heukeshoven, Hauber et al.: "Analysis of nucleocytoplasmic trafficking of the HuR ligand APRIL and its influence on CD83 expression." in: The Journal of biological chemistry, Vol. 282, Issue 7, pp. 4504-15, 2007 (PubMed).
Prechtel, Chemnitz, Schirmer et al.: "Expression of CD83 is regulated by HuR via a novel cis-active coding region RNA element." in: The Journal of biological chemistry, Vol. 281, Issue 16, pp. 10912-25, 2006 (PubMed).
Zhou, Tedder: "Human blood dendritic cells selectively express CD83, a member of the immunoglobulin superfamily." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 8, pp. 3821-35, 1995 (PubMed).
Zhou, Tedder: "A distinct pattern of cytokine gene expression by human CD83+ blood dendritic cells." in: Blood, Vol. 86, Issue 9, pp. 3295-301, 1995 (PubMed).
Zhou, Schwarting, Smith et al.: "A novel cell-surface molecule expressed by human interdigitating reticulum cells, Langerhans cells, and activated lymphocytes is a new member of the Ig superfamily." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 2, pp. 735-42, 1992 (PubMed).