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anti-Human Cytoplasmic Protein NCK1 antibody for Immunohistochemistry

Recommended Cytoplasmic Protein NCK1 Antibody (supplied by: Log in to see )

Antigen
NCK Adaptor Protein 1 (NCK1) Antibodies
  • 6330586M15Rik
  • D230010O13Rik
  • Nck
  • NCK
  • Nck-1
  • nck-1
  • NCK1
  • NCKalpha
Reactivity
Human, Monkey
117
20
18
2
2
2
2
2
1
Host
Mouse
72
45
1
Clonality
Monoclonal
Conjugate
This Cytoplasmic Protein NCK1 antibody is un-conjugated
5
5
5
5
4
4
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
112
72
42
14
12
8
7
4
2
1
1
Supplier
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Catalog No. ABIN969545
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.22915025 ABIN189565 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG Log in to see Y531
0.22915025 ABIN2446711 IHC WB Rabbit Ig Fraction N-Term Log in to see Polyclonal
0.22915025 ABIN2428459 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.22915025 ABIN2421903 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.22915025 ABIN1845767 FACS IHC ELISA WB Mouse IgG1 Log in to see
0.0011319318 ABIN967743 IF IP IHC WB Mouse IgG2b AA 279-377 Log in to see 108-NCK 5
0.0011319318 ABIN967744 IF IP IHC WB Mouse IgG2b AA 279-377 Log in to see 108-NCK 5
0.0011319318 ABIN1499651 FACS IHC ELISA WB Mouse IgG1 Log in to see 5B7
0.0011319318 ABIN1873840 IHC WB Rabbit IgG Log in to see Polyclonal
0.0011319318 ABIN1873839 IF IHC WB Rabbit IgG Log in to see Polyclonal
0.0011319318 ABIN3021268 IHC WB Rabbit IgG Log in to see Polyclonal
0.0011319318 ABIN1916308 IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG N-Term, AA 62-92 Log in to see Polyclonal
0.0011319318 ABIN1916309 IHC ELISA WB APC Rabbit IgG N-Term, AA 62-92 Log in to see Polyclonal
0.0011319318 ABIN1916310 IHC ELISA WB Biotin Rabbit IgG N-Term, AA 62-92 Log in to see Polyclonal
0.0011319318 ABIN1916311 IHC ELISA WB FITC Rabbit IgG N-Term, AA 62-92 Log in to see Polyclonal
0.0011319318 ABIN1916312 IHC ELISA WB PE Rabbit IgG N-Term, AA 62-92 Log in to see Polyclonal
0.0011319318 ABIN2893981 IHC Rabbit Log in to see Polyclonal
0.0011319318 ABIN1916313 IHC ELISA WB HRP Rabbit IgG N-Term, AA 62-92 Log in to see Polyclonal
0.0011319318 ABIN2318044 IHC ELISA WB Rabbit IgG N-Term, AA 62-92 Log in to see Polyclonal
0.0011319318 ABIN2318068 IHC ELISA WB FITC Rabbit IgG N-Term, AA 62-92 Log in to see Polyclonal

Top referenced anti-Cytoplasmic Protein NCK1 antibody for Immunohistochemistry

Similar anti-Cytoplasmic Protein NCK1 Antibodies

Application / Reactivity Monkey Human
ELISA 2 Antibodies 73 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 2 Antibodies
Flow Cytometry (FACS) 3 Antibodies 10 Antibodies
Immunohistochemistry (IHC) 2 Antibodies 44 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 1 Antibodies 13 Antibodies
Western Blotting (WB) 3 Antibodies 113 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (IF) 15 Antibodies
Immunoprecipitation (IP) 7 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies

General

Antigen NCK Adaptor Protein 1 (NCK1) Antibodies
Reactivity Human, Monkey
(117), (20), (18), (2), (2), (2), (2), (2), (1)
Host Mouse
(72), (45), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Cytoplasmic Protein NCK1 antibody is un-conjugated
(5), (5), (5), (5), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(112), (72), (42), (14), (12), (8), (7), (4), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-Cytoplasmic Protein NCK1 Antibody

Target Details Cytoplasmic Protein NCK1 Application Details Handling References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN969545) Images
Purification Purified
Immunogen Purified recombinant fragment of human NCK1 expressed in E. coli.
Clone 5B7
Isotype IgG1

Target Details Cytoplasmic Protein NCK1

Product Details anti-Cytoplasmic Protein NCK1 Antibody Application Details Handling References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN969545) Images back to top
Antigen
Alternative Name NCK1 (NCK1 Antibody Abstract)
Background The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found.
Molecular Weight 47 kDa
Gene ID 4690
HGNC 4690
Pathways TCR Signaling

Application Details

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Handling References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN969545) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN969545) Images back to top
Format Liquid
Buffer PBS with 0.05 % sodium azide and 0.5 % protein stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN969545)

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details Handling Images back to top
Product cited in:

Preisinger, Kolch: "The Bcr-Abl kinase regulates the actin cytoskeleton via a GADS/Slp-76/Nck1 adaptor protein pathway." in: Cellular signalling, Vol. 22, Issue 5, pp. 848-56, 2010

Blasutig, New, Thanabalasuriar, Dayarathna, Goudreault, Quaggin, Li, Gruenheid, Jones, Pawson: "Phosphorylated YDXV motifs and Nck SH2/SH3 adaptors act cooperatively to induce actin reorganization." in: Molecular and cellular biology, Vol. 28, Issue 6, pp. 2035-46, 2008

Images

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details Handling References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN969545) back to top
Supplier Images
ELISA image for anti-Cytoplasmic Protein NCK1 antibody (NCK Adaptor Protein 1) (ABIN969545) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-Cytoplasmic Protein NCK1 antibody (NCK Adaptor Protein 1) (ABIN969545) Figure 2: Flow cytometric analysis of Jurkat cells using NCK1 mouse mAb (green) and n...
Immunohistochemistry (IHC) image for anti-Cytoplasmic Protein NCK1 antibody (NCK Adaptor Protein 1) (ABIN969545) Figure 3: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues usi...
Western Blotting (WB) image for anti-Cytoplasmic Protein NCK1 antibody (NCK Adaptor Protein 1) (ABIN969545) Figure 4: Western blot analysis using NCK1 mouse mAb against Jurkat (1), HeLa (2), HE...
Western Blotting (WB) image for anti-Cytoplasmic Protein NCK1 antibody (NCK Adaptor Protein 1) (ABIN969545) Figure 5: Western blot analysis using NCK1 mAb against human NCK1 (AA: 203-371) recom...