|Application / Reactivity||Rat (Rattus)||Mouse (Murine)||Human|
|ELISA||7 Antibodies||6 Antibodies||52 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies||1 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies||3 Antibodies|
|Immunofluorescence (IF)||7 Antibodies||8 Antibodies|
|Immunohistochemistry (IHC)||14 Antibodies||14 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||2 Antibodies||3 Antibodies|
|Immunoprecipitation (IP)||4 Antibodies||3 Antibodies|
|Western Blotting (WB)||17 Antibodies||19 Antibodies|
|Antigen||NCK Adaptor Protein 1 (NCK1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Cytoplasmic Protein NCK1 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
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Product Details anti-Cytoplasmic Protein NCK1 AntibodyTarget Details Cytoplasmic Protein NCK1 Application Details Handling Images
|Immunogen||A synthetic peptide corresponding to residues close to the SH2 domain of human Nck was used as immunogen.|
Target Details Cytoplasmic Protein NCK1Product Details anti-Cytoplasmic Protein NCK1 Antibody Application Details Handling Images back to top
|Alternative Name||NCK1 (NCK1 Antibody Abstract)|
Nck is a ubiquitously expressed adaptor protein consisting of one SH2 and three SH3Src homology domains. Stimulation by epidermal growth factor (EGF) or platelet-derivedgrowth factor (PDGF) leads to phosphorylation of Nck on tyrosine, serine and threonineresidues. Overexpression of Nck resulted in transformation of NIH 3T3 and 3Y1 ratfibroblast (1-2). Additionally, Nck has been shown to associate with BLNK, PLCG1 andVAV1 in a B-cell antigen receptor-dependent fashion and may play a role in linkingtyrosine and serine/threonine kinase pathways within the cell (2). Alternate Names: anti-Cytoplasmic protein NCK1 antibody, anti-Melanoma Nck protein antibody,anti-MGC12668 antibody, anti-NCK 1 antibody, anti-Nck adaptor protein 1 antibody,anti-NCK tyrosine kinase antibody, anti-NCK1 antibody, anti-NCKalpha antibody,anti-Non catalytic region of tyrosine kinase antibody, anti-SH2/SH3 adaptor protein NCKalpha antibody, anti-SH2/SH3 adaptor protein NCKalpha antibody.
Gene Symbol: NCK1
|Gene ID||4690, 17973, 300955|
Application DetailsProduct Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Handling Images back to top
This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation, Flow Cytometry. In Western blot this antibody detects a band at approximately 47kDa.
Recommended dilutions: Flow Cytometry 1:60, Immunocytochemistry/Immunofluorescence 1:250-500, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:100-250, Immunoprecipitation 1:50, Western Blot 1:500-10000
Protocol specific for Nck Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
|Restrictions||For Research Use only|
HandlingProduct Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details Images back to top
|Buffer||PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage Comment||-20 °C.|
ImagesProduct Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details Handling back to top