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anti-Human Cytoplasmic Protein NCK1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Cytoplasmic Protein NCK1 Antibody (supplied by: Log in to see )

Antigen
NCK Adaptor Protein 1 (NCK1) Antibodies
  • 6330586M15Rik
  • D230010O13Rik
  • Nck
  • NCK
  • Nck-1
  • nck-1
  • NCK1
  • NCKalpha
Epitope
AA 63-92, N-Term
35
24
15
4
3
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
117
20
18
3
2
2
2
2
1
Host
Rabbit
71
46
1
Clonality
Polyclonal
Conjugate
This Cytoplasmic Protein NCK1 antibody is un-conjugated
5
5
5
5
4
4
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
112
73
43
14
11
9
7
4
2
1
1
Supplier
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Catalog No. ABIN657648
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089735596 ABIN1108388 EIA FACS IHC (p) WB Mouse IgG1 Log in to see 5B7 2
0.00089735596 ABIN1499652 ICC IF IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.00089735596 ABIN957751 IHC (p) ELISA WB Mouse IgG1, kappa Log in to see 11D10
0.00089735596 ABIN957794 IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.00089735596 ABIN189565 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG Log in to see Y531
0.00089735596 ABIN1724470 IHC (p) ELISA WB Mouse IgG1 kappa Log in to see 11D10
0.00089735596 ABIN1811813 IHC (p) WB Rabbit AA 62-92 Log in to see Polyclonal
0.00089735596 ABIN1737225 IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.00089735596 ABIN4338264 ELISA IHC (p) WB Mouse IgG1 kappa Log in to see 11D10
0.00089735596 ABIN4338265 ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal
0.00089735596 ABIN2770529 IHC (p) IHC ELISA WB Mouse IgG1, kappa Log in to see 11D10
0.00089735596 ABIN2764735 IHC (p) WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-Cytoplasmic Protein NCK1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-Cytoplasmic Protein NCK1 Antibodies

Application / Reactivity Human
ELISA 73 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 10 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (IF) 15 Antibodies
Immunohistochemistry (IHC) 44 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 13 Antibodies
Immunoprecipitation (IP) 7 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 113 Antibodies

General

Antigen NCK Adaptor Protein 1 (NCK1) Antibodies
Epitope AA 63-92, N-Term
(35), (24), (15), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(117), (20), (18), (3), (2), (2), (2), (2), (1)
Host Rabbit
(71), (46), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This Cytoplasmic Protein NCK1 antibody is un-conjugated
(5), (5), (5), (5), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(112), (73), (43), (14), (11), (9), (7), (4), (2), (1), (1)
Pubmed 3 references available
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Product Details anti-Cytoplasmic Protein NCK1 Antibody

Target Details Cytoplasmic Protein NCK1 Application Details Handling References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN657648) Images
Specificity This NCK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-92 amino acids from the N-terminal region of human NCK1.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This NCK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-92 AA from the N-terminal region of human NCK1.
Clone RB33697
Isotype Ig

Target Details Cytoplasmic Protein NCK1

Product Details anti-Cytoplasmic Protein NCK1 Antibody Application Details Handling References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN657648) Images back to top
Antigen
Alternative Name NCK1 (NCK1 Antibody Abstract)
Background The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found.
Synonyms: Cytoplasmic protein NCK1,NCK1,NCK
Molecular Weight 42864 DA
Gene ID 4690
NCBI Accession NP_006144, NP_001177725
UniProt P16333
Research Area Translation Factors, Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure
Pathways TCR Signaling

Application Details

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Handling References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN657648) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN657648) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment NCK1 Antibody (N-term) can be refrigerated at 2-8 °C for up to 6 months. For long term storage, place the at -20 °C.
Expiry Date 6 months

References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN657648)

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details Handling Images back to top
Background publications

Barda-Saad, Shirasu, Pauker, Hassan, Perl, Balbo, Yamaguchi, Houtman, Appella, Schuck, Samelson: "Cooperative interactions at the SLP-76 complex are critical for actin polymerization." in: The EMBO journal, Vol. 29, Issue 14, pp. 2315-28, 2010

Gehmlich, Hayess, Legler, Haebel, Van der Ven, Ehler, Fürst: "Ponsin interacts with Nck adapter proteins: implications for a role in cytoskeletal remodelling during differentiation of skeletal muscle cells." in: European journal of cell biology, Vol. 89, Issue 5, pp. 351-64, 2010

Preisinger, Kolch: "The Bcr-Abl kinase regulates the actin cytoskeleton via a GADS/Slp-76/Nck1 adaptor protein pathway." in: Cellular signalling, Vol. 22, Issue 5, pp. 848-56, 2010

Images

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details Handling References for anti-Cytoplasmic Protein NCK1 Antibody (ABIN657648) back to top
Supplier Images
Western Blotting (WB) image for anti-Cytoplasmic Protein NCK1 antibody (NCK Adaptor Protein 1) (AA 63-92) (ABIN657648) Western blot analysis of NCK1 (arrow) using rabbit polyclonal NCK1 Antibody (N-term) ...