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anti-Human Cytoplasmic Protein NCK1 antibody for Enzyme Immunoassay

Recommended Cytoplasmic Protein NCK1 Antibody (supplied by: Log in to see )

Antigen
NCK Adaptor Protein 1 (NCK1) Antibodies
  • 6330586M15Rik
  • D230010O13Rik
  • Nck
  • NCK
  • Nck-1
  • nck-1
  • NCK1
  • NCKalpha
Epitope
N-Term
35
15
5
4
3
2
2
2
2
2
1
1
1
1
1
Reactivity
Human
95
19
17
3
2
2
2
2
Host
Rabbit
70
25
1
Clonality
Polyclonal
Conjugate
This Cytoplasmic Protein NCK1 antibody is un-conjugated
4
4
4
4
4
4
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
90
52
41
13
10
8
7
3
1
1
1
Supplier
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Catalog No. ABIN453756
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008833851 ABIN1108388 EIA FACS IHC (p) WB Mouse IgG1 Log in to see 5B7 2

Top referenced anti-Cytoplasmic Protein NCK1 antibody for Enzyme Immunoassay

Similar anti-Cytoplasmic Protein NCK1 Antibodies

Application / Reactivity Human
ELISA 52 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 9 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 14 Antibodies
Immunohistochemistry (IHC) 42 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 11 Antibodies
Immunoprecipitation (IP) 7 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 91 Antibodies

General

Antigen NCK Adaptor Protein 1 (NCK1) Antibodies
Epitope N-Term
(35), (15), (5), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human
(95), (19), (17), (3), (2), (2), (2), (2)
Host Rabbit
(70), (25), (1)
Clonality
Polyclonal
Conjugate This Cytoplasmic Protein NCK1 antibody is un-conjugated
(4), (4), (4), (4), (4), (4)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(90), (52), (41), (13), (10), (8), (7), (3), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-Cytoplasmic Protein NCK1 Antibody

Target Details Cytoplasmic Protein NCK1 Application Details Handling References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN453756) Images
Specificity This antibody reacts to NCK1.
Purification Affinity chromatography on Protein A
Immunogen KLH conjugated synthetic peptide selected from the N-terminal region of human NCK1

Target Details Cytoplasmic Protein NCK1

Product Details anti-Cytoplasmic Protein NCK1 Antibody Application Details Handling References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN453756) Images back to top
Antigen
Alternative Name NCK1 (NCK1 Antibody Abstract)
Background NCK1 is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS.Synonyms: Cytoplasmic protein NCK1, NCK, NCK adaptor protein 1, SH2/SH3 adaptor protein NCK-alpha
Gene ID 4690
NCBI Accession NP_006144
UniProt P16333
Pathways TCR Signaling

Application Details

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Handling References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN453756) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN453756) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN453756)

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details Handling Images back to top
Background publications

Meisenhelder, Hunter: "The SH2/SH3 domain-containing protein Nck is recognized by certain anti-phospholipase C-gamma 1 monoclonal antibodies, and its phosphorylation on tyrosine is stimulated by platelet-derived growth factor and epidermal growth factor treatment." in: Molecular and cellular biology, Vol. 12, Issue 12, pp. 5843-56, 1992

Images

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details Handling References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN453756) back to top
Supplier Images
Western Blotting (WB) image for anti-Cytoplasmic Protein NCK1 antibody (NCK Adaptor Protein 1) (N-Term) (ABIN453756) Western blot analysis of NCK1 Antibody (N-term) in Hela cell line lysates (35ug/lane)...